ATTO dyes for live-cell experiments

[Graham Wright-2]

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Hello,

I would like to ask for some opinions/feedback from those with experience
of using ATTO488 and ATTO565 for live-cell imaging experiments (conjugated
to peptides), particularly in terms of photostability/bleaching (planned
for WF and spinning disk microscopy).

I heard from someone that the ATTO565 bleaches relatively quickly (not sure
on their imaging parameters), but would like to hear more. Would people
recommend any other dyes in this spectral range (561 nm laser) that they
have used for these sort of experiments?

Thanks in advance,
Graham

--
Graham Wright, PhD
Head, Microscopy Unit

Institute of Medical Biology, A*STAR
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

W:  http://www.a-star.edu.sg/imb/Tech-Platforms/IMB-Microscopy-Unit.aspx

[lechristophe]

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Hi Graham,

This reference could be useful:

Bosch PJ, Corrêa IR, Sonntag MH, Ibach J, Brunsveld L, Kanger JS &
Subramaniam V (2014) Evaluation of Fluorophores to Label SNAP-Tag Fused
Proteins for Multicolor Single-Molecule Tracking Microscopy in Live
Cells. *Biophys.
J* *107:* 803–814

They have tested 6 green and 16 red dyes for single molecule tracking in
live cells.

Hope this helps,

Christophe




On Fri, Jun 19, 2015 at 10:25 AM, Graham Wright <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> I would like to ask for some opinions/feedback from those with experience
> of using ATTO488 and ATTO565 for live-cell imaging experiments (conjugated
> to peptides), particularly in terms of photostability/bleaching (planned
> for WF and spinning disk microscopy).
>
> I heard from someone that the ATTO565 bleaches relatively quickly (not sure
> on their imaging parameters), but would like to hear more. Would people
> recommend any other dyes in this spectral range (561 nm laser) that they
> have used for these sort of experiments?
>
> Thanks in advance,
> Graham
>
> --
> Graham Wright, PhD
> Head, Microscopy Unit
>
> Institute of Medical Biology, A*STAR
> 8A Biomedical Grove, #06-06 Immunos, Singapore 138648
>
> W:  http://www.a-star.edu.sg/imb/Tech-Platforms/IMB-Microscopy-Unit.aspx
>