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Acceptor Photobleaching vs. Sensitized Emission FRET results

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Robin Battye

Re: Cheap or free sources of cells

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Hi John,

Perhaps you can look at Keratocytes. A goldfish in a beer mug is a good source.

You can contact me offline or I will see you at Spectral.

Cheers Robin

Robin Battye, M.Sc., Ph.D.
Vice President Sales and Development
[hidden email]
www.quorumtechnologies.com
Cell: (647) 285-9998
Office: (519) 824-0854
Fax: (519) 824-5845

On 2010-10-14, at 7:03, John Oreopoulos <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal listserver,
>
> I'm looking for cheap or free sources of cells that can be used for testing various fluorescent stains and/or microscopy instrumentation. I've had good success in the past with blood cells pricked from my finger with a finger lance and cheek cells scraped from my mouth. I'm wondering if any of you out there know of any other sources of cells for this same kind of purpose. Maybe urine samples? Anything from nature/outdoors (besides pond water)? Anything from the supermarket?
>
> Looking forward to all your creative responses!
>
> John Oreopoulos

Baggi Somasundaram

Re: Cheap or free sources of cells

In reply to this post by John Oreopoulos

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Hi John
The use of onion cells for live cell confocal demonstrations always works well.
Link
http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=onion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhCNafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=result&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20confocal&f=false

Baggi Somasundaram
BioImaging Solutions
[hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Oreopoulos
Sent: Thursday, October 14, 2010 7:04 AM
To: [hidden email]
Subject: Cheap or free sources of cells

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear confocal listserver,

I'm looking for cheap or free sources of cells that can be used for testing various fluorescent stains and/or microscopy instrumentation. I've had good success in the past with blood cells pricked from my finger with a finger lance and cheek cells scraped from my mouth. I'm wondering if any of you out there know of any other sources of cells for this same kind of purpose. Maybe urine samples? Anything from nature/outdoors (besides pond water)? Anything from the supermarket?

Looking forward to all your creative responses!

John Oreopoulos

John Oreopoulos

Re: Cheap or free sources of cells

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*****

Yes, the onion! I had forgotten about that one. And this preparation by Pawley is a good one since one of the dyes I wanted to test is DiR (instead of DiO).

Any more ideas out there? Plant, yeast, and fungi cells are good, but are there any more animal cell sources?

Thanks for all replies so far.

John Oreopoulos

On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi John
> The use of onion cells for live cell confocal demonstrations always works well.
> Link
> http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=onion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhCNafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=result&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20confocal&f=false
>
> Baggi Somasundaram
> BioImaging Solutions
> [hidden email]
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Oreopoulos
> Sent: Thursday, October 14, 2010 7:04 AM
> To: [hidden email]
> Subject: Cheap or free sources of cells
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal listserver,
>
> I'm looking for cheap or free sources of cells that can be used for testing various fluorescent stains and/or microscopy instrumentation. I've had good success in the past with blood cells pricked from my finger with a finger lance and cheek cells scraped from my mouth. I'm wondering if any of you out there know of any other sources of cells for this same kind of purpose. Maybe urine samples? Anything from nature/outdoors (besides pond water)? Anything from the supermarket?
>
> Looking forward to all your creative responses!
>
> John Oreopoulos

Oshel, Philip Eugene

Re: Cheap or free sources of cells

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*****

How about the old classroom standby: epithelial cells from cheek swabs?
Phil

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Yes, the onion! I had forgotten about that one. And this preparation
>by Pawley is a good one since one of the dyes I wanted to test is
>DiR (instead of DiO).
>
>Any more ideas out there? Plant, yeast, and fungi cells are good,
>but are there any more animal cell sources?
>
>Thanks for all replies so far.
>
>John Oreopoulos
>
>
>
>On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi John
>> The use of onion cells for live cell confocal demonstrations
>>always works well.
>> Link
>>
>>http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=onion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhCNafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=result&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20confocal&f=false
>>
>> Baggi Somasundaram
>> BioImaging Solutions
>> [hidden email]
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>>[mailto:[hidden email]] On Behalf Of John
>>Oreopoulos
>> Sent: Thursday, October 14, 2010 7:04 AM
>> To: [hidden email]
>> Subject: Cheap or free sources of cells
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear confocal listserver,
>>
>> I'm looking for cheap or free sources of cells that can be used
>>for testing various fluorescent stains and/or microscopy
>>instrumentation. I've had good success in the past with blood cells
>>pricked from my finger with a finger lance and cheek cells scraped
>>from my mouth. I'm wondering if any of you out there know of any
>>other sources of cells for this same kind of purpose. Maybe urine
>>samples? Anything from nature/outdoors (besides pond water)?
>>Anything from the supermarket?
>>
>> Looking forward to all your creative responses!
>>
>> John Oreopoulos

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

Julio Vazquez

Re: Cheap or free sources of cells

In reply to this post by John Oreopoulos

*****
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*****

-
I feel a bit embarrassed about this one, but one of the earliest cell
samples to be seen under the scope by Leeuwenhoek were spematozoa.
Apparently, this created some issues between him and the Dutch
clergy. Things may not have changed much in some places, so I can not
wholeheartedly recommend it...
--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024

http://www.fhcrc.org/

On Oct 14, 2010, at 8:26 AM, John Oreopoulos wrote:

> Yes, the onion! I had forgotten about that one. And this
> preparation by Pawley is a good one since one of the dyes I wanted
> to test is DiR (instead of DiO).
>
> Any more ideas out there? Plant, yeast, and fungi cells are good,
> but are there any more animal cell sources?
>
> Thanks for all replies so far.
>
> John Oreopoulos

Judy Trogadis-2

Re: Cheap or free sources of cells

In reply to this post by Oshel, Philip Eugene

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*****

Just on my way back from the Woods Hole course and learned that not only are cheek cells free, abundant (at least in females, men shed fewer ones, reason unknown), great for testing, calibrating all the different brightfield modalities, but they are also autofluorescent.

But guys, be diplomatic when you ask your female colleagues for a sample. ;-)
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-5046
[hidden email]

>>> Philip Oshel <[hidden email]> 10/14/2010 12:32 PM >>>
*****
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*****

How about the old classroom standby: epithelial cells from cheek swabs?
Phil

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

Judy Trogadis-2

Re: Cheap or free sources of cells

In reply to this post by John Oreopoulos

*****
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*****

Sorry, didn't read the first email in the thread. Cheek cells would definitely NOT be appropriate because of the inherent autofluorescence . That may be true for other common sources of material within our daily environment.
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-5046
[hidden email]

>>> John Oreopoulos <[hidden email]> 10/14/2010 10:03 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Dear confocal listserver,

I'm looking for cheap or free sources of cells that can be used for testing various fluorescent stains and/or microscopy instrumentation. I've had good success in the past with blood cells pricked from my finger with a finger lance and cheek cells scraped from my mouth. I'm wondering if any of you out there know of any other sources of cells for this same kind of purpose. Maybe urine samples? Anything from nature/outdoors (besides pond water)? Anything from the supermarket?

Looking forward to all your creative responses!

John Oreopoulos

Glen MacDonald-2

Re: Cheap or free sources of cells

In reply to this post by John Oreopoulos

*****
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Hi John,
Hair bulbs and fish scale keratinocytes are easy. You might find labs that occasionally have extra larvae from sources such as zebrafish or xenopus, although they generally don't take up AM esters. Fresh bones from the butcher shop can have live chondrocytes within the cartilage. clams and oysters can provide several different tissue types.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]

On Oct 14, 2010, at 7:03 AM, John Oreopoulos wrote:

Ignatius, Mike-2

Re: Cheap or free sources of cells

In reply to this post by Oshel, Philip Eugene

*****
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*****

The nice thing about cheek swab is that you get lots of bacteria as well, which can be used for quick demo.

Make the cheek swab prep, then image live vs. dead bacteria with our fluorescent LIVE/DEAD(r) BacLight(tm) Bacterial Viability Kit L7012.

Then gargle with either alcohol based or cetylpyridinium chloride (CPC) based mouthwash. Scrap some more cheek cells and see the dramatic increase in dead (red) bacteria.

