ATTO647N and FCS

[Roman Veith]

Dear participants,

I'm using Atto647N as a dye for FCS. Does anyone have experience with
that dye combined with that technique?
I would be really glad if someone could tell me his/her opinion
regarding that dye in FCS concerning measurements in cells!

Greetings,
Roman

--
--
Roman Veith
Diplom-Biologe

Institut für physikalische und
theoretische Chemie
AG Biophysikalische Chemie
Universität Bonn
Wegelerstraße 12
53115 Bonn

Tel-Nr.: 0228-733089
Fax-Nr.: 0228-739424

http://www.thch.uni-bonn.de/pc/kubitscheck/

[Jonathan M Krupp]

Hi:

This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.

I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.

I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?

In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]

[Phil Hertzler]

Jon,

I recommend brine shrimp 3 days old or older, fixed with formalin and stained with rhodamine-phalloidin, which shows up the muscles nicely.  I use this as the first sample to look at in my confocal microscopy class.  Brine shrimp are always available - just add water.  The biggest up front cost in the rhodamine-phalloidin, but you can keep it for years.

Phil Hertzler

At 11:45 AM 11/14/2008, you wrote:

Hi:

This is a pretty basic and simple question. I need some advice about rounding up some samples to use in an elementary light microscopy class that includes basic fluorescence.

I am at a community college and my background is in EM and brightfield LM. I have a passing familiarity with confocal et al but not enough to know much. I maintained a confocal, but never ran experiments or prepared much in the way of samples. Now I have to get something together to demonstrate the fundamentals of fluorescence to students in a new job.

I have been relying on chlorophyll autofluorescence up til now, but would like to add anything that would be easy to do. We have a simple scope with filters for FITC, rhodamine, and DAPI, I think. What would be some fool proof, easy to get samples to try?

In addition, I would eventually like to add some kind of confocal experience to this class, any ideas about where to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]

---

Philip L. Hertzler
Associate Professor
Central Michigan University
Dept. of Biology, Brooks Hall 217
Mount Pleasant, MI 48859

Phone: [phone]
Fax: [phone]
Email: [hidden email]

"Teachers should be paid more."
- Sarah Palin, Vice-presidential debate, 10/2/2008

"It is common sense to take a method and try it. If it fails, admit it frankly and try another. But above all, try something."
- Franklin D. Roosevelt

"Not everything that counts can be counted, and not everything that can be counted counts."
- attributed to Albert Einstein

[cromey]

To teach new users the basics of how our confocal works (before we get to
working with their samples) I use a kimwipe that has been marked with
several different fluorescent highlighters.  I mount a piece of the kimwipe
to a slide with permount under a #1.5 coverslip and it lasts almost forever.
It's incredibly bright, moderately thick (~90um), cheap to make, and 3D
rotations using a 10x objective show the fibers very nicely.  This teaching
slide idea was inspired by my colleagues "up the road" at AZ State
University with their Paper project < http://paperproject.org/>.

The kimwipe slide is not very effective to show two different wavelengths,
but it lets me talk about spectral bleedthrough/artifactual colocalization
issues and why the longer wavelength channel is usually noisier (PMT gain is
often twice that of the shorter wavelength).

The Invitrogen Fluocells slides (no commercial interest) are beautiful for
demos, but they are expensive.

H&E stained histologic sections fluoresce and I've been told that diatoms
(good for demonstrating DIC if your confocal has all the parts for it) are
autofluorescent.

Have fun.
Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

office:  AHSC 4212         email: [hidden email]
voice:  520-626-2824       fax:  [phone]

http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Jonathan M Krupp
Sent: Friday, November 14, 2008 9:46 AM
To: [hidden email]
Subject: Practice samples

Hi:

This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.

I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.

I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?

In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]

[Tamara Howard]

I saw that someone mentioned H&E stained sections - eosin
does fluoresce nicely under the filters you have (well,
not so much with the DAPI). If you can't get actual
stained tissue slices, you can just mix any fluorescent
dye into mounting media. Some plastics fluoresce;
 Kimwipes are great, too - although it never occurred to
me to make a permanent mount (I'm going to do that this
morning). if you check the archives there was a discussion
about making pollen slides earlier this year - those will
fluoresce & can be done "on the cheap". Carolina
Biological sells permanent slides of all kinds of things;
there are other biological supply places but I can't think
of any right now.

