AiryScan vs. "superresolution" SHG?

[Mar Baha]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear colleagues,

have any of you tried the AiryScan (Zeiss) system together with a
multiphoton platform?
If yes, do you think is it possible to achieve true "SHG super resolution"?

Thanks in advance for your comments/opinion.
Best,
Mar

[Periasamy, Ammasi (ap3t)-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Yes, we collected 2photon Airyscan images in the fly brain and mouse embryo, the S/N is 2-3 times better than the conventional 2photon. The lateral resolution is also improved about 1.5 times in the fly brain at about 100 micron depth compared to confocal.
I would expect you should get better lateral resolution for SHG and THG imaging.
Hope this helps.
Ammasi

Dr. Ammasi Periasamy
Professor & Center Director,
WM Keck Center for Cellular Imaging,
Department of Biology, Univeristy of Virginia,
90 Geldard Dr., Charlottesville, VA 22904, USA.

http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]

FRET/FLIM Workshop-March 11-15, 2019: http://www.kcci.virginia.edu/workshop 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Mar Baha
Sent: Monday, May 21, 2018 7:05 PM
To: [hidden email]
Subject: AiryScan vs. "superresolution" SHG?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear colleagues,

have any of you tried the AiryScan (Zeiss) system together with a multiphoton platform?
If yes, do you think is it possible to achieve true "SHG super resolution"?

Thanks in advance for your comments/opinion.
Best,
Mar

[Roland Nitschke]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

We presented a talk at the FOM 2018:
http://www.focusonmicroscopy.org/2018/PDF/1096_Kleppe.pdf, that showed
strong improvement in image resolution and noise by using airy scan
detection for 2P fluorescence imaging down to a depth of 800 µm. We did
not use thicker samples for our tests. I expect it should be the same
for SHG imaging.

Best regards

Roland Nitschke


***********************************************************
*********************************************************** Dr. Roland
Nitschke Albert-Ludwigs University Freiburg Life Imaging Center (LIC) in
ZBSA Microscopy and Image Analysis Platform (MIAP) Habsburgerstr.49
79104 Freiburg Germany e-mail: [hidden email]
<mailto:[hidden email]> phone: [phone] fax:
[phone] web LIC:
https://miap.eu/miap-units/life-imaging-center-lic/ web MIAP:
https://miap.eu <https://miap.eu/>


Am 22.05.2018 um 01:30 schrieb Periasamy, Ammasi (ap3t):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Yes, we collected 2photon Airyscan images in the fly brain and mouse embryo, the S/N is 2-3 times better than the conventional 2photon. The lateral resolution is also improved about 1.5 times in the fly brain at about 100 micron depth compared to confocal.
> I would expect you should get better lateral resolution for SHG and THG imaging.
> Hope this helps.
> Ammasi
>
> Dr. Ammasi Periasamy
> Professor & Center Director,
> WM Keck Center for Cellular Imaging,
> Department of Biology, Univeristy of Virginia,
> 90 Geldard Dr., Charlottesville, VA 22904, USA.
>
> http://www.kcci.virginia.edu/people/profile/ap3t
> Phone: [phone] or 982-4869
> Fax: [phone]
> E-mail: [hidden email]
>
> FRET/FLIM Workshop-March 11-15, 2019: http://www.kcci.virginia.edu/workshop
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Mar Baha
> Sent: Monday, May 21, 2018 7:05 PM
> To: [hidden email]
> Subject: AiryScan vs. "superresolution" SHG?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear colleagues,
>
> have any of you tried the AiryScan (Zeiss) system together with a multiphoton platform?
> If yes, do you think is it possible to achieve true "SHG super resolution"?
>
> Thanks in advance for your comments/opinion.
> Best,
> Mar