2nd Annual Imaging in Research Course

[Jerry (Gerald) Sedgewick]

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Here's why the 2nd Annual Imaging in Research Course is being given in
August, 2011:

* Visual data in the form of images is often 50 percent of a
manuscript's content.  It's content is a reflection of the lab, and so
the quality and accuracy of images is as important as the written content.

* 44 percent of cases investigated by the Office of Research Integrity
in 2005-6 involved accusations of image fraud, compared with about 6
percent a decade before that (1). These cases are rising, and most
involve graduate and post-doctoral students.

* Students and staff who self-report familiarity with imaging programs
like Photoshop may be using it improperly.

In response to this, the "2nd Annual Imaging in Research Course: Ethics,
Acquisition, Post-Processing, Output and Quantization" is being held at
the Stadium Village Radisson Hotel on the University of Minnesota
campus, August 24 - 26, 2011.  Attendees can choose to attend the course
for 2- to 3-days.  This workshop will educate those in science, medicine
and engineering about correct techniques when acquiring,
post-processing, and adjusting images for outputs; along with techniques
that work for segmenting complex, biological images (for subsequent
image analysis).

Other benefits of taking the course will likely result in:

Faster acceptance of submitted manuscripts
Authors better able to demonstrate outcomes to their target audience
Faster results from quantitation, with improved ability to segment
desired features
Better documentation of imaging procedures
Standardization of post-processing
Learning to adjust and modify images minimally and through the objective
use of numbers.

Jerry Sedgewick will present.  Jerry directed a core light microscopy
and imaging facility for 15 years at the University of Minnesota,
published 2 books on Photoshop and digital imaging, and his quantitative
work has led to FDA approval for start up companies.

Please go to http://www.imagingandanalysis.com/seminars.html for more
information.  There is a limit of 12 seats.

The cost ranges from $390 for 2-days to $490 for 3-days. It includes
lunch, beverages and snacks.  Here's the summary for the days:

Day1: Ethics of digital imaging, sample preparation, calibration, best
acquisition practices on light microscopes; setting up Photoshop,
opening image stacks/12 bit images, rotate/crop,
De-colorizing/colorizing fluorescent samples, merging images,
colocalization, adjusting tones for 3D reconstructions, saving images,
resetting pixel resolution (resampling), creating automated steps
(macros). These functions also covered for the free programs GIMP and
Image J (when applicable).
Day2: Post Processing II:  uneven illumination correction, color
correction, histogram (tone) matching, correcting noise, scale bars,
extended focus, extended dynamic range, pseudocolor, tonal adjustment,
De-colorizing/colorizing fluorescent samples, merging images,
colocalization, adjusting tones for 3D reconstructions, saving images,
resetting pixel resolution (resampling), creating automated steps
(macros).  These functions also covered for the free programs GIMP and
Image J (when applicable).
Day 3: Segmenting in Photoshop for image analysis (quantization):
optical density and intensity measurements, creating binary files
through 3 methods, setting threshold at consistent value, unbiased
sampling (stereology), automating steps, measurement in image analysis
programs.

All the best,

Jerry Sedgewick