A paper microscope?
Locked
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** For your sunday morning leisure reading... Sorry, I could not help but share news that someone has developed an origami-like microscope, made mostly of paper, that costs about 50 cents: https://www.youtube.com/watch?v=h8cF5QPPmWU https://www.youtube.com/watch?v=pBjIYB5Yk2I http://www.foldscope.com/ Apparently there are brightfield, darkfield, phase contrast, and even fluorescence versions available. And I ask, can one make a confocal version of this? Cheers, John Oreopoulos Staff Scientist Spectral Applied Research Inc. A Division of Andor Technology Richmond Hill, Ontario Canada www.spectral.ca |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thanks to John for sharing this innovative design. However, the video shows that only the "stand" and "stage" is made of paper. The LED illuminator and lens (?, not 100% sure) are definitely not. Regarding upgrading the paper scope -foldscope to the confocal, a major overhaul will be needed diverting from its current "flat" design. More perspective would be the smartphone based design. I was surprised by the quality of provided images. I have $1 microscope (from dollar store) with (3" tall) plastic body , plastic lenses (up to 40x), and reflecting mirror. Now I am intrigued to check if my plastic scope provides any useful image. Cheers, Arvydas Arvydas Matiukas, Ph.D. Director of Confocal&Two-Photon Imaging Core Facility Department of Neuroscience SUNY Upstate Medical University 766 Irving Ave., WH 3167 Syracuse, NY 13210 >>> John Oreopoulos 03/16/14 11:10 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** For your sunday morning leisure reading... Sorry, I could not help but share news that someone has developed an origami-like microscope, made mostly of paper, that costs about 50 cents: https://www.youtube.com/watch?v=h8cF5QPPmWU https://www.youtube.com/watch?v=pBjIYB5Yk2I http://www.foldscope.com/ Apparently there are brightfield, darkfield, phase contrast, and even fluorescence versions available. And I ask, can one make a confocal version of this? Cheers, John Oreopoulos Staff Scientist Spectral Applied Research Inc. A Division of Andor Technology Richmond Hill, Ontario Canada www.spectral.ca |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** John, This is really neat. Of course, it is essentially a paper copy of the original simple microscopes of Antoni van Leeuwenkoek--with LED illumination, and paper instead of brass for the supports. I recall that Robert Hooke described trying to use the simple microscope in the forward to Microscopiium, reporting that they were extremely hard on the eyes. Admittedly, the lenses that are used here may be better made, and the illumination of this one is much better. If it can actually be used either for education or for field work, it is a valuable addition. Here's a link to the paper. arxiv.org/pdf/1403.1211 Joel Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs On Sun, Mar 16, 2014 at 7:15 PM, Arvydas Matiukas <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Thanks to John for sharing this innovative design. However, the > video shows that only the "stand" and "stage" is made of paper. The LED > illuminator > and lens (?, not 100% sure) are definitely not. > > Regarding upgrading the paper scope -foldscope to the confocal, > a major overhaul will be needed diverting from its current "flat" > design. More perspective would be the smartphone based design. > > I was surprised by the quality of provided images. I have $1 microscope > (from dollar store) with (3" tall) plastic body , plastic lenses (up to > 40x), > and reflecting mirror. Now I am intrigued to check if my plastic scope > provides any useful image. > > > > Cheers, > Arvydas > > > > Arvydas Matiukas, Ph.D. > Director of Confocal&Two-Photon Imaging Core Facility > Department of Neuroscience > SUNY Upstate Medical University > 766 Irving Ave., WH 3167 > Syracuse, NY 13210 > > >>> John Oreopoulos 03/16/14 11:10 AM >>> > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > For your sunday morning leisure reading... Sorry, I could not help but > share news that someone has developed an origami-like microscope, made > mostly of paper, that costs about 50 cents: > > https://www.youtube.com/watch?v=h8cF5QPPmWU > https://www.youtube.com/watch?v=pBjIYB5Yk2I > > http://www.foldscope.com/ > > Apparently there are brightfield, darkfield, phase contrast, and even > fluorescence versions available. And I ask, can one make a confocal version > of this? > > Cheers, > > > John Oreopoulos > Staff Scientist > Spectral Applied Research Inc. > A Division of Andor Technology > Richmond Hill, Ontario > Canada > www.spectral.ca > |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Whoops - typo - van Leeuwenhoek, not van Leeuwenkoek. mea culpa Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs On Sun, Mar 16, 2014 at 9:32 PM, JOEL B. SHEFFIELD <[hidden email]> wrote: > John, > > This is really neat. Of course, it is essentially a paper copy of the > original simple microscopes of Antoni van Leeuwenkoek--with LED > illumination, and paper instead of brass for the supports. I recall that > Robert Hooke described trying to use the simple microscope in the forward > to Microscopiium, reporting that they were extremely hard on the eyes. > Admittedly, the lenses that are used here may be better made, and the > illumination of this one is much better. If it can actually be used either > for education or for field work, it is a valuable addition. > > Here's a link to the paper. > arxiv.org/pdf/1403.1211 > > Joel > > > > Joel B. Sheffield, Ph.D > Department of Biology > Temple University > Philadelphia, PA 19122 > Voice: 215 204 8839 > e-mail: [hidden email] > URL: http://astro.temple.edu/~jbs > > > On Sun, Mar 16, 2014 at 7:15 PM, Arvydas Matiukas <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Thanks to John for sharing this innovative design. However, the >> video shows that only the "stand" and "stage" is made of paper. The LED >> illuminator >> and lens (?, not 100% sure) are definitely not. >> >> Regarding upgrading the paper scope -foldscope to the confocal, >> a major overhaul will be needed diverting from its current "flat" >> design. More perspective would be the smartphone based design. >> >> I was surprised by the quality of provided images. I have $1 microscope >> (from dollar store) with (3" tall) plastic body , plastic lenses (up to >> 40x), >> and reflecting mirror. Now I am intrigued to check if my plastic scope >> provides any useful image. >> >> >> >> Cheers, >> Arvydas >> >> >> >> Arvydas Matiukas, Ph.D. >> Director of Confocal&Two-Photon Imaging Core Facility >> Department of Neuroscience >> SUNY Upstate Medical University >> 766 Irving Ave., WH 3167 >> Syracuse, NY 13210 >> >> >>> John Oreopoulos 03/16/14 11:10 AM >>> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> For your sunday morning leisure reading... Sorry, I could not help but >> share news that someone has developed an origami-like microscope, made >> mostly of paper, that costs about 50 cents: >> >> https://www.youtube.com/watch?v=h8cF5QPPmWU >> https://www.youtube.com/watch?v=pBjIYB5Yk2I >> >> http://www.foldscope.com/ >> >> Apparently there are brightfield, darkfield, phase contrast, and even >> fluorescence versions available. And I ask, can one make a confocal version >> of this? >> >> Cheers, >> >> >> John Oreopoulos >> Staff Scientist >> Spectral Applied Research Inc. >> A Division of Andor Technology >> Richmond Hill, Ontario >> Canada >> www.spectral.ca >> > > |
|
|
In reply to this post by JOEL B. SHEFFIELD
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** As an aside to this topic, I may mention that with a bit of 'glass-work' on a Sunday afternoon you can create the spherical ball lens needed for Leeuwenhoek (-type) microscope from glass pasteur pipette. This was shown to us by Shinya Inoue and Tomomi Tani at MBL. First, you need to soften the tip of the glass pasteur pipette on bunsen burner and stretch it into thin wire. Then you hold this wire on top of the flame and let the molten glass roll into a sphere. It does take some practice to make sure that you do not trap air bubbles in the resulting spherical ball lens. But, not that difficult. Then, you punch holes in two sturdy pieces of plastic that are just a bit smaller than the diameter of sphere. Put the sphere between the pieces of plastic and glue the plastic parts together. This is much like Leeuwenhoek microscope. If you put a drop of water with some critters on one side of ball lens, you can see them magnified on the other side by bringing the lens close to the eye. We distributed some of these during an open day and they turned out to be a hit. Shalin Assistant Research Scientist, Marine Biological Laboratory, 7 MBL Street, Woods Hole MA 02543, USA website: http://mshalin.com (office) Lillie 110, (ph) 508-289-7374. On Sun, Mar 16, 2014 at 9:32 PM, JOEL B. SHEFFIELD <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > John, > > This is really neat. Of course, it is essentially a paper copy of the > original simple microscopes of Antoni van Leeuwenkoek--with LED > illumination, and paper instead of brass for the supports. I recall that > Robert Hooke described trying to use the simple microscope in the forward > to Microscopiium, reporting that they were extremely hard on the eyes. > Admittedly, the lenses that are used here may be better made, and