Dear all,
I am completely new to image analysis from confocal microscopy and I would like to seek advices about which softwares to use for 3D volume rendering and calculation of the volume after obtaining Z-stacks of immunostained Drosophila larvae brains. I briefly know that ImageJ (free) and Amira (expensive) are some of the available softwares, but can ImageJ perform 3D volume rendering and calculation?
I would greatly appreciate any advices given. Thank you!
Best regards,
Joan, PhD student
Singapore