Mike Ignatius

Molecular Probes/Life Technologies

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Philip Oshel
Sent: Thursday, October 14, 2010 9:33 AM
To: [hidden email]
Subject: Re: Cheap or free sources of cells

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

How about the old classroom standby: epithelial cells from cheek swabs?
Phil

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

Achille Dunne

Re: Cheap or free sources of cells

In reply to this post by Julio Vazquez

*****
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*****

I wouldn't recommend this approach if it's for a school classroom
demonstration!

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Julio Vazquez
Sent: 14 October 2010 17:54
To: [hidden email]
Subject: Re: Cheap or free sources of cells

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

-
I feel a bit embarrassed about this one, but one of the earliest cell
samples to be seen under the scope by Leeuwenhoek were spematozoa.
Apparently, this created some issues between him and the Dutch clergy.
Things may not have changed much in some places, so I can not
wholeheartedly recommend it...
--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024

http://www.fhcrc.org/

On Oct 14, 2010, at 8:26 AM, John Oreopoulos wrote:

> Yes, the onion! I had forgotten about that one. And this preparation
> by Pawley is a good one since one of the dyes I wanted to test is DiR
> (instead of DiO).
>
> Any more ideas out there? Plant, yeast, and fungi cells are good, but
> are there any more animal cell sources?
>
> Thanks for all replies so far.
>
> John Oreopoulos

--------------------------------------------------------
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JOEL B. SHEFFIELD

Re: Cheap or free sources of cells

In reply to this post by Ignatius, Mike-2

*****
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*****

I suppose you can expand this demo by taking a later swab and seeing how
long it takes to replace the dead with the living.

Joel

On Thu, Oct 14, 2010 at 4:25 PM, Ignatius, Mike
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> The nice thing about cheek swab is that you get lots of bacteria as well,
> which can be used for quick demo.
>
> Make the cheek swab prep, then image live vs. dead bacteria with our
> fluorescent LIVE/DEAD(r) BacLight(tm) Bacterial Viability Kit L7012.
>
> Then gargle with either alcohol based or cetylpyridinium chloride (CPC)
> based mouthwash. Scrap some more cheek cells and see the dramatic increase
> in dead (red) bacteria.
>
> Mike Ignatius
>
> Molecular Probes/Life Technologies
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Philip Oshel
> Sent: Thursday, October 14, 2010 9:33 AM
> To: [hidden email]
> Subject: Re: Cheap or free sources of cells
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> How about the old classroom standby: epithelial cells from cheek swabs?
> Phil
>
> >*****
> >To join, leave or search the confocal microscopy listserv, go to:
> >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >*****
> >
> >Yes, the onion! I had forgotten about that one. And this preparation
> >by Pawley is a good one since one of the dyes I wanted to test is
> >DiR (instead of DiO).
> >
> >Any more ideas out there? Plant, yeast, and fungi cells are good,
> >but are there any more animal cell sources?
> >
> >Thanks for all replies so far.
> >
> >John Oreopoulos
> >
> >
> >
> >On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Hi John
> >> The use of onion cells for live cell confocal demonstrations
> >>always works well.
> >> Link
> >>
> >>
> http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=onion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhCNafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=result&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20confocal&f=false
> >>
> >> Baggi Somasundaram
> >> BioImaging Solutions
> >> [hidden email]
> >>
> >> -----Original Message-----
> >> From: Confocal Microscopy List
> >>[mailto:[hidden email]] On Behalf Of John
> >>Oreopoulos
> >> Sent: Thursday, October 14, 2010 7:04 AM
> >> To: [hidden email]
> >> Subject: Cheap or free sources of cells
> >>
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Dear confocal listserver,
> >>
> >> I'm looking for cheap or free sources of cells that can be used
> >>for testing various fluorescent stains and/or microscopy
> >>instrumentation. I've had good success in the past with blood cells
> >>pricked from my finger with a finger lance and cheek cells scraped
> >>from my mouth. I'm wondering if any of you out there know of any
> >>other sources of cells for this same kind of purpose. Maybe urine
> >>samples? Anything from nature/outdoors (besides pond water)?
> >>Anything from the supermarket?
> >>
> >> Looking forward to all your creative responses!
> >>
> >> John Oreopoulos
>
> --
> Philip Oshel
> Microscopy Facility Supervisor
> Biology Department
> 024C Brooks Hall
> Central Michigan University
> Mt. Pleasant, MI 48859
> (989) 774-3576
>

--

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL: http://astro.temple.edu/~jbs

Eric Scarfone

Re: Cheap or free sources of cells

*****
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*****

Hi there
If you are brave enough you could also try this approach:
Technique to Collect Fungiform (Taste) Papillae from Human Tongue
Andrew I. Spielman et al.
J Vis Exp. 2010 Sep 18;(42). pii: 2201. doi: 10.3791/2201.

