Adherence of satellite cells to glass

[Melia Paizi]

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Hi.

We need advice on how to improve adherence of satellite cells in culture to
optical glass.
We have tried matrigel, matrigel+polylysin, gelatin.

Thank you!


​​Melia Paizi, PhD

Bioimaging Center

​​Biomedical Core Facility

The Bruce Rappaport Faculty of Medicine

Technion - Israel Institute of Technology

POB 9649 MS 9, Bat-Galim, Haifa 3109601
<https://www.google.co.il/maps/dir//Medical+School,+Haifa/@32.83426,34.983802,17z/data=!4m13!1m4!3m3!1s0x151dbbd0803422e7:0x4bc667f2ea55e771!2sMedical+School!3b1!4m7!1m0!1m5!1m1!1s0x151dbbd0803422e7:0x4bc667f2ea55e771!2m2!1d34.983802!2d32.83426?hl=en>,
Israel

Tel. [phone] <[phone]>Fax. [phone] <[phone]>

[hidden email]

bcf.technion.ac.il

[Chris Guerin-2]

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Hi Melia:

        We have had good results with Cell Tak (http://catalog2.corning.com/LifeSciences/en-CN/Shopping/ProductDetails.aspx?categoryname=&productid=354240%28Lifesciences%29  no commercial interests, just satisfied users). So far it sticks every cell type we have tried. One caveat though is that in inhibiting movement you might also be changing the physiology.

Best wishes,

Chris Guérin
Manager, VIB Bio Imaging Core Ghent
       

[Maria Y. Boulina]

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Hi Melia,

I was just going to post the same:
http://csmedia2.corning.com/LifeSciences/media/pdf/SPC-354240.pdf
Cell-tak really works.
Regards,
Marcia Bulina

2016-05-18 9:10 GMT-04:00 Chris Guerin <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Melia:
>
>         We have had good results with Cell Tak (
> http://catalog2.corning.com/LifeSciences/en-CN/Shopping/ProductDetails.aspx?categoryname=&productid=354240%28Lifesciences%29
> no commercial interests, just satisfied users). So far it sticks every cell
> type we have tried. One caveat though is that in inhibiting movement you
> might also be changing the physiology.
>
> Best wishes,
>
> Chris Guérin
> Manager, VIB Bio Imaging Core Ghent
>

[Smith, Benjamin E.]

There are two ways to try matrigel, either as a slab or as a thin film.  In my experience, the thin-film worked very well for many different cell types.  Basically, you dilute the matrigel 1:10 and apply a thin layer to the cover slip and then allow it to completely air dry.  If the growth factors in matrigel prove to be altering the cell behavior, collagen is another option.

-Ben Smith

________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Melia Paizi <[hidden email]>
Sent: Wednesday, May 18, 2016 5:57:21 AM
To: [hidden email]
Subject: Adherence of satellite cells to glass

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Hi.

We need advice on how to improve adherence of satellite cells in culture to
optical glass.
We have tried matrigel, matrigel+polylysin, gelatin.

Thank you!


​​Melia Paizi, PhD

Bioimaging Center

​​Biomedical Core Facility

The Bruce Rappaport Faculty of Medicine

Technion - Israel Institute of Technology

POB 9649 MS 9, Bat-Galim, Haifa 3109601
<https://www.google.co.il/maps/dir//Medical+School,+Haifa/@32.83426,34.983802,17z/data=!4m13!1m4!3m3!1s0x151dbbd0803422e7:0x4bc667f2ea55e771!2sMedical+School!3b1!4m7!1m0!1m5!1m1!1s0x151dbbd0803422e7:0x4bc667f2ea55e771!2m2!1d34.983802!2d32.83426?hl=en>,
Israel

Tel. [phone] <[phone]>Fax. [phone] <[phone]>

[hidden email]

bcf.technion.ac.il

[daj1u06]

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Hi Melia,

we have had good success sticking post-culture, fixed mast cells to glass by
pretreating the glass with alcian blue.

The idea came from:
http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291097-0320%2820000401%2939:4%3C295::AID-CYTO7%3E3.0.CO;2-X/full

We do this to avoid flattening cells by cytospinning, especially where we
are attempting uptake studies, where imaging the cells in the round makes it
much easier to assess whether a fed probe is on or in.

I don't know if you could do this under sterile conditions for long term
live imaging though, as, from memory, if you try to filter sterilise alcian
blue solutions, you end up with a colourless filtrate and a blocked filter!

DAJ

David A Johnston,
Biomedical Imaging Unit, Faculty of Medicine, University of Southampton,
Mailpoint 12, Level B, Lab and Path Block,
Southampton General Hospital, Tremona Road, Southampton SO16 6YD