Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalI use the .17 PEN membrane slides for immunofluorescent labelling rather
frequently, without any problems, for what it's worth. I have noticed
that the PAP pen can dissolve/tear the membrane rather easily, so avoid
that. I typically give them a dip in ethanol then let them dry on a
slide-warmer for a few hours before microdissecting so that they're
completely dry, etc...
Peter
Dave Anchel wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Hi folks,
> I'm wondering if anyone out there has ever used the .17mm PEN membrane
> coverslips from PALM for immunofluorescence labelling prior to
> microdissection. The company sells two versions of the .17mm coverslips,
> one "4 sided sticky" and one "2 sided sticky". Are both versions stable under
> immunofluorescence labelling, or does slight lifting of the PEN membrane occur
> after the processing steps?
>
> Thanks,
> Dave Anchel,
> Toronto
>
>
>
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