Incidentaly I have stumbled by chance on this jounal but it is a gem!
Good luck
Eric A. Scarfone

(ps: Warning, I have not tried it myself, so ....at your own risk.)
(pps: now that I am thinking about it there are people using laser scanning techniques for non-invasive imaging under biological surfaces (ie. Skin) but I am not if the short and long term consequences have been fully investigated!)

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876

email: [hidden email]
http://www.ki.se/cfh/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of JOEL B. SHEFFIELD
Sent: 16 October 2010 19:00
To: [hidden email]
Subject: Re: Cheap or free sources of cells

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I suppose you can expand this demo by taking a later swab and seeing how long it takes to replace the dead with the living.

Joel

On Thu, Oct 14, 2010 at 4:25 PM, Ignatius, Mike
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> The nice thing about cheek swab is that you get lots of bacteria as
> well, which can be used for quick demo.
>
> Make the cheek swab prep, then image live vs. dead bacteria with our
> fluorescent LIVE/DEAD(r) BacLight(tm) Bacterial Viability Kit L7012.
>
> Then gargle with either alcohol based or cetylpyridinium chloride
> (CPC) based mouthwash. Scrap some more cheek cells and see the
> dramatic increase in dead (red) bacteria.
>
> Mike Ignatius
>
> Molecular Probes/Life Technologies
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On Behalf Of Philip Oshel
> Sent: Thursday, October 14, 2010 9:33 AM
> To: [hidden email]
> Subject: Re: Cheap or free sources of cells
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> How about the old classroom standby: epithelial cells from cheek swabs?
> Phil
>
> >*****
> >To join, leave or search the confocal microscopy listserv, go to:
> >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >*****
> >
> >Yes, the onion! I had forgotten about that one. And this preparation
> >by Pawley is a good one since one of the dyes I wanted to test is DiR
> >(instead of DiO).
> >
> >Any more ideas out there? Plant, yeast, and fungi cells are good, but
> >are there any more animal cell sources?
> >
> >Thanks for all replies so far.
> >
> >John Oreopoulos
> >
> >
> >
> >On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Hi John
> >> The use of onion cells for live cell confocal demonstrations
> >>always works well.
> >> Link
> >>
> >>
> http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=on
> ion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhC
> Nafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=resul
> t&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20con
> focal&f=false
> >>
> >> Baggi Somasundaram
> >> BioImaging Solutions
> >> [hidden email]
> >>
> >> -----Original Message-----
> >> From: Confocal Microscopy List
> >>[mailto:[hidden email]] On Behalf Of John
> >>Oreopoulos
> >> Sent: Thursday, October 14, 2010 7:04 AM
> >> To: [hidden email]
> >> Subject: Cheap or free sources of cells
> >>
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Dear confocal listserver,
> >>
> >> I'm looking for cheap or free sources of cells that can be used
> >>for testing various fluorescent stains and/or microscopy
> >>instrumentation. I've had good success in the past with blood cells
> >>pricked from my finger with a finger lance and cheek cells scraped
> >>from my mouth. I'm wondering if any of you out there know of any
> >>other sources of cells for this same kind of purpose. Maybe urine
> >>samples? Anything from nature/outdoors (besides pond water)?
> >>Anything from the supermarket?
> >>
> >> Looking forward to all your creative responses!
> >>
> >> John Oreopoulos
>
> --
> Philip Oshel
> Microscopy Facility Supervisor
> Biology Department
> 024C Brooks Hall
> Central Michigan University
> Mt. Pleasant, MI 48859
> (989) 774-3576
>

--

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL: http://astro.temple.edu/~jbs