Tamara

On Fri, 14 Nov 2008 08:45:52 -0800
  Jonathan M Krupp <[hidden email]> wrote:

> Hi:
>
> This is a pretty basic and simple question. I need some
>advice about rounding up some samples to use in an
>elementary light microscopy class that includes basic
>fluorescence.
>
> I am at a community college and my background is in EM
>and brightfield LM. I have a passing familiarity with
>confocal et al but not enough to know much. I maintained
>a confocal, but never ran experiments or prepared much in
>the way of samples. Now I have to get something together
>to demonstrate the fundamentals of fluorescence to
>students in a new job.
>
> I have been relying on chlorophyll autofluorescence up
>til now, but would like to add anything that would be
>easy to do. We have a simple scope with filters for FITC,
>rhodamine, and DAPI, I think. What would be some fool
>proof, easy to get samples to try?
>
> In addition, I would eventually like to add some kind of
>confocal experience to this class, any ideas about where
>to find an inexpensive system would be great.
>
> Thanks
>
> Jon
> San Joaquin Delta College
> Stockton, CA 95207
> [hidden email]

***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

[Torsten.Fregin]

I think some CLSM vendors use Convallaria root sections as test preparations for their
scopes and also sell them.

:-) Torsten

[Armstrong, Brian]

Hi Jon, the pollen grains will fluoresce nicely and are cheap and easy
to make. You can get plastic fluorescent slides from Chroma for free
which are good for maintenance/QC. In my opinion the Molecular Probes
slides are expensive, but well worth it. I have imaged that slide a
thousand times! It always gets a "wow" from the trainee. Pollen grains
are boring! (unless maybe you explode them with your 2P laser).

We have a Zeiss LSM310 Confocal for sale! Let me know if you want to
discuss the particulars.
Cheers,

Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag
ing/Pages/default.aspx

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Jonathan M Krupp
Sent: Friday, November 14, 2008 8:46 AM
To: [hidden email]
Subject: Practice samples

Hi:

This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.

I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.

I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?

In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]


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[Mollie Lange]

Jonathan,

 

You can order all kinds of prepared fluorescent slides from Molecular Probes/Invitrogen.

>>> Jonathan M Krupp <[hidden email]> 11/14/2008 11:45 AM >>>
Hi:

This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.

I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.

I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?

In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]

Please consider the environment when printing this E-Mail.

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[Glen MacDonald-2]

Has anyone labeled live brine shrimp with vital stains for practice  
specimens?

Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

******************************************************************************
The box said "Requires WindowsXP or better", so I bought a Macintosh.
******************************************************************************


On Nov 14, 2008, at 9:00 AM, Phil Hertzler wrote:

> Jon,
>
> I recommend brine shrimp 3 days old or older, fixed with formalin  
> and stained with rhodamine-phalloidin, which shows up the muscles  
> nicely.  I use this as the first sample to look at in my confocal  
> microscopy class.  Brine shrimp are always available - just add  
> water.  The biggest up front cost in the rhodamine-phalloidin, but  
> you can keep it for years.
>
> Phil Hertzler
>
> At 11:45 AM 11/14/2008, you wrote:
>> Hi:
>>
>> This is a pretty basic and simple question. I need some advice  
>> about rounding up some samples to use in an elementary light  
>> microscopy class that includes basic fluorescence.
>>
>> I am at a community college and my background is in EM and  
>> brightfield LM. I have a passing familiarity with confocal et al  
>> but not enough to know much. I maintained a confocal, but never ran  
>> experiments or prepared much in the way of samples. Now I have to  
>> get something together to demonstrate the fundamentals of  
>> fluorescence to students in a new job.
>>
>> I have been relying on chlorophyll autofluorescence up til now, but  
>> would like to add anything that would be easy to do. We have a  
>> simple scope with filters for FITC, rhodamine, and DAPI, I think.  
>> What would be some fool proof, easy to get samples to try?
>>
>> In addition, I would eventually like to add some kind of confocal  
>> experience to this class, any ideas about where to find an  
>> inexpensive system would be great.
>>
>> Thanks
>>
>> Jon
>> San Joaquin Delta College
>> Stockton, CA 95207
>> [hidden email]
>
> Philip L. Hertzler
> Associate Professor
> Central Michigan University
> Dept. of Biology, Brooks Hall 217
> Mount Pleasant, MI 48859
>
> Phone: [phone]
> Fax: [phone]
> Email: [hidden email]
>
> "Teachers should be paid more."
> - Sarah Palin, Vice-presidential debate, 10/2/2008
>
> "It is common sense to take a method and try it. If it fails, admit  
> it frankly and try another. But above all, try something."
> - Franklin D. Roosevelt
>
> "Not everything that counts can be counted, and not everything that  
> can be counted counts."
> - attributed to Albert Einstein

