A1
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi colleagues Did anybodyy have hands on the nikon A1? regards Heiko Heiko Düssmann, Dr. rer. nat. Director of Molecular Imaging Department of Physiology and Medical Physics RCSI Phone: ++353 1 402 8562 _____________________________________________________________________ Der WEB.DE SmartSurfer hilft bis zu 70% Ihrer Onlinekosten zu sparen! http://smartsurfer.web.de/?mc=100071&distributionid=000000000066 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Do you mean Canon A1 or Nikon F3? I have three A1 bodies. At 08:13 AM 02/01/08, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Hi colleagues >Did anybodyy have hands on the nikon A1? >regards >Heiko > >Heiko Düssmann, Dr. rer. nat. >Director of Molecular Imaging >Department of Physiology and Medical Physics RCSI >Phone: ++353 1 402 8562 > >_____________________________________________________________________ >Der WEB.DE SmartSurfer hilft bis zu 70% Ihrer Onlinekosten zu sparen! >http://smartsurfer.web.de/?mc=100071&distributionid=000000000066 ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/ |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Folks I did get a sneak peak of the new Nikon A1 confocal at the cell biology meeting. It does look very interesting, and has loads of forward looking components... The best thing for all of us is that Nikon have re-entered the market at the top end with a device that looks at first blush that it can provide real competition for oly/leica/zeiss. The things I remember were that it was FAST (in the hundreds of frames/second scale)..and had a hybdrid scanner so you could collect and photoactivate at the same time, another factoid I recall was the ability to collect 32 channels of spectral information at about 16 frames/second... The guys who were showing it stated that there was a big increase in efficiency (I think the number stated was about 30% but I cant be sure). Generally I was very impressed. I know that what I was allowed to see what not the finished product, but these are thing I do remember about the scope. I am sure one of the Nikon marketing folks will tell us the real high points as a response to the original question from Heiko... and of course correct anything I got wrong! We do live in an interesting time with two major new confocals coming out at pretty much the same time! Now who has info on the new zeiss system? S. Simon C. Watkins Ph.D, FRCPath Professor and Vice Chair, Cell Biology and Physiology Professor, Immunology Director, Center for Biologic Imaging BSTS 225, University of Pittsburgh 3500 Terrace St. Pittsburgh PA 15261 Tel: 412-352-2277 Fax:412-648-2797 URL: http://www.cbi.pitt.edu -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michael Cammer Sent: Friday, February 01, 2008 9:15 AM To: [hidden email] Subject: Re: A1 Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Do you mean Canon A1 or Nikon F3? I have three A1 bodies. At 08:13 AM 02/01/08, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Hi colleagues >Did anybodyy have hands on the nikon A1? >regards >Heiko > >Heiko Düssmann, Dr. rer. nat. >Director of Molecular Imaging >Department of Physiology and Medical Physics RCSI >Phone: ++353 1 402 8562 > >_____________________________________________________________________ >Der WEB.DE SmartSurfer hilft bis zu 70% Ihrer Onlinekosten zu sparen! >http://smartsurfer.web.de/?mc=100071&distributionid=000000000066 ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/ |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Simon,
I got a look, too, at ASCB. I certainly concur that it was impressive, and FAST. It reminded me of the speed of the Leica. The software (Elements) controlling it I've seen in earlier form for general scoping, so the notion of the same software backbone for the different Nikon scopes sounds good. The spectral detector system wasn't there that I recall, but I think they said it would ship with systems a bit later. I'm looking forward a thorough look-see soon. Mike *************************** *************************** Michael A. Mancini, Ph.D. Associate Professor Director, Integrated Microscopy Core Co-Director, John S. Dunn GCC for Chemical Genomics Department of Molecular and Cellular Biology Baylor College of Medicine One Baylor Plaza, M803 Houston, TX 77030 713.798.8952.office 713.798.3175.fax 713.408.0179.cell On Feb 1, 2008, at 1:15 PM, Watkins, Simon C wrote:
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In reply to this post by Watkins, Simon C