the > illumination of this one is much better. If it can actually be used either > for education or for field work, it is a valuable addition. > > Here's a link to the paper. > arxiv.org/pdf/1403.1211 > > Joel > > > > Joel B. Sheffield, Ph.D > Department of Biology > Temple University > Philadelphia, PA 19122 > Voice: 215 204 8839 > e-mail: [hidden email] > URL: http://astro.temple.edu/~jbs > > > On Sun, Mar 16, 2014 at 7:15 PM, Arvydas Matiukas <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Thanks to John for sharing this innovative design. However, the >> video shows that only the "stand" and "stage" is made of paper. The LED >> illuminator >> and lens (?, not 100% sure) are definitely not. >> >> Regarding upgrading the paper scope -foldscope to the confocal, >> a major overhaul will be needed diverting from its current "flat" >> design. More perspective would be the smartphone based design. >> >> I was surprised by the quality of provided images. I have $1 microscope >> (from dollar store) with (3" tall) plastic body , plastic lenses (up to >> 40x), >> and reflecting mirror. Now I am intrigued to check if my plastic scope >> provides any useful image. >> >> >> >> Cheers, >> Arvydas >> >> >> >> Arvydas Matiukas, Ph.D. >> Director of Confocal&Two-Photon Imaging Core Facility >> Department of Neuroscience >> SUNY Upstate Medical University >> 766 Irving Ave., WH 3167 >> Syracuse, NY 13210 >> >> >>> John Oreopoulos 03/16/14 11:10 AM >>> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> For your sunday morning leisure reading... Sorry, I could not help but >> share news that someone has developed an origami-like microscope, made >> mostly of paper, that costs about 50 cents: >> >> https://www.youtube.com/watch?v=h8cF5QPPmWU >> https://www.youtube.com/watch?v=pBjIYB5Yk2I >> >> http://www.foldscope.com/ >> >> Apparently there are brightfield, darkfield, phase contrast, and even >> fluorescence versions available. And I ask, can one make a confocal version >> of this? >> >> Cheers, >> >> >> John Oreopoulos >> Staff Scientist >> Spectral Applied Research Inc. >> A Division of Andor Technology >> Richmond Hill, Ontario >> Canada >> www.spectral.ca >> |
|
|
In reply to this post by John Oreopoulos
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Am 16.03.2014 16:07, schrieb John Oreopoulos: > Apparently there are brightfield, darkfield, phase contrast, and even fluorescence versions available. And I ask, can one make a confocal version of this? Well, this is a simple microscope, not a compound one. So there is no intermediate image and thus no plane where one could place the detection pinhole. Therefore, the short answer is no, you can't make a confocal version. Of course, one could try to redesign the whole thing. One would probably need a more stable stand to ensure a fixed tube length. Or, use infinity optics. What to use for detector? Maybe a smartphone camera in a spinning disk version? I guess in any case we would move far far away from the <1$ goal. Still might be an interesting and fun-project to try... Here are some designs to try, from the 1960ies, so the patent is expired. One is with Nipkow-disks, another with double-sided mirror which is used for scanning and descanning. https://commons.wikimedia.org/wiki/File:KlausWeber-USPatent3518014-Fig7.png (see also other images linked on the page) I guess the images are sort of self-explanatory to the confocalist. For an English description, you have to click on the linked patent application, a short German description can be found here: https://de.wikipedia.org/wiki/Konfokalmikroskop#1966:_.E2.80.9EVorrichtung_zur_optischen_Abtastung_mikroskopischer_Objekte.E2.80.9C The fun part would be to figure out how to build a confocal really cheap. Let's not think about whether it would be worth it, that'd probably spoil the whole approach... Cheers, Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I'm sure I remember a paper compound microscope from many years ago. Obviously no LED illuminator. I think you squeezed a bellows-folded section to focus. Anyone else remember this, or am I the last left standing? Guy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steffen Dietzel Sent: Monday, 17 March 2014 8:58 PM To: [hidden email] Subject: Re: A paper microscope? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Am 16.03.2014 16:07, schrieb John Oreopoulos: > Apparently there are brightfield, darkfield, phase contrast, and even fluorescence versions available. And I ask, can one make a confocal version of this? Well, this is a simple microscope, not a compound one. So there is no intermediate image and thus no plane where one could place the detection pinhole. Therefore, the short answer is no, you can't make a confocal version. Of course, one could try to redesign the whole thing. One would probably need a more stable stand to ensure a fixed tube length. Or, use infinity optics. What to use for detector? Maybe a smartphone camera in a spinning disk version? I guess in any case we would move far far away from the <1$ goal. Still might be an interesting and fun-project to try... Here are some designs to try, from the 1960ies, so the patent is expired. One is with Nipkow-disks, another with double-sided mirror which is used for scanning and descanning. https://commons.wikimedia.org/wiki/File:KlausWeber-USPatent3518014-Fig7.png (see also other images linked on the page) I guess the images are sort of self-explanatory to the confocalist. For an English description, you have to click on the linked patent application, a short German description can be found here: https://de.wikipedia.org/wiki/Konfokalmikroskop#1966:_.E2.80.9EVorrichtung_zur_optischen_Abtastung_mikroskopischer_Objekte.E2.80.9C The fun part would be to figure out how to build a confocal really cheap. Let's not think about whether it would be worth it, that'd probably spoil the whole approach... Cheers, Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Steffen Dietzl mentioned this a while ago in a lab class discussion: "There used to be a paper microscope with plastic lenses on the German market, for about 10-15 Euro (ISBN 3935364083) but the assembly took a little long for the course (4-6 h), so I dropped that. It did deliver a surprisingly good quality though." That was the first time I read about paper microscopes. With the same motivation as Manu Prakash, a nice paper here, using the halogen lamp for fluorescence: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031638 and surely also relevant for a minimal-cost microscopy approach (but on the other side of the eyepiece): http://www.malariajournal.com/content/11/1/352 _____________________________________ Philippe Laissue, PhD, Bioimaging Manager School of Biological Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK (0044) 01206 872246 / (0044) 07842 676 456 [hidden email] privatewww.essex.ac.uk/~plaissue On 17 March 2014 12:43, Guy Cox <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I'm sure I remember a paper compound microscope from many years ago. > Obviously no LED illuminator. I think you squeezed a bellows-folded > section to focus. Anyone else remember this, or am I the last left > standing? > > Guy > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Steffen Dietzel > Sent: Monday, 17 March 2014 8:58 PM > To: [hidden email] > Subject: Re: A paper microscope? > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Am 16.03.2014 16:07, schrieb John Oreopoulos: > > Apparently there are brightfield, darkfield, phase contrast, and even > fluorescence versions available. And I ask, can one make a confocal version > of this? > > Well, this is a simple microscope, not a compound one. So there is no > intermediate image and thus no plane where one could place the detection > pinhole. Therefore, the short answer is no, you can't make a confocal > version. > > Of course, one could try to redesign the whole thing. One would probably > need a more stable stand to ensure a fixed tube length. Or, use infinity > optics. What to use for detector? Maybe a smartphone camera in a > spinning disk version? I guess in any case we would move far far away > from the <1$ goal. Still might be an interesting and fun-project to try... > > Here are some designs to try, from the 1960ies, so the patent is > expired. One is with Nipkow-disks, another with double-sided mirror > which is used for scanning and descanning. > https://commons.wikimedia.org/wiki/File:KlausWeber-USPatent3518014-Fig7.png > (see also other images linked on the page) > > I guess the images are sort of self-explanatory to the confocalist. For > an English description, you have to click on the linked patent > application, a short German description can be found here: > > https://de.wikipedia.org/wiki/Konfokalmikroskop#1966:_.E2.80.9EVorrichtung_zur_optischen_Abtastung_mikroskopischer_Objekte.E2.80.9C > > The fun part would be to figure out how to build a confocal really > cheap. Let's not think about whether it would be worth it, that'd > probably spoil the whole approach... > > Cheers, > > Steffen > > > > -- > ------------------------------------------------------------ > Steffen Dietzel, PD Dr. rer. nat > Ludwig-Maximilians-Universität München > Walter-Brendel-Zentrum für experimentelle Medizin (WBex) > Head of light microscopy > > Mail room: > Marchioninistr. 15, D-81377 München > > Building location: > Marchioninistr. 27, München-Großhadern > |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |