488nm HeNe laser problem
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hello everyone in the forum
I opend this topic to get your opinions and experience about a trouble I got recently. Lately the HeNe 488nm emission laser (Melles-Griot) generates a striped image, with orizontal stripes of color alternated with dark ones, grossly like a venetiand blind, the bright difference is quite high. It happens at whatever setting I use (intensity, PMT gain, pixel dwell etc...) in standard mode as well as in spectral or DIC mode. After 15 minutes some of power on the laser starts working properly and the scan image is formed fine. Other lasers (408nm, 561nm, 633) work perfectly. The confocal microscopy set up is all branded Nikon. I kindly thank all of you who read this andwrote any hint or opinion. Best regards D.Dinucci PhD, University of Pisa - Italy |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Well, you sure have a problem - or a most remarkable laser :). But, seriously, whether it is your argon 488nm or your HeNe 633nm, what you are seeing is the typical symptom of a failing gas laser. The feedback system is attempting to maintain output, then reaches the maximum level of the anode current and trips out. It then cuts back in again .. and so on, giving you the 'venetian blinds'. I've seen it so many times ... It may be curable by re-alignment but most likely you need to have the laser re-gassed and rebuilt. The good news is that there are lots of companies offering competitive laser rebuilds these days, so shop around. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of griseum Sent: Tuesday, 5 July 2011 11:05 PM To: [hidden email] Subject: 488nm HeNe laser problem ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** hello everyone in the forum I opend this topic to get your opinions and experience about a trouble I got recently. Lately the HeNe 488nm emission laser (Melles-Griot) generates a striped image, with orizontal stripes of color alternated with dark ones, grossly like a venetiand blind, the bright difference is quite high. It happens at whatever setting I use (intensity, PMT gain, pixel dwell etc...) in standard mode as well as in spectral or DIC mode. After 15 minutes some of power on the laser starts working properly and the scan image is formed fine. Other lasers (408nm, 561nm, 633) work perfectly. The confocal microscopy set up is all branded Nikon. I kindly thank all of you who read this andwrote any hint or opinion. Best regards D.Dinucci PhD, University of Pisa - Italy -- View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/488nm-HeNe-laser-pr oblem-tp6549675p6549675.html Sent from the Confocal Microscopy List mailing list archive at Nabble.com. ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1388 / Virus Database: 1516/3744 - Release Date: 07/04/11 |
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Guy thank you so much for your brief but exhaustive explanation about the laser trouble. I myself did thought that the gas emission efficiency is not so performing anymore so the ionization can be reached later after a "buffer time" of energization of the remaining efficient gas. I do agree the laser is about to expire by comparing the working time so far and the halflife declared by the producer. Next, I take advantage of talking to you so if you can tell me more about the good news and the many companies that can compete with M-G with a good 488nm laser, I would be much more grateful to you. Best Regards Dino |
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In reply to this post by Guy Cox-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Maybe some of our Italian members could share their favourite laser service companies with Dino? I'm sure the laser can be overhauled - it shouldn't need replacing. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of griseum Sent: Wednesday, 6 July 2011 12:25 AM To: [hidden email] Subject: Re: 488nm HeNe laser problem ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Guy thank you so much for your brief but exhaustive explanation about the laser trouble. I myself did thought that the gas emission efficiency is not so performing anymore so the ionization can be reached later after a "buffer time" of energization of the remaining efficient gas. I do agree the laser is about to expire by comparing the working time so far and the halflife declared by the producer. Next, I take advantage of talking to you so if you can tell me more about the good news and the many companies that can compete with M-G with a good 488nm laser, I would be much more grateful to you. Best Regards Dino -- View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/488nm-HeNe-laser-pr oblem-tp6549675p6549957.html Sent from the Confocal Microscopy List mailing list archive at Nabble.com. ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1388 / Virus Database: 1516/3744 - Release Date: 07/04/11 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I've always used Dynamic Laser for refurbishing and repair, and I have been very happy with their service and prices. Contact: Dynamic Laser 2009 South 4130 West, Suite F Salt Lake City, Utah 84104 USA T. 801.952.9091 F. 801.952.9089 Email: [hidden email] http://www.dynamiclaser.net/index.php I find it worth the extra shipping cost to get this quality service, especially if you don't find anything else nearby. No financial interest, just a happy customer. Zoltan On Wed, Jul 6, 2011 at 1:21 AM, Guy Cox <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Maybe some of our Italian members could share their favourite laser > service companies with Dino? I'm sure the laser can be overhauled - it > shouldn't need replacing. > > Guy > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Australian Centre for Microscopy & Microanalysis, > Madsen Building F09, University of Sydney, NSW 2006 > > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of griseum > Sent: Wednesday, 6 July 2011 12:25 AM > To: [hidden email] > Subject: Re: 488nm HeNe laser problem > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Guy thank you so much for your brief but exhaustive explanation about > the > laser trouble. > I myself did thought that the gas emission efficiency is not so > performing > anymore so the ionization can be reached later after a "buffer time" of > energization of the remaining efficient gas. I do agree the laser is > about > to expire by comparing the working time so far and the halflife declared > by > the producer. > > Next, I take advantage of talking to you so if you can tell me more > about > the good news and the many companies that can compete with M-G with a > good > 488nm laser, I would be much more grateful to you. > > Best Regards > > Dino > > > -- > View this message in context: > http://confocal-microscopy-list.588098.n2.nabble.com/488nm-HeNe-laser-pr > oblem-tp6549675p6549957.html > Sent from the Confocal Microscopy List mailing list archive at > Nabble.com. > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 10.0.1388 / Virus Database: 1516/3744 - Release Date: 07/04/11 > -- Dr. Zoltan Cseresnyes Confocal and 2-Photon Microscopy Core Facility (MCF) Max-Delbrück-Center for Molecular Medicine Robert-Rössle-Str. 10 13125 Berlin |
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