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear List, I've only read the brochure, but I'd like to add to some of Simon's comments. They claim a 30% increase in sensitivity, this is as I understand it as a result of their hexagonal pinhole. But there are other efficiencies as well which I presume add to this efficiency. I'd like to open these new technologies up for discussion. 1: They compare their variable hexagonal pinhole to a square pinhole found on many confocals. And claim it allows 30% more light though. I guess this depends on your definition. If you open a square pinhole up more you could argue it lets through more light but the resolution is diminished. Nonetheless I'm sure it is a better approximation to the ideal circular pinhole. 2. They use dichroic mirrors at a low angle of incidence instead of the usual 45Deg. They claim also a 30% increase in efficiency here. 3. They use a "Virtual Adaptable Aperture System" (VAAS), the light that a confocal pinhole rejects is collected by another detector. This signal can be deconvolved with the true confocal signal producing a high resolution image but presumably a more sensitive one. This has me really intrigued. It will be interesting to see when there are Refractive Index mismatches in the specimen how this system will compare, I presume RI mis-match may be less destructive with this system. When used in this modality does it act like a scanning wide-field fluorescence microscope with deconvolution? They also include CLEM into the A1 to reduce photobleaching. If the resonant scanner option is purchased (A1R), this is included in parallel to the conventional scanner, and so can be used as a dual scanner. An interesting microscope, I look forward to people's view points on these technologies. Regards Steve Stephen H. Cody Microscopy Manager Central Resource for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Parkville, Victoria, 3050 Australia Tel: 61 3 9341 3155 Fax: 61 3 9341 3104 email: [hidden email] www.ludwig.edu.au/labs/confocal.html www.ludwig.edu.au/confocal Tip: Learn how to receive reminders about you microscope booking: http://www.ludwig.edu.au/confocal/Local/Booking_Hint.htm Type your signature here ________________________________ From: Confocal Microscopy List on behalf of Watkins, Simon C Sent: Sat 02/02/2008 6:15 AM To: [hidden email] Subject: Re: A1 Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Folks I did get a sneak peak of the new Nikon A1 confocal at the cell biology meeting. It does look very interesting, and has loads of forward looking components... The best thing for all of us is that Nikon have re-entered the market at the top end with a device that looks at first blush that it can provide real competition for oly/leica/zeiss. The things I remember were that it was FAST (in the hundreds of frames/second scale)..and had a hybdrid scanner so you could collect and photoactivate at the same time, another factoid I recall was the ability to collect 32 channels of spectral information at about 16 frames/second... The guys who were showing it stated that there was a big increase in efficiency (I think the number stated was about 30% but I cant be sure). Generally I was very impressed. I know that what I was allowed to see what not the finished product, but these are thing I do remember about the scope. I am sure one of the Nikon marketing folks will tell us the real high points as a response to the original question from Heiko... and of course correct anything I got wrong! We do live in an interesting time with two major new confocals coming out at pretty much the same time! Now who has info on the new zeiss system? S. Simon C. Watkins Ph.D, FRCPath Professor and Vice Chair, Cell Biology and Physiology Professor, Immunology Director, Center for Biologic Imaging BSTS 225, University of Pittsburgh 3500 Terrace St. Pittsburgh PA 15261 Tel: 412-352-2277 Fax:412-648-2797 URL: http://www.cbi.pitt.edu <http://www.cbi.pitt.edu/> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michael Cammer Sent: Friday, February 01, 2008 9:15 AM To: [hidden email] Subject: Re: A1 Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Do you mean Canon A1 or Nikon F3? I have three A1 bodies. At 08:13 AM 02/01/08, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Hi colleagues >Did anybodyy have hands on the nikon A1? >regards >Heiko > >Heiko Düssmann, Dr. rer. nat. >Director of Molecular Imaging >Department of Physiology and Medical Physics RCSI >Phone: ++353 1 402 8562 > >_____________________________________________________________________ >Der WEB.DE SmartSurfer hilft bis zu 70% Ihrer Onlinekosten zu sparen! >http://smartsurfer.web.de/?mc=100071&distributionid=000000000066 ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/ |
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