10x objectives for multiphoton work?

[Daniel James White]

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Hi Adrian,

Zeiss have a water immersion 10x / 0.45

ziess objective database says its 65% at 1000 nm.
C-Apochromat 10x/0.45 W M27

it expects a 0.17 coverglass....so you must look through a window....but
1.8 mm Working distance...

didnt try it for 2 photon yet.... we have it on a widefield system.

Dan


On Sep 23, 2011, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:

>
> Date:    Thu, 22 Sep 2011 17:13:54 +1000
> From:    Adrian Smith <[hidden email]>
> Subject: 10x objectives for multiphoton work?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
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>
> Hi all,
>
> Just wondering if anyone has experience with any 10x objectives for =
> intravital MP work?
>
> Not fussy about manufacturer at this point - just looking for =
> recommendations.=20
>
> (e.g. I can see 10x NA0.45 Plan Apo objectives in the Nikon and (maybe) =
> Zeiss catalogs=85 and there is even a NA0.5 Fluor in those as well).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

Dr. Daniel James White BSc. (Hons.) PhD

Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.

Max Planck Institute of Molecular Cell Biology and Genetics
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[Pascal Weber]

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There is no 10X N: 0.45 water immersion Zeiss. I used it on a 2P. It is really
good but less than the 20X water immersion. With which we have obtained
more than 980micron deep in adult mouse brain (Dextran-marking rhoamine).
Be careful not to put water on the front otherwise very poor results. There is
a 10X water immersion but really not very good.

[Zac Arrac Atelaz]

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There is a 25x water inmersion in Olympus, with 82% transmittance from 400nm to 1000nm, so you can imagine that this can easily can go down about 1mm or so, on a regular basis, this one is designed specially for deep imaging and 2P, there is some transparent embryo technique that can achieve even more thant that
 
Regards
 
Gabriel OH
 

> Date: Tue, 27 Sep 2011 05:24:23 -0500
> From: [hidden email]
> Subject: Re: 10x objectives for multiphoton work?
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> There is no 10X N: 0.45 water immersion Zeiss. I used it on a 2P. It is really
> good but less than the 20X water immersion. With which we have obtained
> more than 980micron deep in adult mouse brain (Dextran-marking rhoamine).
> Be careful not to put water on the front otherwise very poor results. There is
> a 10X water immersion but really not very good.