Alexa Fluor 568 or 594 for 561nm laser?

[Adrian Smith-6]

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Hi all,

I was wondering if anyone has compared Alexa Fluor 568 to Alexa Fluor  
594 when excited by a 561nm laser?

Based on the spectral viewer the 568 will obviously be excited better  
but I've never used it so I don't know how it compares with regards to  
quantum efficiency and photostability etc?

Any other fluorochromes that people have found work well with that  
laser?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

[Kevin Braeckmans]

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Hi,

No quantitative information, but we have some users that are successfully
using the 561nm laser for excitation of Alexa 594 on our Nikon C1si. Other
dyes that we frequently use with this laser are Cy3, Rhodamine-B and TRITC.

Kind regards,

Kevin

> -----Oorspronkelijk bericht-----
> Van: Confocal Microscopy List [mailto:[hidden email]]
> Namens Adrian Smith
> Verzonden: vrijdag 12 september 2008 6:30
> Aan: [hidden email]
> Onderwerp: Alexa Fluor 568 or 594 for 561nm laser?
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
>
> I was wondering if anyone has compared Alexa Fluor 568 to Alexa Fluor
> 594 when excited by a 561nm laser?
>
> Based on the spectral viewer the 568 will obviously be excited better
> but I've never used it so I don't know how it compares with regards to
> quantum efficiency and photostability etc?
>
> Any other fluorochromes that people have found work well with that
> laser?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

[Michael Weber-4]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Adrian,

both dyes can be excited with the 561nm laser. Alexa 568 is often used
here in our facility - without bigger problems. If you are looking for a
dye especially for this laser, the 568 gets my recommendation. 594 also
works, but cannot be used in combination with something excited with
633/640 (Alexa 633/647, Draq5 etc.).

Michael

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
>
> I was wondering if anyone has compared Alexa Fluor 568 to Alexa Fluor
> 594 when excited by a 561nm laser?
>
> Based on the spectral viewer the 568 will obviously be excited better
> but I've never used it so I don't know how it compares with regards to
> quantum efficiency and photostability etc?
>
> Any other fluorochromes that people have found work well with that
> laser?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia