160x GSD objective in a conventional confocal ?

[Renato A. Mortara]

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Dear all

 

Our institution has just acquired a GSD system from Leica that is based on a
160x 1.43 objective that sits in a ‘heavy’ stage they named ‘Sumo’. I have
tried to approach Leica to test this objective in a conventional SP5
microscope but the only unsatisfying answer I got from Leica was ‘the GSD
system is manufactured in a different unit from the confocals…’ and the GSD
people are, understandably, reluctant to let me remove it and try on our
confocal.

 

I had previous excellent experience with a 150x TIRF on a spinning disk
system. Has anyone tried the 160x on a confocal microscope? Any inputs?

 

Thanks !


Renato

 

Dr. Renato Arruda Mortara

Disciplina de Parasitologia

Escola Paulista de Medicina - UNIFESP

Rua Botucatu, 862 6o. andar

04023-062

São Paulo SP

Brasil

VOIP: 5576-4848 R: 2928

 <mailto:[hidden email]> [hidden email]

 <http://www.ecb.epm.br/~ramortara> www.ecb.epm.br/~ramortara

 

 

[Pedro Almada]

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The problem with Leica is they do some of the corrections outside of the
objective, on the tube lens. Not sure if the GSD has a specific tube lens
matching that objective as Leica is never forward with these things...

On 12 November 2014 10:51, Renato Mortara <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all
>
>
>
> Our institution has just acquired a GSD system from Leica that is based on
> a
> 160x 1.43 objective that sits in a ‘heavy’ stage they named ‘Sumo’. I have
> tried to approach Leica to test this objective in a conventional SP5
> microscope but the only unsatisfying answer I got from Leica was ‘the GSD
> system is manufactured in a different unit from the confocals…’ and the GSD
> people are, understandably, reluctant to let me remove it and try on our
> confocal.
>
>
>
> I had previous excellent experience with a 150x TIRF on a spinning disk
> system. Has anyone tried the 160x on a confocal microscope? Any inputs?
>
>
>
> Thanks !
>
>
> Renato
>
>
>
> Dr. Renato Arruda Mortara
>
> Disciplina de Parasitologia
>
> Escola Paulista de Medicina - UNIFESP
>
> Rua Botucatu, 862 6o. andar
>
> 04023-062
>
> São Paulo SP
>
> Brasil
>
> VOIP: 5576-4848 R: 2928
>
>  <mailto:[hidden email]> [hidden email]
>
>  <http://www.ecb.epm.br/~ramortara> www.ecb.epm.br/~ramortara
>
>
>
>
>