[Tobias Baskin]

Jon,
        Plants are full of autofluorescence. If you cut a section (a
careful hand section is fine) of a stem or root, most times you will
see lovely fluorescence. What is nice about this is that you can have
students collect their own matieral, try different places on the
plant, aged material, etc etc. It takes a little knack to cut nice
smooth hand sections so they coverslip up nice and show good images
near the coverslip. You don't have to stain at all, just mount and
observe. There can also be nice differences at different wavelengths.
        Have fun,
                Tobias


At 8:45 AM -0800 11/14/08, Jonathan M Krupp wrote:

>Hi:
>
>This is a pretty basic and simple question. I need some advice about
>rounding up some samples to use in an elementary light microscopy
>class that includes basic fluorescence.
>
>I am at a community college and my background is in EM and
>brightfield LM. I have a passing familiarity with confocal et al but
>not enough to know much. I maintained a confocal, but never ran
>experiments or prepared much in the way of samples. Now I have to
>get something together to demonstrate the fundamentals of
>fluorescence to students in a new job.
>
>I have been relying on chlorophyll autofluorescence up til now, but
>would like to add anything that would be easy to do. We have a
>simple scope with filters for FITC, rhodamine, and DAPI, I think.
>What would be some fool proof, easy to get samples to try?
>
>In addition, I would eventually like to add some kind of confocal
>experience to this class, any ideas about where to find an
>inexpensive system would be great.
>
>Thanks
>
>Jon
>San Joaquin Delta College
>Stockton, CA 95207
>[hidden email]

--
       _      ____          __   ____  
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of Massachusetts
  /      /          /         \   \       \    Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

[Patrick Van Oostveldt]

Dear Jon,

A lot of house hold products show very bride fluorescence. If you take  
a white T-shirt, the whitener in the washing powder will make the  
contton fibers bride fluorescent for UV.
Most textile dyes are fluorescent also, so you can have choice.

Pollen from plants are also fluorescent. Bye e.g. lilies, give them to  
your love, but take the pollen of before. This will also improve the  
shelf-live of your flowers when put in water. To prepare these pollen,  
let them dry, and mount in immersion oil or non fluorescent glycerine.

Finally the paper of euro's and also very frequently some stamps  
fluoresce. You can check that by illuminating them with blacklight as  
used in the disco.

It is amazing to test all different products, because even the human  
hair, washed with colored shampoo, will show autofluorescence coming  
from the dye in the shampoo, on the other hand, hair from wild animals  
will in general not fluoresce.

Bye,

Patrick
Dep. Molecular Biotechnology
Ghent University
Coupure links 653
9000 GENT (Belgium)

Quoting Jonathan M Krupp <[hidden email]>:

> Hi:
>
> This is a pretty basic and simple question. I need some advice about
> rounding up some samples to use in an elementary light microscopy class
> that includes basic fluorescence.
>
> I am at a community college and my background is in EM and brightfield
> LM. I have a passing familiarity with confocal et al but not enough to
> know much. I maintained a confocal, but never ran experiments or
> prepared much in the way of samples. Now I have to get something
> together to demonstrate the fundamentals of fluorescence to students in
> a new job.
>
> I have been relying on chlorophyll autofluorescence up til now, but
> would like to add anything that would be easy to do. We have a simple
> scope with filters for FITC, rhodamine, and DAPI, I think. What would
> be some fool proof, easy to get samples to try?
>
> In addition, I would eventually like to add some kind of confocal
> experience to this class, any ideas about where to find an inexpensive
> system would be great.
>
> Thanks
>
> Jon
> San Joaquin Delta College
> Stockton, CA 95207
> [hidden email]

--
Dep. Moleculaire Biotechnologie
Coupure links 653
B 9000 GENT

tel [phone]
fax [phone]

[Klughammer Industrie GmbH]

Dear Jon,

why don't you use curry powder? It has nice fluorescence which never bleaches.

Anneliese Schmaus
Klughammer Bio GmbH



2008/11/15 Patrick Van Oostveldt <[hidden email]>:

> Dear Jon,
>
> A lot of house hold products show very bride fluorescence. If you take a
> white T-shirt, the whitener in the washing powder will make the contton
> fibers bride fluorescent for UV.
> Most textile dyes are fluorescent also, so you can have choice.
>
> Pollen from plants are also fluorescent. Bye e.g. lilies, give them to your
> love, but take the pollen of before. This will also improve the shelf-live
> of your flowers when put in water. To prepare these pollen, let them dry,
> and mount in immersion oil or non fluorescent glycerine.
>
> Finally the paper of euro's and also very frequently some stamps fluoresce.
> You can check that by illuminating them with blacklight as used in the
> disco.
>
> It is amazing to test all different products, because even the human hair,
> washed with colored shampoo, will show autofluorescence coming from the dye
> in the shampoo, on the other hand, hair from wild animals will in general
> not fluoresce.
>
> Bye,
>
> Patrick
> Dep. Molecular Biotechnology
> Ghent University
> Coupure links 653
> 9000 GENT (Belgium)
>
> Quoting Jonathan M Krupp <[hidden email]>:
>
>> Hi:
>>
>> This is a pretty basic and simple question. I need some advice about
>> rounding up some samples to use in an elementary light microscopy class
>> that includes basic fluorescence.
>>
>> I am at a community college and my background is in EM and brightfield
>> LM. I have a passing familiarity with confocal et al but not enough to
>> know much. I maintained a confocal, but never ran experiments or
>> prepared much in the way of samples. Now I have to get something
>> together to demonstrate the fundamentals of fluorescence to students in
>> a new job.
>>
>> I have been relying on chlorophyll autofluorescence up til now, but
>> would like to add anything that would be easy to do. We have a simple
>> scope with filters for FITC, rhodamine, and DAPI, I think. What would
>> be some fool proof, easy to get samples to try?
>>
>> In addition, I would eventually like to add some kind of confocal
>> experience to this class, any ideas about where to find an inexpensive
>> system would be great.
>>
>> Thanks
>>
>> Jon
>> San Joaquin Delta College
>> Stockton, CA 95207
>> [hidden email]
>
>
>
> --
> Dep. Moleculaire Biotechnologie
> Coupure links 653
> B 9000 GENT
>
> tel [phone]
> fax [phone]
>

[J. Paul Robinson]

Jon
You may find the confocal/imaging class lectures I give here at Purdue
useful for your course. You can find the class lectures at
http://www.cyto.purdue.edu/class/bms524/index.htm

and more at
http://www.cyto.purdue.edu/flowcyt/educate/pptslide.htm

some were recorded using Adobe connect
all have the original PPTs there to download which you may find useful.
We also have a practical class - if you would like that materials you
could email me directly

kind regards

paul



Jonathan M Krupp wrote:

> Hi:
>
> This is a pretty basic and simple question. I need some advice about
> rounding up some samples to use in an elementary light microscopy class
> that includes basic fluorescence.
>
> I am at a community college and my background is in EM and brightfield
> LM. I have a passing familiarity with confocal et al but not enough to
> know much. I maintained a confocal, but never ran experiments or
> prepared much in the way of samples. Now I have to get something
> together to demonstrate the fundamentals of fluorescence to students in
> a new job.
>
> I have been relying on chlorophyll autofluorescence up til now, but
> would like to add anything that would be easy to do. We have a simple
> scope with filters for FITC, rhodamine, and DAPI, I think. What would be
> some fool proof, easy to get samples to try?
>
> In addition, I would eventually like to add some kind of confocal
> experience to this class, any ideas about where to find an inexpensive
> system would be great.
>
> Thanks
>
> Jon
> San Joaquin Delta College
> Stockton, CA 95207
> [hidden email]

--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
Past-President, International Society for Analytical Cytology
(now International Society for Advancement of Cytometry)

Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph [phone]; Fax [phone]
email: [hidden email]
www.cyto.purdue.edu

Track Paul's climb in the Himalayas September 2008
http://www.cyto.purdue.edu/trackpaul/
and see the Manaslu photos at
http://picasaweb.google.com/climbingabc

Join ISAC - www.isac-net.org
Change lives today  - www.cytometryforlife.org

[Rosemary.White]

Hi Jon,
As Tobias says, plants are great for their variety of autofluorescence.  I
cut hand sections (from a couple of bushes just outside the building) in
front of school groups and put them straight under the fluorescence or
confocal microscope.  UV/405nm illumination will give a big variety of
fluorescence from cell walls and cell contents.  There are also cheap stains
like rhodamine B, berberine sulfate, Schiff's reagent, calcofluor white (the
brightener in washing powder) that you can use, which mainly stain cell
walls.
Another cool thing to do is get a cube of either uranium glass or
fluorescent plastic and put this under the objective to show the light path
and explain the focal plane - or fill a cuvette with fluorescent solution.
Does a much better job than diagrams on a piece of paper!
cheers,
Rosemary

On 15/11/08 5:56 PM, "Tobias Baskin" <[hidden email]> wrote:

> Jon,
>         Plants are full of autofluorescence. If you cut a section (a
> careful hand section is fine) of a stem or root, most times you will
> see lovely fluorescence. What is nice about this is that you can have
> students collect their own matieral, try different places on the
> plant, aged material, etc etc. It takes a little knack to cut nice
> smooth hand sections so they coverslip up nice and show good images
> near the coverslip. You don't have to stain at all, just mount and
> observe. There can also be nice differences at different wavelengths.
>         Have fun,
>                 Tobias
>
>
> At 8:45 AM -0800 11/14/08, Jonathan M Krupp wrote:
>> Hi:
>>
>> This is a pretty basic and simple question. I need some advice about
>> rounding up some samples to use in an elementary light microscopy
>> class that includes basic fluorescence.
>>
>> I am at a community college and my background is in EM and
>> brightfield LM. I have a passing familiarity with confocal et al but
>> not enough to know much. I maintained a confocal, but never ran
>> experiments or prepared much in the way of samples. Now I have to
>> get something together to demonstrate the fundamentals of
>> fluorescence to students in a new job.
>>
>> I have been relying on chlorophyll autofluorescence up til now, but
>> would like to add anything that would be easy to do. We have a
>> simple scope with filters for FITC, rhodamine, and DAPI, I think.
>> What would be some fool proof, easy to get samples to try?
>>
>> In addition, I would eventually like to add some kind of confocal
>> experience to this class, any ideas about where to find an
>> inexpensive system would be great.
>>
>> Thanks
>>
>> Jon
>> San Joaquin Delta College
>> Stockton, CA 95207
>> [hidden email]
>
>
> --
>        _      ____          __   ____
>       /  \   /          / \    /   \ \        Tobias I. Baskin
>      /   /  /          /   \   \      \         Biology Department
>     /_ /   __      /__ \   \       \__    611 N. Pleasant St.
>    /      /          /       \   \       \        University of Massachusetts
>   /      /          /         \   \       \         Amherst, MA, 01003
> /      / ___   /           \   \__/  \ ____
> www.bio.umass.edu/biology/baskin
> Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

[Grace Chojnowski-2]

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We have a new position in our facility for a microscopist to join our dynamic team in the Flow Cytometry and Imaging Facility at QIMR Berghofer Research Institute. For more details please go to :-

http://www.qimrberghofer.edu.au/page/Careers/Positions_vacant/ 
Reference Number 11624  


Grace Chojnowski  |  Flow Cytometry and Imaging Facility Manager QIMR Berghofer Medical Research Institute
 
t [phone]  |  m [phone]  |   f [phone] e [hidden email]  |  www.qimr.edu.au
300 Herston Road, Herston QLD 4006
Locked Bag 2000 Royal Brisbane Hospital, QLD 4029