Craig Brideau

Re: Cheap or free sources of cells

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

The Dermainspect system in Europe is basically a 2 photon microscope with
special gear to allow it to image skin on a person. The goal was to check
moles/freckles for signs of cancer.
http://www.jenlab.de/DermaInspect-R.29.0.html

(no commercial relationship)

Craig

> (pps: now that I am thinking about it there are people using laser scanning
> techniques for non-invasive imaging under biological surfaces (ie. Skin) but
> I am not if the short and long term consequences have been fully
> investigated!)
>
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: +46 (0)8-517 79343,
> Cell: +46 (0)70 888 2352
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>
> email: [hidden email]
> http://www.ki.se/cfh/
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of JOEL B. SHEFFIELD
> Sent: 16 October 2010 19:00
> To: [hidden email]
> Subject: Re: Cheap or free sources of cells
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I suppose you can expand this demo by taking a later swab and seeing how
> long it takes to replace the dead with the living.
>
> Joel
>
>
> On Thu, Oct 14, 2010 at 4:25 PM, Ignatius, Mike
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > The nice thing about cheek swab is that you get lots of bacteria as
> > well, which can be used for quick demo.
> >
> > Make the cheek swab prep, then image live vs. dead bacteria with our
> > fluorescent LIVE/DEAD(r) BacLight(tm) Bacterial Viability Kit L7012.
> >
> > Then gargle with either alcohol based or cetylpyridinium chloride
> > (CPC) based mouthwash. Scrap some more cheek cells and see the
> > dramatic increase in dead (red) bacteria.
> >
> > Mike Ignatius
> >
> > Molecular Probes/Life Technologies
> >
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[hidden email]]
> > On Behalf Of Philip Oshel
> > Sent: Thursday, October 14, 2010 9:33 AM
> > To: [hidden email]
> > Subject: Re: Cheap or free sources of cells
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > How about the old classroom standby: epithelial cells from cheek swabs?
> > Phil
> >
> > >*****
> > >To join, leave or search the confocal microscopy listserv, go to:
> > >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >*****
> > >
> > >Yes, the onion! I had forgotten about that one. And this preparation
> > >by Pawley is a good one since one of the dyes I wanted to test is DiR
> > >(instead of DiO).
> > >
> > >Any more ideas out there? Plant, yeast, and fungi cells are good, but
> > >are there any more animal cell sources?
> > >
> > >Thanks for all replies so far.
> > >
> > >John Oreopoulos
> > >
> > >
> > >
> > >On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:
> > >
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >> *****
> > >>
> > >> Hi John
> > >> The use of onion cells for live cell confocal demonstrations
> > >>always works well.
> > >> Link
> > >>
> > >>
> > http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=on
> > ion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhC
> > Nafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=resul
> > t&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20con
> > focal&f=false
> > >>
> > >> Baggi Somasundaram
> > >> BioImaging Solutions
> > >> [hidden email]
> > >>
> > >> -----Original Message-----
> > >> From: Confocal Microscopy List
> > >>[mailto:[hidden email]] On Behalf Of John
> > >>Oreopoulos
> > >> Sent: Thursday, October 14, 2010 7:04 AM
> > >> To: [hidden email]
> > >> Subject: Cheap or free sources of cells
> > >>
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >> *****
> > >>
> > >> Dear confocal listserver,
> > >>
> > >> I'm looking for cheap or free sources of cells that can be used
> > >>for testing various fluorescent stains and/or microscopy
> > >>instrumentation. I've had good success in the past with blood cells
> > >>pricked from my finger with a finger lance and cheek cells scraped
> > >>from my mouth. I'm wondering if any of you out there know of any
> > >>other sources of cells for this same kind of purpose. Maybe urine
> > >>samples? Anything from nature/outdoors (besides pond water)?
> > >>Anything from the supermarket?
> > >>
> > >> Looking forward to all your creative responses!
> > >>
> > >> John Oreopoulos
> >
> > --
> > Philip Oshel
> > Microscopy Facility Supervisor
> > Biology Department
> > 024C Brooks Hall
> > Central Michigan University
> > Mt. Pleasant, MI 48859
> > (989) 774-3576
> >
>
>
>
> --
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email]
> URL: http://astro.temple.edu/~jbs <http://astro.temple.edu/%7Ejbs>
>