A fun bit of eye candy

[Benjamin Smith]

*****
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I recently dredged up a confocal movie I made a while back to show how
confocal microscopes can be used to see inside objects, and figured it
would be fun to share.

This is a movie of a fairly stressed (likely due to the very high YFP load)
tobacco leaf pavement cell expressing cytoplasmic YFP.  The movie goes down
through the central vacuole, where you can see the transvacuolar strands
and finally the cell nucleus:
https://drive.google.com/file/d/1z5OIDkj6J5xXRNPDPBMkukeyeL8MfQyd/view?usp=sharing

Cheers,
   Ben Smith

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/

[Martin Wessendorf-5]

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*****

Dear Ben--

Cool!  --What software did you use to make it?

Martin Wessendorf

On Mon, Oct 5, 2020 at 5:44 PM Benjamin Smith <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I recently dredged up a confocal movie I made a while back to show how
> confocal microscopes can be used to see inside objects, and figured it
> would be fun to share.
>
> This is a movie of a fairly stressed (likely due to the very high YFP load)
> tobacco leaf pavement cell expressing cytoplasmic YFP.  The movie goes down
> through the central vacuole, where you can see the transvacuolar strands
> and finally the cell nucleus:
>
> https://drive.google.com/file/d/1z5OIDkj6J5xXRNPDPBMkukeyeL8MfQyd/view?usp=sharing
>
> Cheers,
>    Ben Smith
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
> Tel  [phone]
> Fax [phone]
> e-mail: [hidden email]
>
> https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/
>

[Benjamin Smith]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Matin.  This was just a quick and dirty movie made for fun with
Imaris about five years ago

On Tue, Oct 6, 2020 at 11:29 AM Martin Wessendorf <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Ben--
>
> Cool!  --What software did you use to make it?
>
> Martin Wessendorf
>
> On Mon, Oct 5, 2020 at 5:44 PM Benjamin Smith <[hidden email]
> >
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > I recently dredged up a confocal movie I made a while back to show how
> > confocal microscopes can be used to see inside objects, and figured it
> > would be fun to share.
> >
> > This is a movie of a fairly stressed (likely due to the very high YFP
> load)
> > tobacco leaf pavement cell expressing cytoplasmic YFP.  The movie goes
> down
> > through the central vacuole, where you can see the transvacuolar strands
> > and finally the cell nucleus:
> >
> >
> https://drive.google.com/file/d/1z5OIDkj6J5xXRNPDPBMkukeyeL8MfQyd/view?usp=sharing
> >
> > Cheers,
> >    Ben Smith
> >
> > --
> > Benjamin E. Smith, Ph. D.
> > Imaging Specialist, Vision Science
> > University of California, Berkeley
> > 195 Life Sciences Addition
> > Berkeley, CA  94720-3200
> > Tel  [phone]
> > Fax [phone]
> > e-mail: [hidden email]
> >
> >
> https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/
> >
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/

[Gary Laevsky]

*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Cool!  Reminds me of Fantastic Voyage.

Best,

Gary

> On Oct 6, 2020, at 3:13 PM, Benjamin Smith <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thanks Matin.  This was just a quick and dirty movie made for fun with
> Imaris about five years ago
>
>> On Tue, Oct 6, 2020 at 11:29 AM Martin Wessendorf <
>> [hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear Ben--
>>
>> Cool!  --What software did you use to make it?
>>
>> Martin Wessendorf
>>
>> On Mon, Oct 5, 2020 at 5:44 PM Benjamin Smith <[hidden email]
>>>
>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>> posting.
>>> *****
>>>
>>> I recently dredged up a confocal movie I made a while back to show how
>>> confocal microscopes can be used to see inside objects, and figured it
>>> would be fun to share.
>>>
>>> This is a movie of a fairly stressed (likely due to the very high YFP
>> load)
>>> tobacco leaf pavement cell expressing cytoplasmic YFP.  The movie goes
>> down
>>> through the central vacuole, where you can see the transvacuolar strands
>>> and finally the cell nucleus:
>>>
>>>
>> https://drive.google.com/file/d/1z5OIDkj6J5xXRNPDPBMkukeyeL8MfQyd/view?usp=sharing
>>>
>>> Cheers,
>>>   Ben Smith
>>>
>>> --
>>> Benjamin E. Smith, Ph. D.
>>> Imaging Specialist, Vision Science
>>> University of California, Berkeley
>>> 195 Life Sciences Addition
>>> Berkeley, CA  94720-3200
>>> Tel  [phone]
>>> Fax [phone]
>>> e-mail: [hidden email]
>>>
>>>
>> https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/
>>>
>>
>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
> Tel  [phone]
> Fax [phone]
> e-mail: [hidden email]
> https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/