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Upcoming microscopy course at Cold Spring Harbor Laboratory:
Quantitative Imaging: From Acquisition to Analysis
April 4 - 17, 2018
Application Deadline: January 31, 2018
Instructors:
Jennifer Waters, Harvard Medical School
Hunter Elliott, Harvard Medical School
Suliana Manley, EPFL, Switzerland
Talley Lambert, Harvard Medical School
Teaching Assistants: Anna Payne-Tobin Jost, Michael Weber, Marcelo Cicconet, Veronica Pessino & Jessica Hornick
Combining careful image acquisition with rigorous computational analysis allows extraction of quantitative data from light microscopy images that is far more informative and reproducible than what can be seen by eye. This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens, from tissues to cells to single molecules. The course is designed for quantitative cell and molecular biologists, biophysicists and bioengineers.
We provide a thorough treatment of the complete process of quantitative imaging, from the photons emitted from the sample to the extraction of biologically meaningful measurements from digital images. Material is covered in lectures, discussion groups and hands-on quantitative exercises using commercial microscopes and open-source image analysis tools.
Concepts Covered Include:
Widefield fluorescence microscopy
Laser scanning and spinning disk confocal microscopy
CCD, EM-CCD & sCMOS cameras
Total internal fluorescence microscopy (TIRF)
Light sheet microscopy
Super-resolution microscopy (structured illumination, STED & localization microscopy)
Imaging and analyzing ratiometric “biosensors” (including FRET)
Fluorescent proteins and live sample imaging
Image processing (filtering, de-noising, corrections, deconvolution)
Image segmentation
Quantitative shape and intensity measurements
Object detection and tracking
Machine learning
Designing and troubleshooting quantitative imaging experiments
...and more!
The course also includes a series of seminars highlighting applications of the methods we will discuss, including:
Suliana Manley, EPFL
Philipp Keller, Janelia Research Campus
Nathan Shaner, The Scintillon Institute
Florian Jug, MPI
Support & Stipends
-Major support provided by the National Cancer Institute
-We welcome submission of stipend requests with course applications. Stipends are available to offset tuition costs as follows:
US applicants (National Cancer Institute)
Interdisciplinary Fellowships (transitioning from outside biology) & Scholarships (transitioning from other biological disciplines) (Helmsley Charitable Trust)
International applicants (Howard Hughes Medical Institute)
To apply visit:
https://meetings.cshl.edu/courses.aspx?course=C-ECG&year=17
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