2D reconstruction
Locked
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello all: I am collecting in situ psfs from Arabdiopsis leaves.For me it is impossible to collect PSF's in z direction as I will also capture the leaf structure too and I only collect data in XY plane. I am wondering if it is possible to do 2D deconvolution by these collected PSF s with Huygens software(we haven't get the soft ware yet we would like to try this with the test licence of the software). I really appreciate any information and any idea. Sarah |
|
|
Hello Sarah,
Huygens as far as I know does not do any '2D' deconvolution, you might want to use one of the filters provided by Huygens though. For these it is not necessary to provide a PSF. The problem you experience with the contribution of the leave structure might be addressable by a spectral un-mixing approach. Cheers, jens
|
|
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I thought there was a wide field option that allows single image (or single optical slice if you like) deconvolution in huygens professionl. Never tried it though. Mariette ____________________________________________ Dr. M.P.C. Kemner-van de Corput, ____________________________________________ MGC - Dept. of Cell Biology & Genetics Erasmus Medical Center Dr. Molewaterplein 50, 3015 GE Rotterdam POB 2040, 3000 CA Rotterdam, The Netherlands Office: H-Ee751; tel: +31 10 704.3949 Lab: H-Ee710; tel lab: +31 10 704.3315 tel secr: +31 10 704.3169 ____________________________________________ http://www2.eur.nl/fgg/ch1/cellbiology/ http://www.thesis.kemner.biz/ ____________________________________________ Op Do, 13 maart, 2008 9:13 am, schreef Jens Rietdorf: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hello Sarah, > > Huygens as far as I know does not do any '2D' deconvolution, you might > want > to use one of the filters provided by Huygens though. For these it is not > necessary to provide a PSF. > > The problem you experience with the contribution of the leave structure > might be addressable by a spectral un-mixing approach. > > Cheers, jens > > > Sarah Kefayati wrote: >> >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> Hello all: >> >> I am collecting in situ psfs from Arabdiopsis leaves.For me it is >> impossible to collect PSF's in z direction as I will also capture the >> leaf structure too and I only collect data in XY plane. >> I am wondering if it is possible to do 2D deconvolution by these >> collected PSF s with Huygens software(we haven't get the soft ware yet >> we would like to try this with the test licence of the software). >> >> I really appreciate any information and any idea. >> >> Sarah >> >> > > -- > View this message in context: > http://www.nabble.com/2D-reconstruction-tp15995790p16023068.html > Sent from the Confocal Microscopy List mailing list archive at Nabble.com. > |
 
|
Kevin Ryan-2 |
Commercial response: 2D reconstruction
|
|
In reply to this post by Sarah Kefayati
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal AutoQuant is also an option, with measured and/or theoretic PSFs. Forwarded from our sales folks: >Dear Sarah, > >Deconvolution was something that originated in astronomy. 2D images and 2D PSFs were all >that were used. These approaches have been applied to light microscopy and have been >expanded to the 3D case to better improve the reassignment of out of focus blur. > >2D deconvolution with a 2D PSF is something that the AutoQuant deconvolution package is >capable of handling. The 2D deconvolution significantly improves an image's signal to >noise, contrast and resolution. With the AutoQuant product you can use a theoretical PSF >or measured PSF for deconvolution. > >Please visit http://www.mediacy.com/index.aspx?page=AutoDeblurVisualize to request a fully >functional 30 day trial of our software. > >Best wishes with your imaging. > >Jonathan Girroir >Software Product Manager > >Media Cybernetics >731 NE Olney Ct. #1 >Bend, OR 97701 >cell 518.588.6729 >office 541.383.4133 >fax 541.383.4133 Kevin Ryan - Media Cybernetics, Inc. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sarah Kefayati Sent: Tuesday, March 11, 2008 8:54 PM To: [hidden email] Subject: 2D reconstruction Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello all: I am collecting in situ psfs from Arabdiopsis leaves.For me it is impossible to collect PSF's in z direction as I will also capture the leaf structure too and I only collect data in XY plane. I am wondering if it is possible to do 2D deconvolution by these collected PSF s with Huygens software(we haven't get the soft ware yet we would like to try this with the test licence of the software). I really appreciate any information and any idea. Sarah |
|
|
In reply to this post by jens rietdorf
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Sarah, Check out the SVI website, www.svi.nl or contact them for information on their 2D deconvolution. there are some tips regarding setting the z-thickness, etc. a 2D is treated as a seriously truncated volume and will use a PSF. I would think that if the PSF is similarly truncated, then a calculated PSF might be more accurate. In case of the latter, you may need to measure the refractive index of your samples. Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] ************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ****** > > > Sarah Kefayati wrote: >> >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> Hello all: >> >> I am collecting in situ psfs from Arabdiopsis leaves.For me it is >> impossible to collect PSF's in z direction as I will also capture >> the >> leaf structure too and I only collect data in XY plane. >> I am wondering if it is possible to do 2D deconvolution by these >> collected PSF s with Huygens software(we haven't get the soft ware >> yet >> we would like to try this with the test licence of the software). >> >> I really appreciate any information and any idea. >> >> Sarah >> >> > > -- > View this message in context: http://www.nabble.com/2D- > reconstruction-tp15995790p16023068.html > Sent from the Confocal Microscopy List mailing list archive at > Nabble.com. |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |