A question about splitting a TiSa laser beam and controlling its power
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yuansheng sun |
A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Listers, I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at 800 nm). I know some people did this way to feed one TiSa laser to two microscopes. I am wondering if I can get a simple beam splitter for this purpose. And also, what would be the simple (and reliable) device you would suggest to control the TiSa laser power? Thanks a lot in advance for any suggestion. Best regards, Yuansheng Sun, Keck Center for Cellular Imaging University of Virginia |
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Arvydas Matiukas |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Yuansheng, At our confocal Core we split Coherent Chameleon laser between LSM510 NLO and Thorlabs 2p confocal. Thorlabs installed the splitter and and beam power controller. The primary laser control still is either manual of through Zeiss LSM software. We purchased everything together so you should contact Thorlabs about the splitter and power controller pricing. I assume it is (slightly?) below $10k. Best, Arvydas ------------------------------------------ Arvydas Matiukas, Ph.D. Director of Confocal&Two-Photon Imaging Core Facility Department of Pharmacology SUNY Upstate Medical University 766 Irving Ave., WH 3167 Syracuse, NY 13210 tel.: 315-464-7997 fax: 315-464-8014 email: [hidden email] >>> yuansheng sun 01/29/13 1:41 PM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Listers, I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at 800 nm). I know some people did this way to feed one TiSa laser to two microscopes. I am wondering if I can get a simple beam splitter for this purpose. And also, what would be the simple (and reliable) device you would suggest to control the TiSa laser power? Thanks a lot in advance for any suggestion. Best regards, Yuansheng Sun, Keck Center for Cellular Imaging University of Virginia |
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Craig Brideau |
Re: A question about splitting a TiSa laser beam and controlling its power
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In reply to this post by yuansheng sun
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The prism splits the laser into two beams, with the amount of power going into each beam governed by the angle of the waveplate. Note you may need another waveplate to rotate the polarization of one of the beams back into your microscope. Craig On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Listers, > > I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at 800 > nm). I know some people did this way to feed one TiSa laser to two > microscopes. I am wondering if I can get a simple beam splitter for this > purpose. And also, what would be the simple (and reliable) device you > would suggest to control the TiSa laser power? Thanks a lot in advance for > any suggestion. > > Best regards, > Yuansheng Sun, > Keck Center for Cellular Imaging > University of Virginia > |
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Johannes Helm |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Good evening, Craig has already provided the answer, see below. I just would like to mention in addition that one can buy lambda/2 plates, which show the lambda/2 effect with minor deviations, only, i.e.: are achromatic, over the entire wavelength range of the usual approx. 680nm - approx. 1060nm Ti:Sap lasers common in non linear laser scanning microscopy (and have AR coatings for that entire wavelength range). They are possibly somewhat more expensive than lambda/2 plates but may nevertheless be the item of choice. I do not have ANY commercial affiliations and, hence, allow myself to mention that I personally have experienced that the achromatic lambda/2 plates from Halle are excellent units for the purpose, www.b-halle.de . Of course, one also will need a suitable polarization beam splitter and the opto mechanics to position and adjust both, the lambda/2 plate and the polarization beam splitter in the appropriate way. Glan prisms, as Craig mentioned, will be excellent for the purpose but they usually will emit not one but one main and one minor deflected beam under an angle different from 90 degrees, while conventional polarization beam splitters will emit the deflected beam at roughly 90 degrees. Also, check out before buying any beam splitter about potential "un-flatnesses" on the lateral surfaces of the Glan prism or Polarization beam splitter. A Foster prism might also be a good item if you want one beam to continue straight forward and the other to continue at 45 degrees or so. Also, if you want to install that lambda/2 plate on an optical table with a lot of other elements like EOMs, photodiodes, spatial filters, beam expanders and the like and if all will be "hidden" behind a metal curtain to protect laser radiation from becoming dangerous in the lab, then you should consider to install the lambda/2 plate in a motor driven rotator, which you can control from a PC. Best wishes, Johannes > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The > prism splits the laser into two beams, with the amount of power going into > each beam governed by the angle of the waveplate. Note you may need > another waveplate to rotate the polarization of one of the beams back into > your microscope. > > Craig > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear Listers, >> >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at >> 800 >> nm). I know some people did this way to feed one TiSa laser to two >> microscopes. I am wondering if I can get a simple beam splitter for >> this >> purpose. And also, what would be the simple (and reliable) device you >> would suggest to control the TiSa laser power? Thanks a lot in advance >> for >> any suggestion. >> >> Best regards, >> Yuansheng Sun, >> Keck Center for Cellular Imaging >> University of Virginia >> > -- P. Johannes Helm Voice: (+47) 228 51159 (office) Fax: (+47) 228 51499 (office) |
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Craig Brideau |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** As Johannes says, you need optomechanics to rotate the waveplate. You can either use a manual rotation mount (you don't need very fine control, so a thumb-wheel is fine), or if you are adverse to sticking your hand anywhere near the beam you can use a motorized mount. In systems with other safety mechanisms, (i.e. laser blocks and such) I have used manual mounts. In situations where it will be used by inexperienced users I tend to motorize things to keep untrained hands away from the laser and optics. Craig On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Good evening, > > Craig has already provided the answer, see below. > > I just would like to mention in addition that one can buy lambda/2 plates, > which show the lambda/2 effect with minor deviations, only, i.e.: are > achromatic, over the entire wavelength range of the usual approx. 680nm - > approx. 1060nm Ti:Sap lasers common in non linear laser scanning > microscopy (and have AR coatings for that entire wavelength range). They > are possibly somewhat more expensive than lambda/2 plates but may > nevertheless be the item of choice. I do not have ANY commercial > affiliations and, hence, allow myself to mention that I personally have > experienced that the achromatic lambda/2 plates from Halle are excellent > units for the purpose, www.b-halle.de . > > Of course, one also will need a suitable polarization beam splitter and > the opto mechanics to position and adjust both, the lambda/2 plate and the > polarization beam splitter in the appropriate way. Glan prisms, as Craig > mentioned, will be excellent for the purpose but they usually will emit > not one but one main and one minor deflected beam under an angle different > from 90 degrees, while conventional polarization beam splitters will emit > the deflected beam at roughly 90 degrees. Also, check out before buying > any beam splitter about potential "un-flatnesses" on the lateral surfaces > of the Glan prism or Polarization beam splitter. A Foster prism might also > be a good item if you want one beam to continue straight forward and the > other to continue at 45 degrees or so. > > > > Also, if you want to install that lambda/2 plate on an optical table with > a lot of other elements like EOMs, photodiodes, spatial filters, beam > expanders and the like and if all will be "hidden" behind a metal curtain > to protect laser radiation from becoming dangerous in the lab, then you > should consider to install the lambda/2 plate in a motor driven rotator, > which you can control from a PC. > > Best wishes, > > Johannes > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The > > prism splits the laser into two beams, with the amount of power going > into > > each beam governed by the angle of the waveplate. Note you may need > > another waveplate to rotate the polarization of one of the beams back > into > > your microscope. > > > > Craig > > > > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > > <[hidden email]>wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> Dear Listers, > >> > >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at > >> 800 > >> nm). I know some people did this way to feed one TiSa laser to two > >> microscopes. I am wondering if I can get a simple beam splitter for > >> this > >> purpose. And also, what would be the simple (and reliable) device you > >> would suggest to control the TiSa laser power? Thanks a lot in advance > >> for > >> any suggestion. > >> > >> Best regards, > >> Yuansheng Sun, > >> Keck Center for Cellular Imaging > >> University of Virginia > >> > > > > > -- > P. Johannes Helm > > Voice: (+47) 228 51159 (office) > Fax: (+47) 228 51499 (office) > |
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Armstrong, Brian |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, yes, and for a rotation mount we used: Newport RSP-IT (http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no competing interest]. We can adjust the percent power sent to each microscope this way. (The beam then goes through an AOM which adjusts the percent remaining power to the microscope, [so we have two AOMs]). Cheers, Brian D Armstrong PhD Assistant Research Professor Director, Light Microscopy Core Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Tuesday, January 29, 2013 1:19 PM To: [hidden email] Subject: Re: A question about splitting a TiSa laser beam and controlling its power ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** As Johannes says, you need optomechanics to rotate the waveplate. You can either use a manual rotation mount (you don't need very fine control, so a thumb-wheel is fine), or if you are adverse to sticking your hand anywhere near the beam you can use a motorized mount. In systems with other safety mechanisms, (i.e. laser blocks and such) I have used manual mounts. In situations where it will be used by inexperienced users I tend to motorize things to keep untrained hands away from the laser and optics. Craig On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Good evening, > > Craig has already provided the answer, see below. > > I just would like to mention in addition that one can buy lambda/2 plates, > which show the lambda/2 effect with minor deviations, only, i.e.: are > achromatic, over the entire wavelength range of the usual approx. 680nm - > approx. 1060nm Ti:Sap lasers common in non linear laser scanning > microscopy (and have AR coatings for that entire wavelength range). They > are possibly somewhat more expensive than lambda/2 plates but may > nevertheless be the item of choice. I do not have ANY commercial > affiliations and, hence, allow myself to mention that I personally have > experienced that the achromatic lambda/2 plates from Halle are excellent > units for the purpose, www.b-halle.de . > > Of course, one also will need a suitable polarization beam splitter and > the opto mechanics to position and adjust both, the lambda/2 plate and the > polarization beam splitter in the appropriate way. Glan prisms, as Craig > mentioned, will be excellent for the purpose but they usually will emit > not one but one main and one minor deflected beam under an angle different > from 90 degrees, while conventional polarization beam splitters will emit > the deflected beam at roughly 90 degrees. Also, check out before buying > any beam splitter about potential "un-flatnesses" on the lateral surfaces > of the Glan prism or Polarization beam splitter. A Foster prism might also > be a good item if you want one beam to continue straight forward and the > other to continue at 45 degrees or so. > > > > Also, if you want to install that lambda/2 plate on an optical table with > a lot of other elements like EOMs, photodiodes, spatial filters, beam > expanders and the like and if all will be "hidden" behind a metal curtain > to protect laser radiation from becoming dangerous in the lab, then you > should consider to install the lambda/2 plate in a motor driven rotator, > which you can control from a PC. > > Best wishes, > > Johannes > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The > > prism splits the laser into two beams, with the amount of power going > into > > each beam governed by the angle of the waveplate. Note you may need > > another waveplate to rotate the polarization of one of the beams back > into > > your microscope. > > > > Craig > > > > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > > <[hidden email]>wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> Dear Listers, > >> > >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at > >> 800 > >> nm). I know some people did this way to feed one TiSa laser to two > >> microscopes. I am wondering if I can get a simple beam splitter for > >> this > >> purpose. And also, what would be the simple (and reliable) device you > >> would suggest to control the TiSa laser power? Thanks a lot in advance > >> for > >> any suggestion. > >> > >> Best regards, > >> Yuansheng Sun, > >> Keck Center for Cellular Imaging > >> University of Virginia > >> > > > > > -- > P. Johannes Helm > > Voice: (+47) 228 51159 (office) > Fax: (+47) 228 51499 (office) > --------------------------------------------------------------------- *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) --------------------------------------------------------------------- |
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yuansheng sun |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you all so much for the great suggestions. Following the suggested direction, I searched and found an application note from Newport (no competing interest). There are two beams out of the Glan-Laser prism - one is the main beam output and the other is called the beam dump. I am wondering if I can achieve any split between the main output and the dump by varying the degree of the half wave plate. I assume the sum of the main output power and the dump power is close to the Ti:Sa laser power (~1.3 W for our system at 800 nm). Brian, can you share your AOM information? Thanks. Sheng On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, yes, and for a rotation mount we used: Newport RSP-IT ( > http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no > competing interest]. > We can adjust the percent power sent to each microscope this way. (The > beam then goes through an AOM which adjusts the percent remaining power to > the microscope, [so we have two AOMs]). > > Cheers, > > Brian D Armstrong PhD > Assistant Research Professor > Director, Light Microscopy Core > Beckman Research Institute > City of Hope > Dept of Neuroscience > 1450 E Duarte Rd > Duarte, CA 91010 > 626-256-4673 x62872 > > > http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Craig Brideau > Sent: Tuesday, January 29, 2013 1:19 PM > To: [hidden email] > Subject: Re: A question about splitting a TiSa laser beam and controlling > its power > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > As Johannes says, you need optomechanics to rotate the waveplate. You can > either use a manual rotation mount (you don't need very fine control, so a > thumb-wheel is fine), or if you are adverse to sticking your hand anywhere > near the beam you can use a motorized mount. In systems with other safety > mechanisms, (i.e. laser blocks and such) I have used manual mounts. In > situations where it will be used by inexperienced users I tend to motorize > things to keep untrained hands away from the laser and optics. > > Craig > > > On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] > >wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Good evening, > > > > Craig has already provided the answer, see below. > > > > I just would like to mention in addition that one can buy lambda/2 > plates, > > which show the lambda/2 effect with minor deviations, only, i.e.: are > > achromatic, over the entire wavelength range of the usual approx. 680nm - > > approx. 1060nm Ti:Sap lasers common in non linear laser scanning > > microscopy (and have AR coatings for that entire wavelength range). They > > are possibly somewhat more expensive than lambda/2 plates but may > > nevertheless be the item of choice. I do not have ANY commercial > > affiliations and, hence, allow myself to mention that I personally have > > experienced that the achromatic lambda/2 plates from Halle are excellent > > units for the purpose, www.b-halle.de . > > > > Of course, one also will need a suitable polarization beam splitter and > > the opto mechanics to position and adjust both, the lambda/2 plate and > the > > polarization beam splitter in the appropriate way. Glan prisms, as Craig > > mentioned, will be excellent for the purpose but they usually will emit > > not one but one main and one minor deflected beam under an angle > different > > from 90 degrees, while conventional polarization beam splitters will emit > > the deflected beam at roughly 90 degrees. Also, check out before buying > > any beam splitter about potential "un-flatnesses" on the lateral surfaces > > of the Glan prism or Polarization beam splitter. A Foster prism might > also > > be a good item if you want one beam to continue straight forward and the > > other to continue at 45 degrees or so. > > > > > > > > Also, if you want to install that lambda/2 plate on an optical table with > > a lot of other elements like EOMs, photodiodes, spatial filters, beam > > expanders and the like and if all will be "hidden" behind a metal curtain > > to protect laser radiation from becoming dangerous in the lab, then you > > should consider to install the lambda/2 plate in a motor driven rotator, > > which you can control from a PC. > > > > Best wishes, > > > > Johannes > > > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The > > > prism splits the laser into two beams, with the amount of power going > > into > > > each beam governed by the angle of the waveplate. Note you may need > > > another waveplate to rotate the polarization of one of the beams back > > into > > > your microscope. > > > > > > Craig > > > > > > > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > > > <[hidden email]>wrote: > > > > > >> ***** > > >> To join, leave or search the confocal microscopy listserv, go to: > > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > >> ***** > > >> > > >> Dear Listers, > > >> > > >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at > > >> 800 > > >> nm). I know some people did this way to feed one TiSa laser to two > > >> microscopes. I am wondering if I can get a simple beam splitter for > > >> this > > >> purpose. And also, what would be the simple (and reliable) device you > > >> would suggest to control the TiSa laser power? Thanks a lot in > advance > > >> for > > >> any suggestion. > > >> > > >> Best regards, > > >> Yuansheng Sun, > > >> Keck Center for Cellular Imaging > > >> University of Virginia > > >> > > > > > > > > > -- > > P. Johannes Helm > > > > Voice: (+47) 228 51159 (office) > > Fax: (+47) 228 51499 (office) > > > > > --------------------------------------------------------------------- > *SECURITY/CONFIDENTIALITY WARNING: > This message and any attachments are intended solely for the individual or > entity to which they are addressed. This communication may contain > information that is privileged, confidential, or exempt from disclosure > under applicable law (e.g., personal health information, research data, > financial information). Because this e-mail has been sent without > encryption, individuals other than the intended recipient may be able to > view the information, forward it to others or tamper with the information > without the knowledge or consent of the sender. If you are not the intended > recipient, or the employee or person responsible for delivering the message > to the intended recipient, any dissemination, distribution or copying of > the communication is strictly prohibited. If you received the communication > in error, please notify the sender immediately by replying to this message > and deleting the message and any accompanying files from your system. If, > due to the security risks, you do not wish to receive further > communications via e-mail, please reply to this message and inform the > sender that you do not wish to receive further e-mail from the sender. > (fpc5p) > --------------------------------------------------------------------- > |
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Craig Brideau |
Re: A question about splitting a TiSa laser beam and controlling its power
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Exactly. The Glan-laser splits the input beam into two output beams. One output is horizontally polarized, while the other is vertical. The amount of power in each output depends on the polarization angle of the input beam. You use a half-waveplate to rotate the input beam polarization to adjust the power between the two beams. Note that the two outputs will always add up to the power of the single input; you are splitting the power of the input beam between the two outputs. You can then use a second waveplate and Glan-laser to adjust the power of one of the two output arms by splitting it again. The output of the second cube should be split between a metal beam dump and your second microscope. The main advantage of this technique is it allows for smooth analog control of the power. It is fairly slow since you have to spin a waveplate. If you need higher speed you can use a pockel's cell instead, but these are even more expensive than the Glan-laser/waveplate combination. Craig On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Thank you all so much for the great suggestions. Following the > suggested direction, I searched and found an application note from Newport > (no competing interest). There are two beams out of the Glan-Laser prism - > one is the main beam output and the other is called the beam dump. I am > wondering if I can achieve any split between the main output and the dump > by varying the degree of the half wave plate. I assume the sum of the main > output power and the dump power is close to the Ti:Sa laser power (~1.3 W > for our system at 800 nm). > > Brian, can you share your AOM information? Thanks. > > Sheng > > On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email] > >wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi, yes, and for a rotation mount we used: Newport RSP-IT ( > > http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no > > competing interest]. > > We can adjust the percent power sent to each microscope this way. (The > > beam then goes through an AOM which adjusts the percent remaining power > to > > the microscope, [so we have two AOMs]). > > > > Cheers, > > > > Brian D Armstrong PhD > > Assistant Research Professor > > Director, Light Microscopy Core > > Beckman Research Institute > > City of Hope > > Dept of Neuroscience > > 1450 E Duarte Rd > > Duarte, CA 91010 > > 626-256-4673 x62872 > > > > > > > http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx > > > > > > -----Original Message----- > > From: Confocal Microscopy List [mailto:[hidden email]] > > On Behalf Of Craig Brideau > > Sent: Tuesday, January 29, 2013 1:19 PM > > To: [hidden email] > > Subject: Re: A question about splitting a TiSa laser beam and controlling > > its power > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > As Johannes says, you need optomechanics to rotate the waveplate. You > can > > either use a manual rotation mount (you don't need very fine control, so > a > > thumb-wheel is fine), or if you are adverse to sticking your hand > anywhere > > near the beam you can use a motorized mount. In systems with other > safety > > mechanisms, (i.e. laser blocks and such) I have used manual mounts. In > > situations where it will be used by inexperienced users I tend to > motorize > > things to keep untrained hands away from the laser and optics. > > > > Craig > > > > > > On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] > > >wrote: > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > Good evening, > > > > > > Craig has already provided the answer, see below. > > > > > > I just would like to mention in addition that one can buy lambda/2 > > plates, > > > which show the lambda/2 effect with minor deviations, only, i.e.: are > > > achromatic, over the entire wavelength range of the usual approx. > 680nm - > > > approx. 1060nm Ti:Sap lasers common in non linear laser scanning > > > microscopy (and have AR coatings for that entire wavelength range). > They > > > are possibly somewhat more expensive than lambda/2 plates but may > > > nevertheless be the item of choice. I do not have ANY commercial > > > affiliations and, hence, allow myself to mention that I personally have > > > experienced that the achromatic lambda/2 plates from Halle are > excellent > > > units for the purpose, www.b-halle.de . > > > > > > Of course, one also will need a suitable polarization beam splitter and > > > the opto mechanics to position and adjust both, the lambda/2 plate and > > the > > > polarization beam splitter in the appropriate way. Glan prisms, as > Craig > > > mentioned, will be excellent for the purpose but they usually will emit > > > not one but one main and one minor deflected beam under an angle > > different > > > from 90 degrees, while conventional polarization beam splitters will > emit > > > the deflected beam at roughly 90 degrees. Also, check out before buying > > > any beam splitter about potential "un-flatnesses" on the lateral > surfaces > > > of the Glan prism or Polarization beam splitter. A Foster prism might > > also > > > be a good item if you want one beam to continue straight forward and > the > > > other to continue at 45 degrees or so. > > > > > > > > > > > > Also, if you want to install that lambda/2 plate on an optical table > with > > > a lot of other elements like EOMs, photodiodes, spatial filters, beam > > > expanders and the like and if all will be "hidden" behind a metal > curtain > > > to protect laser radiation from becoming dangerous in the lab, then you > > > should consider to install the lambda/2 plate in a motor driven > rotator, > > > which you can control from a PC. > > > > > > Best wishes, > > > > > > Johannes > > > > > > > > > > ***** > > > > To join, leave or search the confocal microscopy listserv, go to: > > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > > ***** > > > > > > > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. > The > > > > prism splits the laser into two beams, with the amount of power going > > > into > > > > each beam governed by the angle of the waveplate. Note you may need > > > > another waveplate to rotate the polarization of one of the beams back > > > into > > > > your microscope. > > > > > > > > Craig > > > > > > > > > > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > > > > <[hidden email]>wrote: > > > > > > > >> ***** > > > >> To join, leave or search the confocal microscopy listserv, go to: > > > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > >> ***** > > > >> > > > >> Dear Listers, > > > >> > > > >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W > at > > > >> 800 > > > >> nm). I know some people did this way to feed one TiSa laser to two > > > >> microscopes. I am wondering if I can get a simple beam splitter for > > > >> this > > > >> purpose. And also, what would be the simple (and reliable) device > you > > > >> would suggest to control the TiSa laser power? Thanks a lot in > > advance > > > >> for > > > >> any suggestion. > > > >> > > > >> Best regards, > > > >> Yuansheng Sun, > > > >> Keck Center for Cellular Imaging > > > >> University of Virginia > > > >> > > > > > > > > > > > > > -- > > > P. Johannes Helm > > > > > > Voice: (+47) 228 51159 (office) > > > Fax: (+47) 228 51499 (office) > > > > > > > > > --------------------------------------------------------------------- > > *SECURITY/CONFIDENTIALITY WARNING: > > This message and any attachments are intended solely for the individual > or > > entity to which they are addressed. This communication may contain > > information that is privileged, confidential, or exempt from disclosure > > under applicable law (e.g., personal health information, research data, > > financial information). Because this e-mail has been sent without > > encryption, individuals other than the intended recipient may be able to > > view the information, forward it to others or tamper with the information > > without the knowledge or consent of the sender. If you are not the > intended > > recipient, or the employee or person responsible for delivering the > message > > to the intended recipient, any dissemination, distribution or copying of > > the communication is strictly prohibited. If you received the > communication > > in error, please notify the sender immediately by replying to this > message > > and deleting the message and any accompanying files from your system. If, > > due to the security risks, you do not wish to receive further > > communications via e-mail, please reply to this message and inform the > > sender that you do not wish to receive further e-mail from the sender. > > (fpc5p) > > --------------------------------------------------------------------- > > > |
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Armstrong, Brian |
Re: A question about splitting a TiSa laser beam and controlling its power
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In reply to this post by yuansheng sun
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, yes you are thinking about it correctly. The "beam dump" will be your second system. We are using AOMs from Carl Zeiss. The AOMs are controlled from the Zeiss operating software so that you can easily adjust power in the software. An alternative method is using a Pockel's Cell to adjust percent power (you would need 2). There are different sources for Pockel's Cells, Thorlabs etc. *They are lambda specific so make sure to match the lambda range you wish to use. Best wishes, Brian D Armstrong PhD Assistant Research Professor Director, Light Microscopy Core Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of yuansheng sun Sent: Tuesday, January 29, 2013 1:57 PM To: [hidden email] Subject: Re: A question about splitting a TiSa laser beam and controlling its power ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you all so much for the great suggestions. Following the suggested direction, I searched and found an application note from Newport (no competing interest). There are two beams out of the Glan-Laser prism - one is the main beam output and the other is called the beam dump. I am wondering if I can achieve any split between the main output and the dump by varying the degree of the half wave plate. I assume the sum of the main output power and the dump power is close to the Ti:Sa laser power (~1.3 W for our system at 800 nm). Brian, can you share your AOM information? Thanks. Sheng On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, yes, and for a rotation mount we used: Newport RSP-IT ( > http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no > competing interest]. > We can adjust the percent power sent to each microscope this way. (The > beam then goes through an AOM which adjusts the percent remaining power to > the microscope, [so we have two AOMs]). > > Cheers, > > Brian D Armstrong PhD > Assistant Research Professor > Director, Light Microscopy Core > Beckman Research Institute > City of Hope > Dept of Neuroscience > 1450 E Duarte Rd > Duarte, CA 91010 > 626-256-4673 x62872 > > > http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Craig Brideau > Sent: Tuesday, January 29, 2013 1:19 PM > To: [hidden email] > Subject: Re: A question about splitting a TiSa laser beam and controlling > its power > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > As Johannes says, you need optomechanics to rotate the waveplate. You can > either use a manual rotation mount (you don't need very fine control, so a > thumb-wheel is fine), or if you are adverse to sticking your hand anywhere > near the beam you can use a motorized mount. In systems with other safety > mechanisms, (i.e. laser blocks and such) I have used manual mounts. In > situations where it will be used by inexperienced users I tend to motorize > things to keep untrained hands away from the laser and optics. > > Craig > > > On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] > >wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Good evening, > > > > Craig has already provided the answer, see below. > > > > I just would like to mention in addition that one can buy lambda/2 > plates, > > which show the lambda/2 effect with minor deviations, only, i.e.: are > > achromatic, over the entire wavelength range of the usual approx. 680nm - > > approx. 1060nm Ti:Sap lasers common in non linear laser scanning > > microscopy (and have AR coatings for that entire wavelength range). They > > are possibly somewhat more expensive than lambda/2 plates but may > > nevertheless be the item of choice. I do not have ANY commercial > > affiliations and, hence, allow myself to mention that I personally have > > experienced that the achromatic lambda/2 plates from Halle are excellent > > units for the purpose, www.b-halle.de . > > > > Of course, one also will need a suitable polarization beam splitter and > > the opto mechanics to position and adjust both, the lambda/2 plate and > the > > polarization beam splitter in the appropriate way. Glan prisms, as Craig > > mentioned, will be excellent for the purpose but they usually will emit > > not one but one main and one minor deflected beam under an angle > different > > from 90 degrees, while conventional polarization beam splitters will emit > > the deflected beam at roughly 90 degrees. Also, check out before buying > > any beam splitter about potential "un-flatnesses" on the lateral surfaces > > of the Glan prism or Polarization beam splitter. A Foster prism might > also > > be a good item if you want one beam to continue straight forward and the > > other to continue at 45 degrees or so. > > > > > > > > Also, if you want to install that lambda/2 plate on an optical table with > > a lot of other elements like EOMs, photodiodes, spatial filters, beam > > expanders and the like and if all will be "hidden" behind a metal curtain > > to protect laser radiation from becoming dangerous in the lab, then you > > should consider to install the lambda/2 plate in a motor driven rotator, > > which you can control from a PC. > > > > Best wishes, > > > > Johannes > > > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. The > > > prism splits the laser into two beams, with the amount of power going > > into > > > each beam governed by the angle of the waveplate. Note you may need > > > another waveplate to rotate the polarization of one of the beams back > > into > > > your microscope. > > > > > > Craig > > > > > > > > > On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > > > <[hidden email]>wrote: > > > > > >> ***** > > >> To join, leave or search the confocal microscopy listserv, go to: > > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > >> ***** > > >> > > >> Dear Listers, > > >> > > >> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W at > > >> 800 > > >> nm). I know some people did this way to feed one TiSa laser to two > > >> microscopes. I am wondering if I can get a simple beam splitter for > > >> this > > >> purpose. And also, what would be the simple (and reliable) device you > > >> would suggest to control the TiSa laser power? Thanks a lot in > advance > > >> for > > >> any suggestion. > > >> > > >> Best regards, > > >> Yuansheng Sun, > > >> Keck Center for Cellular Imaging > > >> University of Virginia > > >> > > > > > > > > > -- > > P. Johannes Helm > > > > Voice: (+47) 228 51159 (office) > > Fax: (+47) 228 51499 (office) > > > > > --------------------------------------------------------------------- > *SECURITY/CONFIDENTIALITY WARNING: > This message and any attachments are intended solely for the individual or > entity to which they are addressed. This communication may contain > information that is privileged, confidential, or exempt from disclosure > under applicable law (e.g., personal health information, research data, > financial information). Because this e-mail has been sent without > encryption, individuals other than the intended recipient may be able to > view the information, forward it to others or tamper with the information > without the knowledge or consent of the sender. If you are not the intended > recipient, or the employee or person responsible for delivering the message > to the intended recipient, any dissemination, distribution or copying of > the communication is strictly prohibited. If you received the communication > in error, please notify the sender immediately by replying to this message > and deleting the message and any accompanying files from your system. If, > due to the security risks, you do not wish to receive further > communications via e-mail, please reply to this message and inform the > sender that you do not wish to receive further e-mail from the sender. > (fpc5p) > --------------------------------------------------------------------- > |
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Mark Cannell-2 |
Re: A question about splitting a TiSa laser beam and controlling its power
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In reply to this post by Craig Brideau
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Have used a polarising beam splitter, but a metal film based neutral density filter works and is much cheaper. Cheers Mark On 29/01/2013, at 10:05 PM, Craig Brideau <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Exactly. The Glan-laser splits the input beam into two output beams. One > output is horizontally polarized, while the other is vertical. The amount > of power in each output depends on the polarization angle of the input > beam. You use a half-waveplate to rotate the input beam polarization to > adjust the power between the two beams. Note that the two outputs will > always add up to the power of the single input; you are splitting the power > of the input beam between the two outputs. You can then use a second > waveplate and Glan-laser to adjust the power of one of the two output arms > by splitting it again. The output of the second cube should be split > between a metal beam dump and your second microscope. > The main advantage of this technique is it allows for smooth analog control > of the power. It is fairly slow since you have to spin a waveplate. If > you need higher speed you can use a pockel's cell instead, but these are > even more expensive than the Glan-laser/waveplate combination. > > Craig > > > On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Thank you all so much for the great suggestions. Following the >> suggested direction, I searched and found an application note from Newport >> (no competing interest). There are two beams out of the Glan-Laser prism - >> one is the main beam output and the other is called the beam dump. I am >> wondering if I can achieve any split between the main output and the dump >> by varying the degree of the half wave plate. I assume the sum of the main >> output power and the dump power is close to the Ti:Sa laser power (~1.3 W >> for our system at 800 nm). >> >> Brian, can you share your AOM information? Thanks. >> >> Sheng >> >> On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email] >>> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Hi, yes, and for a rotation mount we used: Newport RSP-IT ( >>> http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no >>> competing interest]. >>> We can adjust the percent power sent to each microscope this way. (The >>> beam then goes through an AOM which adjusts the percent remaining power >> to >>> the microscope, [so we have two AOMs]). >>> >>> Cheers, >>> >>> Brian D Armstrong PhD >>> Assistant Research Professor >>> Director, Light Microscopy Core >>> Beckman Research Institute >>> City of Hope >>> Dept of Neuroscience >>> 1450 E Duarte Rd >>> Duarte, CA 91010 >>> 626-256-4673 x62872 >>> >>> >>> >> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx >>> >>> >>> -----Original Message----- >>> From: Confocal Microscopy List [mailto:[hidden email]] >>> On Behalf Of Craig Brideau >>> Sent: Tuesday, January 29, 2013 1:19 PM >>> To: [hidden email] >>> Subject: Re: A question about splitting a TiSa laser beam and controlling >>> its power >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> As Johannes says, you need optomechanics to rotate the waveplate. You >> can >>> either use a manual rotation mount (you don't need very fine control, so >> a >>> thumb-wheel is fine), or if you are adverse to sticking your hand >> anywhere >>> near the beam you can use a motorized mount. In systems with other >> safety >>> mechanisms, (i.e. laser blocks and such) I have used manual mounts. In >>> situations where it will be used by inexperienced users I tend to >> motorize >>> things to keep untrained hands away from the laser and optics. >>> >>> Craig >>> >>> >>> On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] >>>> wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> Good evening, >>>> >>>> Craig has already provided the answer, see below. >>>> >>>> I just would like to mention in addition that one can buy lambda/2 >>> plates, >>>> which show the lambda/2 effect with minor deviations, only, i.e.: are >>>> achromatic, over the entire wavelength range of the usual approx. >> 680nm - >>>> approx. 1060nm Ti:Sap lasers common in non linear laser scanning >>>> microscopy (and have AR coatings for that entire wavelength range). >> They >>>> are possibly somewhat more expensive than lambda/2 plates but may >>>> nevertheless be the item of choice. I do not have ANY commercial >>>> affiliations and, hence, allow myself to mention that I personally have >>>> experienced that the achromatic lambda/2 plates from Halle are >> excellent >>>> units for the purpose, www.b-halle.de . >>>> >>>> Of course, one also will need a suitable polarization beam splitter and >>>> the opto mechanics to position and adjust both, the lambda/2 plate and >>> the >>>> polarization beam splitter in the appropriate way. Glan prisms, as >> Craig >>>> mentioned, will be excellent for the purpose but they usually will emit >>>> not one but one main and one minor deflected beam under an angle >>> different >>>> from 90 degrees, while conventional polarization beam splitters will >> emit >>>> the deflected beam at roughly 90 degrees. Also, check out before buying >>>> any beam splitter about potential "un-flatnesses" on the lateral >> surfaces >>>> of the Glan prism or Polarization beam splitter. A Foster prism might >>> also >>>> be a good item if you want one beam to continue straight forward and >> the >>>> other to continue at 45 degrees or so. >>>> >>>> >>>> >>>> Also, if you want to install that lambda/2 plate on an optical table >> with >>>> a lot of other elements like EOMs, photodiodes, spatial filters, beam >>>> expanders and the like and if all will be "hidden" behind a metal >> curtain >>>> to protect laser radiation from becoming dangerous in the lab, then you >>>> should consider to install the lambda/2 plate in a motor driven >> rotator, >>>> which you can control from a PC. >>>> >>>> Best wishes, >>>> >>>> Johannes >>>> >>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. >> The >>>>> prism splits the laser into two beams, with the amount of power going >>>> into >>>>> each beam governed by the angle of the waveplate. Note you may need >>>>> another waveplate to rotate the polarization of one of the beams back >>>> into >>>>> your microscope. >>>>> >>>>> Craig >>>>> >>>>> >>>>> On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun >>>>> <[hidden email]>wrote: >>>>> >>>>>> ***** >>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>> ***** >>>>>> >>>>>> Dear Listers, >>>>>> >>>>>> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W >> at >>>>>> 800 >>>>>> nm). I know some people did this way to feed one TiSa laser to two >>>>>> microscopes. I am wondering if I can get a simple beam splitter for >>>>>> this >>>>>> purpose. And also, what would be the simple (and reliable) device >> you >>>>>> would suggest to control the TiSa laser power? Thanks a lot in >>> advance >>>>>> for >>>>>> any suggestion. >>>>>> >>>>>> Best regards, >>>>>> Yuansheng Sun, >>>>>> Keck Center for Cellular Imaging >>>>>> University of Virginia >>>>>> >>>>> >>>> >>>> >>>> -- >>>> P. Johannes Helm >>>> >>>> Voice: (+47) 228 51159 (office) >>>> Fax: (+47) 228 51499 (office) >>>> >>> >>> >>> --------------------------------------------------------------------- >>> *SECURITY/CONFIDENTIALITY WARNING: >>> This message and any attachments are intended solely for the individual >> or >>> entity to which they are addressed. This communication may contain >>> information that is privileged, confidential, or exempt from disclosure >>> under applicable law (e.g., personal health information, research data, >>> financial information). Because this e-mail has been sent without >>> encryption, individuals other than the intended recipient may be able to >>> view the information, forward it to others or tamper with the information >>> without the knowledge or consent of the sender. If you are not the >> intended >>> recipient, or the employee or person responsible for delivering the >> message >>> to the intended recipient, any dissemination, distribution or copying of >>> the communication is strictly prohibited. If you received the >> communication >>> in error, please notify the sender immediately by replying to this >> message >>> and deleting the message and any accompanying files from your system. If, >>> due to the security risks, you do not wish to receive further >>> communications via e-mail, please reply to this message and inform the >>> sender that you do not wish to receive further e-mail from the sender. >>> (fpc5p) >>> --------------------------------------------------------------------- >>> >> Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology& Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
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Zdenek Svindrych |
Re: A question about splitting a TiSa laser bea m and controlling its power
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Indeed, metal coated ND filter is cheap, but if you use it as a beamsplitter then it may ruin the reflected beam. Filters, as well as conventional beamsplitters, are not quite flat and you still need a diffraction-limitted beam to achieve the 'confocal' effect... Cheers, Zdenek ---------- Původní zpráva ---------- Od: Mark Cannell <[hidden email]> Datum: 30. 1. 2013 Předmět: Re: A question about splitting a TiSa laser beam and controlling its power "***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Have used a polarising beam splitter, but a metal film based neutral density filter works and is much cheaper. Cheers Mark On 29/01/2013, at 10:05 PM, Craig Brideau <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Exactly. The Glan-laser splits the input beam into two output beams. One > output is horizontally polarized, while the other is vertical. The amount > of power in each output depends on the polarization angle of the input > beam. You use a half-waveplate to rotate the input beam polarization to > adjust the power between the two beams. Note that the two outputs will > always add up to the power of the single input; you are splitting the > of the input beam between the two outputs. You can then use a second > waveplate and Glan-laser to adjust the power of one of the two output arms > by splitting it again. The output of the second cube should be split > between a metal beam dump and your second microscope. > The main advantage of this technique is it allows for smooth analog control > of the power. It is fairly slow since you have to spin a waveplate. If > you need higher speed you can use a pockel's cell instead, but these are > even more expensive than the Glan-laser/waveplate combination. > > Craig > > > On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Thank you all so much for the great suggestions. Following the >> suggested direction, I searched and found an application note from Newport >> (no competing interest). There are two beams out of the Glan-Laser prism - >> one is the main beam output and the other is called the beam dump. I am >> wondering if I can achieve any split between the main output and the dump >> by varying the degree of the half wave plate. I assume the sum of the main >> output power and the dump power is close to the Ti:Sa laser power (~1.3 W >> for our system at 800 nm). >> >> Brian, can you share your AOM information? Thanks. >> >> Sheng >> >> On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email] >>> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Hi, yes, and for a rotation mount we used: Newport RSP-IT ( >>> http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no >>> competing interest]. >>> We can adjust the percent power sent to each microscope this way. (The >>> beam then goes through an AOM which adjusts the percent remaining power >> to >>> the microscope, [so we have two AOMs]). >>> >>> Cheers, >>> >>> Brian D Armstrong PhD >>> Assistant Research Professor >>> Director, Light Microscopy Core >>> Beckman Research Institute >>> City of Hope >>> Dept of Neuroscience >>> 1450 E Duarte Rd >>> Duarte, CA 91010 >>> 626-256-4673 x62872 >>> >>> >>> >> http://www.cityofhope.org/research/support/Light-Microscopy-Digital- >>> >>> >>> -----Original Message----- >>> From: Confocal Microscopy List [mailto:[hidden email]] >>> On Behalf Of Craig Brideau >>> Sent: Tuesday, January 29, 2013 1:19 PM >>> To: [hidden email] >>> Subject: Re: A question about splitting a TiSa laser beam and controlling >>> its power >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> As Johannes says, you need optomechanics to rotate the waveplate. You >> can >>> either use a manual rotation mount (you don't need very fine control, so >> a >>> thumb-wheel is fine), or if you are adverse to sticking your hand >> anywhere >>> near the beam you can use a motorized mount. In systems with other >> safety >>> mechanisms, (i.e. laser blocks and such) I have used manual mounts. In >>> situations where it will be used by inexperienced users I tend to >> motorize >>> things to keep untrained hands away from the laser and optics. >>> >>> Craig >>> >>> >>> On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] >>>> wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> Good evening, >>>> >>>> Craig has already provided the answer, see below. >>>> >>>> I just would like to mention in addition that one can buy lambda/2 >>> plates, >>>> which show the lambda/2 effect with minor deviations, only, i.e.: are >>>> achromatic, over the entire wavelength range of the usual approx. >> 680nm - >>>> approx. 1060nm Ti:Sap lasers common in non linear laser scanning >>>> microscopy (and have AR coatings for that entire wavelength range). >> They >>>> are possibly somewhat more expensive than lambda/2 plates but may >>>> nevertheless be the item of choice. I do not have ANY commercial >>>> affiliations and, hence, allow myself to mention that I personally have >>>> experienced that the achromatic lambda/2 plates from Halle are >> excellent >>>> units for the purpose, www.b-halle.de . >>>> >>>> Of course, one also will need a suitable polarization beam splitter and >>>> the opto mechanics to position and adjust both, the lambda/2 plate and >>> the >>>> polarization beam splitter in the appropriate way. Glan prisms, as >> Craig >>>> mentioned, will be excellent for the purpose but they usually will emit >>>> not one but one main and one minor deflected beam under an angle >>> different >>>> from 90 degrees, while conventional polarization beam splitters will >> emit >>>> the deflected beam at roughly 90 degrees. Also, check out before buying >>>> any beam splitter about potential "un-flatnesses" on the lateral >> surfaces >>>> of the Glan prism or Polarization beam splitter. A Foster prism might >>> also >>>> be a good item if you want one beam to continue straight forward and >> the >>>> other to continue at 45 degrees or so. >>>> >>>> >>>> >>>> Also, if you want to install that lambda/2 plate on an optical table >> with >>>> a lot of other elements like EOMs, photodiodes, spatial filters, beam >>>> expanders and the like and if all will be "hidden" behind a metal >> curtain >>>> to protect laser radiation from becoming dangerous in the lab, then you >>>> should consider to install the lambda/2 plate in a motor driven >> rotator, >>>> which you can control from a PC. >>>> >>>> Best wishes, >>>> >>>> Johannes >>>> >>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. >> The >>>>> prism splits the laser into two beams, with the amount of power going >>>> into >>>>> each beam governed by the angle of the waveplate. Note you may need >>>>> another waveplate to rotate the polarization of one of the beams back >>>> into >>>>> your microscope. >>>>> >>>>> Craig >>>>> >>>>> >>>>> On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun >>>>> <[hidden email]>wrote: >>>>> >>>>>> ***** >>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>> ***** >>>>>> >>>>>> Dear Listers, >>>>>> >>>>>> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W >> at >>>>>> 800 >>>>>> nm). I know some people did this way to feed one TiSa laser to two >>>>>> microscopes. I am wondering if I can get a simple beam splitter for >>>>>> this >>>>>> purpose. And also, what would be the simple (and reliable) device >> you >>>>>> would suggest to control the TiSa laser power? Thanks a lot in >>> advance >>>>>> for >>>>>> any suggestion. >>>>>> >>>>>> Best regards, >>>>>> Yuansheng Sun, >>>>>> Keck Center for Cellular Imaging >>>>>> University of Virginia >>>>>> >>>>> >>>> >>>> >>>> -- >>>> P. Johannes Helm >>>> >>>> Voice: (+47) 228 51159 (office) >>>> Fax: (+47) 228 51499 (office) >>>> >>> >>> >>> --------------------------------------------------------------------- >>> *SECURITY/CONFIDENTIALITY WARNING: >>> This message and any attachments are intended solely for the individual >> or >>> entity to which they are addressed. This communication may contain >>> information that is privileged, confidential, or exempt from disclosure >>> under applicable law (e.g., personal health information, research data, >>> financial information). Because this e-mail has been sent without >>> encryption, individuals other than the intended recipient may be able to >>> view the information, forward it to others or tamper with the >>> without the knowledge or consent of the sender. If you are not the >> intended >>> recipient, or the employee or person responsible for delivering the >> message >>> to the intended recipient, any dissemination, distribution or copying of >>> the communication is strictly prohibited. If you received the >> communication >>> in error, please notify the sender immediately by replying to this >> message >>> and deleting the message and any accompanying files from your system. >>> due to the security risks, you do not wish to receive further >>> communications via e-mail, please reply to this message and inform the >>> sender that you do not wish to receive further e-mail from the sender. >>> (fpc5p) >>> --------------------------------------------------------------------- >>> >> Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology& Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email]" |
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Mark Cannell-2 |
Re: A question about splitting a TiSa laser bea m and controlling its power
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** That may be so for some filters but it has not proven to be the case in my experience -the flatness over the bean diameter was clearly sufficient. We've also used a rotating variable neutral density filter as beam splitter between two microscopes. Cheers On 30/01/2013, at 9:28 AM, Zdenek Svindrych <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Indeed, metal coated ND filter is cheap, but if you use it as a beamsplitter > then it may ruin the reflected beam. Filters, as well as conventional > beamsplitters, are not quite flat and you still need a diffraction-limitted > beam to achieve the 'confocal' effect... > > Cheers, Zdenek > > > > ---------- Původní zpráva ---------- > Od: Mark Cannell <[hidden email]> > Datum: 30. 1. 2013 > Předmět: Re: A question about splitting a TiSa laser beam and controlling > its power > > "***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Have used a polarising beam splitter, but a metal film based neutral density > filter works and is much cheaper. > > Cheers Mark > > > On 29/01/2013, at 10:05 PM, Craig Brideau <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Exactly. The Glan-laser splits the input beam into two output beams. One >> output is horizontally polarized, while the other is vertical. The amount >> of power in each output depends on the polarization angle of the input >> beam. You use a half-waveplate to rotate the input beam polarization to >> adjust the power between the two beams. Note that the two outputs will >> always add up to the power of the single input; you are splitting the > power >> of the input beam between the two outputs. You can then use a second >> waveplate and Glan-laser to adjust the power of one of the two output arms >> by splitting it again. The output of the second cube should be split >> between a metal beam dump and your second microscope. >> The main advantage of this technique is it allows for smooth analog > control >> of the power. It is fairly slow since you have to spin a waveplate. If >> you need higher speed you can use a pockel's cell instead, but these are >> even more expensive than the Glan-laser/waveplate combination. >> >> Craig >> >> >> On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun <[hidden email]> > wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Thank you all so much for the great suggestions. Following the >>> suggested direction, I searched and found an application note from > Newport >>> (no competing interest). There are two beams out of the Glan-Laser prism > - >>> one is the main beam output and the other is called the beam dump. I am >>> wondering if I can achieve any split between the main output and the dump >>> by varying the degree of the half wave plate. I assume the sum of the > main >>> output power and the dump power is close to the Ti:Sa laser power (~1.3 W >>> for our system at 800 nm). >>> >>> Brian, can you share your AOM information? Thanks. >>> >>> Sheng >>> >>> On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email] >>>> wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> Hi, yes, and for a rotation mount we used: Newport RSP-IT ( >>>> http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) [no >>>> competing interest]. >>>> We can adjust the percent power sent to each microscope this way. (The >>>> beam then goes through an AOM which adjusts the percent remaining power >>> to >>>> the microscope, [so we have two AOMs]). >>>> >>>> Cheers, >>>> >>>> Brian D Armstrong PhD >>>> Assistant Research Professor >>>> Director, Light Microscopy Core >>>> Beckman Research Institute >>>> City of Hope >>>> Dept of Neuroscience >>>> 1450 E Duarte Rd >>>> Duarte, CA 91010 >>>> 626-256-4673 x62872 >>>> >>>> >>>> >>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital- > Imaging/Pages/default.aspx >>>> >>>> >>>> -----Original Message----- >>>> From: Confocal Microscopy List [mailto:[hidden email]] >>>> On Behalf Of Craig Brideau >>>> Sent: Tuesday, January 29, 2013 1:19 PM >>>> To: [hidden email] >>>> Subject: Re: A question about splitting a TiSa laser beam and > controlling >>>> its power >>>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> As Johannes says, you need optomechanics to rotate the waveplate. You >>> can >>>> either use a manual rotation mount (you don't need very fine control, so >>> a >>>> thumb-wheel is fine), or if you are adverse to sticking your hand >>> anywhere >>>> near the beam you can use a motorized mount. In systems with other >>> safety >>>> mechanisms, (i.e. laser blocks and such) I have used manual mounts. In >>>> situations where it will be used by inexperienced users I tend to >>> motorize >>>> things to keep untrained hands away from the laser and optics. >>>> >>>> Craig >>>> >>>> >>>> On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm <[hidden email] >>>>> wrote: >>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> Good evening, >>>>> >>>>> Craig has already provided the answer, see below. >>>>> >>>>> I just would like to mention in addition that one can buy lambda/2 >>>> plates, >>>>> which show the lambda/2 effect with minor deviations, only, i.e.: are >>>>> achromatic, over the entire wavelength range of the usual approx. >>> 680nm - >>>>> approx. 1060nm Ti:Sap lasers common in non linear laser scanning >>>>> microscopy (and have AR coatings for that entire wavelength range). >>> They >>>>> are possibly somewhat more expensive than lambda/2 plates but may >>>>> nevertheless be the item of choice. I do not have ANY commercial >>>>> affiliations and, hence, allow myself to mention that I personally have >>>>> experienced that the achromatic lambda/2 plates from Halle are >>> excellent >>>>> units for the purpose, www.b-halle.de . >>>>> >>>>> Of course, one also will need a suitable polarization beam splitter and >>>>> the opto mechanics to position and adjust both, the lambda/2 plate and >>>> the >>>>> polarization beam splitter in the appropriate way. Glan prisms, as >>> Craig >>>>> mentioned, will be excellent for the purpose but they usually will emit >>>>> not one but one main and one minor deflected beam under an angle >>>> different >>>>> from 90 degrees, while conventional polarization beam splitters will >>> emit >>>>> the deflected beam at roughly 90 degrees. Also, check out before buying >>>>> any beam splitter about potential "un-flatnesses" on the lateral >>> surfaces >>>>> of the Glan prism or Polarization beam splitter. A Foster prism might >>>> also >>>>> be a good item if you want one beam to continue straight forward and >>> the >>>>> other to continue at 45 degrees or so. >>>>> >>>>> >>>>> >>>>> Also, if you want to install that lambda/2 plate on an optical table >>> with >>>>> a lot of other elements like EOMs, photodiodes, spatial filters, beam >>>>> expanders and the like and if all will be "hidden" behind a metal >>> curtain >>>>> to protect laser radiation from becoming dangerous in the lab, then you >>>>> should consider to install the lambda/2 plate in a motor driven >>> rotator, >>>>> which you can control from a PC. >>>>> >>>>> Best wishes, >>>>> >>>>> Johannes >>>>> >>>>> >>>>>> ***** >>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>> ***** >>>>>> >>>>>> A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. >>> The >>>>>> prism splits the laser into two beams, with the amount of power going >>>>> into >>>>>> each beam governed by the angle of the waveplate. Note you may need >>>>>> another waveplate to rotate the polarization of one of the beams back >>>>> into >>>>>> your microscope. >>>>>> >>>>>> Craig >>>>>> >>>>>> >>>>>> On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun >>>>>> <[hidden email]>wrote: >>>>>> >>>>>>> ***** >>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>> ***** >>>>>>> >>>>>>> Dear Listers, >>>>>>> >>>>>>> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W >>> at >>>>>>> 800 >>>>>>> nm). I know some people did this way to feed one TiSa laser to two >>>>>>> microscopes. I am wondering if I can get a simple beam splitter for >>>>>>> this >>>>>>> purpose. And also, what would be the simple (and reliable) device >>> you >>>>>>> would suggest to control the TiSa laser power? Thanks a lot in >>>> advance >>>>>>> for >>>>>>> any suggestion. >>>>>>> >>>>>>> Best regards, >>>>>>> Yuansheng Sun, >>>>>>> Keck Center for Cellular Imaging >>>>>>> University of Virginia >>>>>>> >>>>>> >>>>> >>>>> >>>>> -- >>>>> P. Johannes Helm >>>>> >>>>> Voice: (+47) 228 51159 (office) >>>>> Fax: (+47) 228 51499 (office) >>>>> >>>> >>>> >>>> --------------------------------------------------------------------- >>>> *SECURITY/CONFIDENTIALITY WARNING: >>>> This message and any attachments are intended solely for the individual >>> or >>>> entity to which they are addressed. This communication may contain >>>> information that is privileged, confidential, or exempt from disclosure >>>> under applicable law (e.g., personal health information, research data, >>>> financial information). Because this e-mail has been sent without >>>> encryption, individuals other than the intended recipient may be able to >>>> view the information, forward it to others or tamper with the > information >>>> without the knowledge or consent of the sender. If you are not the >>> intended >>>> recipient, or the employee or person responsible for delivering the >>> message >>>> to the intended recipient, any dissemination, distribution or copying of >>>> the communication is strictly prohibited. If you received the >>> communication >>>> in error, please notify the sender immediately by replying to this >>> message >>>> and deleting the message and any accompanying files from your system. > If, >>>> due to the security risks, you do not wish to receive further >>>> communications via e-mail, please reply to this message and inform the >>>> sender that you do not wish to receive further e-mail from the sender. >>>> (fpc5p) >>>> --------------------------------------------------------------------- >>>> >>> > > Mark B. Cannell Ph.D. FRSNZ > Professor of Cardiac Cell Biology > School of Physiology& Pharmacology > Medical Sciences Building > University of Bristol > Bristol > BS8 1TD UK > > [hidden email]" Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology& Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
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Craig Brideau |
Re: A question about splitting a TiSa laser bea m and controlling its power
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** There are a couple advantages of the Glan-Laser compared to an ND filter that may matter for some users. First, the GL gives you a very well defined polarization state out of each surface. Depending on the angle of an ND filter relative to the beam passing through it, you can have some spatio-spectral or -temporal effects. This may or may not matter, depending on how picky what you are doing is. Second is damage threshold. A GL is made of highest-grade calcite, so you can throw everything a Ti:Saph can generate at one and never come even close to damaging it. Reflective ND filters are also robust, but if the reflective coating becomes compromised the laser will chew right through it over time. Fingerprints and dust can do this. The GL does all of its work on the *inside* surface which can't get contaminated. My 2 cents Craig On Wed, Jan 30, 2013 at 2:49 AM, Mark Cannell <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > That may be so for some filters but it has not proven to be the case in my > experience -the flatness over the bean diameter was clearly sufficient. > We've also used a rotating variable neutral density filter as beam splitter > between two microscopes. > > Cheers > > > On 30/01/2013, at 9:28 AM, Zdenek Svindrych <[hidden email]> wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Indeed, metal coated ND filter is cheap, but if you use it as a > beamsplitter > > then it may ruin the reflected beam. Filters, as well as conventional > > beamsplitters, are not quite flat and you still need a > diffraction-limitted > > beam to achieve the 'confocal' effect... > > > > Cheers, Zdenek > > > > > > > > ---------- Původní zpráva ---------- > > Od: Mark Cannell <[hidden email]> > > Datum: 30. 1. 2013 > > Předmět: Re: A question about splitting a TiSa laser beam and controlling > > its power > > > > "***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Have used a polarising beam splitter, but a metal film based neutral > density > > filter works and is much cheaper. > > > > Cheers Mark > > > > > > On 29/01/2013, at 10:05 PM, Craig Brideau <[hidden email]> > wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> Exactly. The Glan-laser splits the input beam into two output beams. One > >> output is horizontally polarized, while the other is vertical. The > amount > >> of power in each output depends on the polarization angle of the input > >> beam. You use a half-waveplate to rotate the input beam polarization to > >> adjust the power between the two beams. Note that the two outputs will > >> always add up to the power of the single input; you are splitting the > > power > >> of the input beam between the two outputs. You can then use a second > >> waveplate and Glan-laser to adjust the power of one of the two output > arms > >> by splitting it again. The output of the second cube should be split > >> between a metal beam dump and your second microscope. > >> The main advantage of this technique is it allows for smooth analog > > control > >> of the power. It is fairly slow since you have to spin a waveplate. If > >> you need higher speed you can use a pockel's cell instead, but these are > >> even more expensive than the Glan-laser/waveplate combination. > >> > >> Craig > >> > >> > >> On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun <[hidden email] > > > > wrote: > >> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> ***** > >>> > >>> Thank you all so much for the great suggestions. Following the > >>> suggested direction, I searched and found an application note from > > Newport > >>> (no competing interest). There are two beams out of the Glan-Laser > prism > > - > >>> one is the main beam output and the other is called the beam dump. I am > >>> wondering if I can achieve any split between the main output and the > dump > >>> by varying the degree of the half wave plate. I assume the sum of the > > main > >>> output power and the dump power is close to the Ti:Sa laser power > (~1.3 W > >>> for our system at 800 nm). > >>> > >>> Brian, can you share your AOM information? Thanks. > >>> > >>> Sheng > >>> > >>> On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian <[hidden email] > >>>> wrote: > >>> > >>>> ***** > >>>> To join, leave or search the confocal microscopy listserv, go to: > >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>> ***** > >>>> > >>>> Hi, yes, and for a rotation mount we used: Newport RSP-IT ( > >>>> http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) > [no > >>>> competing interest]. > >>>> We can adjust the percent power sent to each microscope this way. (The > >>>> beam then goes through an AOM which adjusts the percent remaining > power > >>> to > >>>> the microscope, [so we have two AOMs]). > >>>> > >>>> Cheers, > >>>> > >>>> Brian D Armstrong PhD > >>>> Assistant Research Professor > >>>> Director, Light Microscopy Core > >>>> Beckman Research Institute > >>>> City of Hope > >>>> Dept of Neuroscience > >>>> 1450 E Duarte Rd > >>>> Duarte, CA 91010 > >>>> 626-256-4673 x62872 > >>>> > >>>> > >>>> > >>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital- > > Imaging/Pages/default.aspx > >>>> > >>>> > >>>> -----Original Message----- > >>>> From: Confocal Microscopy List [mailto: > [hidden email]] > >>>> On Behalf Of Craig Brideau > >>>> Sent: Tuesday, January 29, 2013 1:19 PM > >>>> To: [hidden email] > >>>> Subject: Re: A question about splitting a TiSa laser beam and > > controlling > >>>> its power > >>>> > >>>> ***** > >>>> To join, leave or search the confocal microscopy listserv, go to: > >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>> ***** > >>>> > >>>> As Johannes says, you need optomechanics to rotate the waveplate. You > >>> can > >>>> either use a manual rotation mount (you don't need very fine control, > so > >>> a > >>>> thumb-wheel is fine), or if you are adverse to sticking your hand > >>> anywhere > >>>> near the beam you can use a motorized mount. In systems with other > >>> safety > >>>> mechanisms, (i.e. laser blocks and such) I have used manual mounts. In > >>>> situations where it will be used by inexperienced users I tend to > >>> motorize > >>>> things to keep untrained hands away from the laser and optics. > >>>> > >>>> Craig > >>>> > >>>> > >>>> On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm < > [hidden email] > >>>>> wrote: > >>>> > >>>>> ***** > >>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>> ***** > >>>>> > >>>>> Good evening, > >>>>> > >>>>> Craig has already provided the answer, see below. > >>>>> > >>>>> I just would like to mention in addition that one can buy lambda/2 > >>>> plates, > >>>>> which show the lambda/2 effect with minor deviations, only, i.e.: are > >>>>> achromatic, over the entire wavelength range of the usual approx. > >>> 680nm - > >>>>> approx. 1060nm Ti:Sap lasers common in non linear laser scanning > >>>>> microscopy (and have AR coatings for that entire wavelength range). > >>> They > >>>>> are possibly somewhat more expensive than lambda/2 plates but may > >>>>> nevertheless be the item of choice. I do not have ANY commercial > >>>>> affiliations and, hence, allow myself to mention that I personally > have > >>>>> experienced that the achromatic lambda/2 plates from Halle are > >>> excellent > >>>>> units for the purpose, www.b-halle.de . > >>>>> > >>>>> Of course, one also will need a suitable polarization beam splitter > and > >>>>> the opto mechanics to position and adjust both, the lambda/2 plate > and > >>>> the > >>>>> polarization beam splitter in the appropriate way. Glan prisms, as > >>> Craig > >>>>> mentioned, will be excellent for the purpose but they usually will > emit > >>>>> not one but one main and one minor deflected beam under an angle > >>>> different > >>>>> from 90 degrees, while conventional polarization beam splitters will > >>> emit > >>>>> the deflected beam at roughly 90 degrees. Also, check out before > buying > >>>>> any beam splitter about potential "un-flatnesses" on the lateral > >>> surfaces > >>>>> of the Glan prism or Polarization beam splitter. A Foster prism might > >>>> also > >>>>> be a good item if you want one beam to continue straight forward and > >>> the > >>>>> other to continue at 45 degrees or so. > >>>>> > >>>>> > >>>>> > >>>>> Also, if you want to install that lambda/2 plate on an optical table > >>> with > >>>>> a lot of other elements like EOMs, photodiodes, spatial filters, beam > >>>>> expanders and the like and if all will be "hidden" behind a metal > >>> curtain > >>>>> to protect laser radiation from becoming dangerous in the lab, then > you > >>>>> should consider to install the lambda/2 plate in a motor driven > >>> rotator, > >>>>> which you can control from a PC. > >>>>> > >>>>> Best wishes, > >>>>> > >>>>> Johannes > >>>>> > >>>>> > >>>>>> ***** > >>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>> ***** > >>>>>> > >>>>>> A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. > >>> The > >>>>>> prism splits the laser into two beams, with the amount of power > going > >>>>> into > >>>>>> each beam governed by the angle of the waveplate. Note you may need > >>>>>> another waveplate to rotate the polarization of one of the beams > back > >>>>> into > >>>>>> your microscope. > >>>>>> > >>>>>> Craig > >>>>>> > >>>>>> > >>>>>> On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun > >>>>>> <[hidden email]>wrote: > >>>>>> > >>>>>>> ***** > >>>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>>> ***** > >>>>>>> > >>>>>>> Dear Listers, > >>>>>>> > >>>>>>> I want to split the laser beam from a TiSa laser (700~1000 nm, 1.3W > >>> at > >>>>>>> 800 > >>>>>>> nm). I know some people did this way to feed one TiSa laser to two > >>>>>>> microscopes. I am wondering if I can get a simple beam splitter for > >>>>>>> this > >>>>>>> purpose. And also, what would be the simple (and reliable) device > >>> you > >>>>>>> would suggest to control the TiSa laser power? Thanks a lot in > >>>> advance > >>>>>>> for > >>>>>>> any suggestion. > >>>>>>> > >>>>>>> Best regards, > >>>>>>> Yuansheng Sun, > >>>>>>> Keck Center for Cellular Imaging > >>>>>>> University of Virginia > >>>>>>> > >>>>>> > >>>>> > >>>>> > >>>>> -- > >>>>> P. Johannes Helm > >>>>> > >>>>> Voice: (+47) 228 51159 (office) > >>>>> Fax: (+47) 228 51499 (office) > >>>>> > >>>> > >>>> > >>>> --------------------------------------------------------------------- > >>>> *SECURITY/CONFIDENTIALITY WARNING: > >>>> This message and any attachments are intended solely for the > individual > >>> or > >>>> entity to which they are addressed. This communication may contain > >>>> information that is privileged, confidential, or exempt from > disclosure > >>>> under applicable law (e.g., personal health information, research > data, > >>>> financial information). Because this e-mail has been sent without > >>>> encryption, individuals other than the intended recipient may be able > to > >>>> view the information, forward it to others or tamper with the > > information > >>>> without the knowledge or consent of the sender. If you are not the > >>> intended > >>>> recipient, or the employee or person responsible for delivering the > >>> message > >>>> to the intended recipient, any dissemination, distribution or copying > of > >>>> the communication is strictly prohibited. If you received the > >>> communication > >>>> in error, please notify the sender immediately by replying to this > >>> message > >>>> and deleting the message and any accompanying files from your system. > > If, > >>>> due to the security risks, you do not wish to receive further > >>>> communications via e-mail, please reply to this message and inform the > >>>> sender that you do not wish to receive further e-mail from the sender. > >>>> (fpc5p) > >>>> --------------------------------------------------------------------- > >>>> > >>> > > > > Mark B. Cannell Ph.D. FRSNZ > > Professor of Cardiac Cell Biology > > School of Physiology& Pharmacology > > Medical Sciences Building > > University of Bristol > > Bristol > > BS8 1TD UK > > > > [hidden email]" > > Mark B. Cannell Ph.D. FRSNZ > Professor of Cardiac Cell Biology > School of Physiology& Pharmacology > Medical Sciences Building > University of Bristol > Bristol > BS8 1TD UK > > [hidden email] > |
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Johannes Helm |
Re: A question about splitting a TiSa laser bea m and controlling its power
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just a short comment, since Craig writes about an important issue below: Some companies offer Glan prisms for high laser powers. Somewhat more expensive than the usual Glan prisms, the "High Power" versions feature specially polished surfaces to minimize any straylight and are rated for excessively high power densities. They are possibly more suitable for Ti:Sap lasers with sub-picoseconds pulselengths. Best wishes, Johannes > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > There are a couple advantages of the Glan-Laser compared to an ND filter > that may matter for some users. First, the GL gives you a very well > defined polarization state out of each surface. Depending on the angle of > an ND filter relative to the beam passing through it, you can have some > spatio-spectral or -temporal effects. This may or may not matter, > depending on how picky what you are doing is. > Second is damage threshold. A GL is made of highest-grade calcite, so you > can throw everything a Ti:Saph can generate at one and never come even > close to damaging it. Reflective ND filters are also robust, but if the > reflective coating becomes compromised the laser will chew right through > it > over time. Fingerprints and dust can do this. The GL does all of its > work > on the *inside* surface which can't get contaminated. > > My 2 cents > > Craig > > > On Wed, Jan 30, 2013 at 2:49 AM, Mark Cannell > <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> That may be so for some filters but it has not proven to be the case in >> my >> experience -the flatness over the bean diameter was clearly sufficient. >> We've also used a rotating variable neutral density filter as beam >> splitter >> between two microscopes. >> >> Cheers >> >> >> On 30/01/2013, at 9:28 AM, Zdenek Svindrych <[hidden email]> wrote: >> >> > ***** >> > To join, leave or search the confocal microscopy listserv, go to: >> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > ***** >> > >> > Indeed, metal coated ND filter is cheap, but if you use it as a >> beamsplitter >> > then it may ruin the reflected beam. Filters, as well as conventional >> > beamsplitters, are not quite flat and you still need a >> diffraction-limitted >> > beam to achieve the 'confocal' effect... >> > >> > Cheers, Zdenek >> > >> > >> > >> > ---------- P?vodní zpráva ---------- >> > Od: Mark Cannell <[hidden email]> >> > Datum: 30. 1. 2013 >> > P?edm?t: Re: A question about splitting a TiSa laser beam and >> controlling >> > its power >> > >> > "***** >> > To join, leave or search the confocal microscopy listserv, go to: >> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > ***** >> > >> > Have used a polarising beam splitter, but a metal film based neutral >> density >> > filter works and is much cheaper. >> > >> > Cheers Mark >> > >> > >> > On 29/01/2013, at 10:05 PM, Craig Brideau <[hidden email]> >> wrote: >> > >> >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >> ***** >> >> >> >> Exactly. The Glan-laser splits the input beam into two output beams. >> One >> >> output is horizontally polarized, while the other is vertical. The >> amount >> >> of power in each output depends on the polarization angle of the >> input >> >> beam. You use a half-waveplate to rotate the input beam polarization >> to >> >> adjust the power between the two beams. Note that the two outputs >> will >> >> always add up to the power of the single input; you are splitting the >> > power >> >> of the input beam between the two outputs. You can then use a second >> >> waveplate and Glan-laser to adjust the power of one of the two output >> arms >> >> by splitting it again. The output of the second cube should be split >> >> between a metal beam dump and your second microscope. >> >> The main advantage of this technique is it allows for smooth analog >> > control >> >> of the power. It is fairly slow since you have to spin a waveplate. >> If >> >> you need higher speed you can use a pockel's cell instead, but these >> are >> >> even more expensive than the Glan-laser/waveplate combination. >> >> >> >> Craig >> >> >> >> >> >> On Tue, Jan 29, 2013 at 2:56 PM, yuansheng sun >> <[hidden email] >> > >> > wrote: >> >> >> >>> ***** >> >>> To join, leave or search the confocal microscopy listserv, go to: >> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>> ***** >> >>> >> >>> Thank you all so much for the great suggestions. Following the >> >>> suggested direction, I searched and found an application note from >> > Newport >> >>> (no competing interest). There are two beams out of the Glan-Laser >> prism >> > - >> >>> one is the main beam output and the other is called the beam dump. I >> am >> >>> wondering if I can achieve any split between the main output and the >> dump >> >>> by varying the degree of the half wave plate. I assume the sum of >> the >> > main >> >>> output power and the dump power is close to the Ti:Sa laser power >> (~1.3 W >> >>> for our system at 800 nm). >> >>> >> >>> Brian, can you share your AOM information? Thanks. >> >>> >> >>> Sheng >> >>> >> >>> On Tue, Jan 29, 2013 at 4:36 PM, Armstrong, Brian >> <[hidden email] >> >>>> wrote: >> >>> >> >>>> ***** >> >>>> To join, leave or search the confocal microscopy listserv, go to: >> >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>>> ***** >> >>>> >> >>>> Hi, yes, and for a rotation mount we used: Newport RSP-IT ( >> >>>> http://www.newport.com/Optic-Rotation-Mounts/177122/1033/info.aspx) >> [no >> >>>> competing interest]. >> >>>> We can adjust the percent power sent to each microscope this way. >> (The >> >>>> beam then goes through an AOM which adjusts the percent remaining >> power >> >>> to >> >>>> the microscope, [so we have two AOMs]). >> >>>> >> >>>> Cheers, >> >>>> >> >>>> Brian D Armstrong PhD >> >>>> Assistant Research Professor >> >>>> Director, Light Microscopy Core >> >>>> Beckman Research Institute >> >>>> City of Hope >> >>>> Dept of Neuroscience >> >>>> 1450 E Duarte Rd >> >>>> Duarte, CA 91010 >> >>>> 626-256-4673 x62872 >> >>>> >> >>>> >> >>>> >> >>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital- >> > Imaging/Pages/default.aspx >> >>>> >> >>>> >> >>>> -----Original Message----- >> >>>> From: Confocal Microscopy List [mailto: >> [hidden email]] >> >>>> On Behalf Of Craig Brideau >> >>>> Sent: Tuesday, January 29, 2013 1:19 PM >> >>>> To: [hidden email] >> >>>> Subject: Re: A question about splitting a TiSa laser beam and >> > controlling >> >>>> its power >> >>>> >> >>>> ***** >> >>>> To join, leave or search the confocal microscopy listserv, go to: >> >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>>> ***** >> >>>> >> >>>> As Johannes says, you need optomechanics to rotate the waveplate. >> You >> >>> can >> >>>> either use a manual rotation mount (you don't need very fine >> control, >> so >> >>> a >> >>>> thumb-wheel is fine), or if you are adverse to sticking your hand >> >>> anywhere >> >>>> near the beam you can use a motorized mount. In systems with other >> >>> safety >> >>>> mechanisms, (i.e. laser blocks and such) I have used manual mounts. >> In >> >>>> situations where it will be used by inexperienced users I tend to >> >>> motorize >> >>>> things to keep untrained hands away from the laser and optics. >> >>>> >> >>>> Craig >> >>>> >> >>>> >> >>>> On Tue, Jan 29, 2013 at 2:05 PM, Johannes Helm < >> [hidden email] >> >>>>> wrote: >> >>>> >> >>>>> ***** >> >>>>> To join, leave or search the confocal microscopy listserv, go to: >> >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>>>> ***** >> >>>>> >> >>>>> Good evening, >> >>>>> >> >>>>> Craig has already provided the answer, see below. >> >>>>> >> >>>>> I just would like to mention in addition that one can buy lambda/2 >> >>>> plates, >> >>>>> which show the lambda/2 effect with minor deviations, only, i.e.: >> are >> >>>>> achromatic, over the entire wavelength range of the usual approx. >> >>> 680nm - >> >>>>> approx. 1060nm Ti:Sap lasers common in non linear laser scanning >> >>>>> microscopy (and have AR coatings for that entire wavelength >> range). >> >>> They >> >>>>> are possibly somewhat more expensive than lambda/2 plates but may >> >>>>> nevertheless be the item of choice. I do not have ANY commercial >> >>>>> affiliations and, hence, allow myself to mention that I personally >> have >> >>>>> experienced that the achromatic lambda/2 plates from Halle are >> >>> excellent >> >>>>> units for the purpose, www.b-halle.de . >> >>>>> >> >>>>> Of course, one also will need a suitable polarization beam >> splitter >> and >> >>>>> the opto mechanics to position and adjust both, the lambda/2 plate >> and >> >>>> the >> >>>>> polarization beam splitter in the appropriate way. Glan prisms, as >> >>> Craig >> >>>>> mentioned, will be excellent for the purpose but they usually will >> emit >> >>>>> not one but one main and one minor deflected beam under an angle >> >>>> different >> >>>>> from 90 degrees, while conventional polarization beam splitters >> will >> >>> emit >> >>>>> the deflected beam at roughly 90 degrees. Also, check out before >> buying >> >>>>> any beam splitter about potential "un-flatnesses" on the lateral >> >>> surfaces >> >>>>> of the Glan prism or Polarization beam splitter. A Foster prism >> might >> >>>> also >> >>>>> be a good item if you want one beam to continue straight forward >> and >> >>> the >> >>>>> other to continue at 45 degrees or so. >> >>>>> >> >>>>> >> >>>>> >> >>>>> Also, if you want to install that lambda/2 plate on an optical >> table >> >>> with >> >>>>> a lot of other elements like EOMs, photodiodes, spatial filters, >> beam >> >>>>> expanders and the like and if all will be "hidden" behind a metal >> >>> curtain >> >>>>> to protect laser radiation from becoming dangerous in the lab, >> then >> you >> >>>>> should consider to install the lambda/2 plate in a motor driven >> >>> rotator, >> >>>>> which you can control from a PC. >> >>>>> >> >>>>> Best wishes, >> >>>>> >> >>>>> Johannes >> >>>>> >> >>>>> >> >>>>>> ***** >> >>>>>> To join, leave or search the confocal microscopy listserv, go to: >> >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>>>>> ***** >> >>>>>> >> >>>>>> A Glan-Laser prism and half-waveplate (NIR Achromatic) will work. >> >>> The >> >>>>>> prism splits the laser into two beams, with the amount of power >> going >> >>>>> into >> >>>>>> each beam governed by the angle of the waveplate. Note you may >> need >> >>>>>> another waveplate to rotate the polarization of one of the beams >> back >> >>>>> into >> >>>>>> your microscope. >> >>>>>> >> >>>>>> Craig >> >>>>>> >> >>>>>> >> >>>>>> On Tue, Jan 29, 2013 at 11:41 AM, yuansheng sun >> >>>>>> <[hidden email]>wrote: >> >>>>>> >> >>>>>>> ***** >> >>>>>>> To join, leave or search the confocal microscopy listserv, go >> to: >> >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>>>>>> ***** >> >>>>>>> >> >>>>>>> Dear Listers, >> >>>>>>> >> >>>>>>> I want to split the laser beam from a TiSa laser (700~1000 nm, >> 1.3W >> >>> at >> >>>>>>> 800 >> >>>>>>> nm). I know some people did this way to feed one TiSa laser to >> two >> >>>>>>> microscopes. I am wondering if I can get a simple beam splitter >> for >> >>>>>>> this >> >>>>>>> purpose. And also, what would be the simple (and reliable) >> device >> >>> you >> >>>>>>> would suggest to control the TiSa laser power? Thanks a lot in >> >>>> advance >> >>>>>>> for >> >>>>>>> any suggestion. >> >>>>>>> >> >>>>>>> Best regards, >> >>>>>>> Yuansheng Sun, >> >>>>>>> Keck Center for Cellular Imaging >> >>>>>>> University of Virginia >> >>>>>>> >> >>>>>> >> >>>>> >> >>>>> >> >>>>> -- >> >>>>> P. Johannes Helm >> >>>>> >> >>>>> Voice: (+47) 228 51159 (office) >> >>>>> Fax: (+47) 228 51499 (office) >> >>>>> >> >>>> >> >>>> >> >>>> --------------------------------------------------------------------- >> >>>> *SECURITY/CONFIDENTIALITY WARNING: >> >>>> This message and any attachments are intended solely for the >> individual >> >>> or >> >>>> entity to which they are addressed. This communication may contain >> >>>> information that is privileged, confidential, or exempt from >> disclosure >> >>>> under applicable law (e.g., personal health information, research >> data, >> >>>> financial information). Because this e-mail has been sent without >> >>>> encryption, individuals other than the intended recipient may be >> able >> to >> >>>> view the information, forward it to others or tamper with the >> > information >> >>>> without the knowledge or consent of the sender. If you are not the >> >>> intended >> >>>> recipient, or the employee or person responsible for delivering the >> >>> message >> >>>> to the intended recipient, any dissemination, distribution or >> copying >> of >> >>>> the communication is strictly prohibited. If you received the >> >>> communication >> >>>> in error, please notify the sender immediately by replying to this >> >>> message >> >>>> and deleting the message and any accompanying files from your >> system. >> > If, >> >>>> due to the security risks, you do not wish to receive further >> >>>> communications via e-mail, please reply to this message and inform >> the >> >>>> sender that you do not wish to receive further e-mail from the >> sender. >> >>>> (fpc5p) >> >>>> --------------------------------------------------------------------- >> >>>> >> >>> >> > >> > Mark B. Cannell Ph.D. FRSNZ >> > Professor of Cardiac Cell Biology >> > School of Physiology& Pharmacology >> > Medical Sciences Building >> > University of Bristol >> > Bristol >> > BS8 1TD UK >> > >> > [hidden email]" >> >> Mark B. Cannell Ph.D. FRSNZ >> Professor of Cardiac Cell Biology >> School of Physiology& Pharmacology >> Medical Sciences Building >> University of Bristol >> Bristol >> BS8 1TD UK >> >> [hidden email] >> > -- P. Johannes Helm Voice: (+47) 228 51159 (office) Fax: (+47) 228 51499 (office) |
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