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Hi all you microscope wizards,
I am trying to bring an ablation laser into a Nikon E800 (upright) via a combiner
on the epi illumination pathway. I am having a hard time finding out exactly
what the lenses in this pathway do. When I put in the laser beam expanded to
10mm and 0 convergence I am nowhere near image focus (as expected).
When I use the laser beam expander to focus the laser beam to the image focus
(60X 1.4n superfluous), then the laser beam size at the objective back aperture
is only 4mm. My question is whether I can simply put an additional 5X beam
expander in the laser beam pathway just before it enters the combiner and get
a 20 mm beam diameter at the objective back aperture and the laser beam
focus congruent with the image focus.
My other question is whether anyone has tried to remove all the lenses in the
intermediate piece of a E800. I can get the first two off with the illuminator
mount, then I am stuck at a large rectangular gray metal plate with two
additional lenses mounted. There are two large bolts on the sides of this plate,
but removing them does not free up the plate.
thanks for any help,
michael
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