Amount of light discarded by a 1AU pinhole?

[Kai Schleicher]

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Hi,

Does anyone have a reference to clarify how much light in % is rejected
by a confocal pinhole at 1 AU compared to a widefield microscope?

Thanks and cheers,
Kai

--

Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel |
Phone: [phone] | [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch

[Craig Brideau]

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This would be strongly dependent on how much your sample scatters. An ideal
airy diffraction pattern has about 4/5 of the power in the main airy disk,
the rest being in the rings. In the real world though scattering will will
drastically decrease the light that gets through the pinhole.
https://spie.org/publications/fg01_p88_airy_disk

Craig

On Wed, Jun 15, 2016 at 2:44 PM, Kai Schleicher <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> Does anyone have a reference to clarify how much light in % is rejected by
> a confocal pinhole at 1 AU compared to a widefield microscope?
>
> Thanks and cheers,
> Kai
>
> --
>
> Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy |
> Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel |
> Phone: [phone] | [hidden email] |
> www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch
>

[Reece, Jeff (NIH/NIDDK) [E]]

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Wikipedia on Airy Disk also gives a reference for this, at the end of the "Mathematical details" section.

Cheers,
Jeff  

-----Original Message-----
From: Craig Brideau [mailto:[hidden email]]
Sent: Wednesday, June 15, 2016 5:07 PM
To: [hidden email]
Subject: Re: Amount of light discarded by a 1AU pinhole?

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This would be strongly dependent on how much your sample scatters. An ideal airy diffraction pattern has about 4/5 of the power in the main airy disk, the rest being in the rings. In the real world though scattering will will drastically decrease the light that gets through the pinhole.
https://spie.org/publications/fg01_p88_airy_disk

Craig

On Wed, Jun 15, 2016 at 2:44 PM, Kai Schleicher <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> Does anyone have a reference to clarify how much light in % is
> rejected by a confocal pinhole at 1 AU compared to a widefield microscope?
>
> Thanks and cheers,
> Kai
>
> --
>
> Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy
> | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056
> Basel |
> Phone: [phone] | [hidden email] |
> www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch
>

[Zdenek Svindrych-2]

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Hi Kai,
the confocal pinhole should block all the out-of-focus light. So if your
sample is 3D (from the microscope's point of view, that is, much thicker
than the z-resolution of the microscope), which is most of the time, the
pinhole will reject *most* of the light (99% in a thick densely labeled
specimen), when compared to widefield...

The Airy disc figures (> 80% transmission) are only meaningful for thin
samples (thin layer, subresolution beads, etc), if you want to compare to
widefield.
Best, zdenek



---------- Původní zpráva ----------
Od: Kai Schleicher <[hidden email]>
Komu: [hidden email]
Datum: 15. 6. 2016 16:54:40
Předmět: Amount of light discarded by a 1AU pinhole?

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*****

Hi,

Does anyone have a reference to clarify how much light in % is rejected
by a confocal pinhole at 1 AU compared to a widefield microscope?

Thanks and cheers,
Kai

--

Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy |
Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel |
Phone: [phone] | [hidden email] | www.biozentrum.unibas.
ch | www.microscopynetwork.unibas.ch"

[Guy Cox-2]

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We had a discussion on this topic just a few weeks ago - have a look down the list.

                                                        Guy

Guy Cox, Honorary Associate Professor
School of Medical Sciences

Australian Centre for Microscopy and Microanalysis,
Madsen, F09, University of Sydney, NSW 2006

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kai Schleicher
Sent: Thursday, 16 June 2016 6:44 AM
To: [hidden email]
Subject: Amount of light discarded by a 1AU pinhole?

*****
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*****

Hi,

Does anyone have a reference to clarify how much light in % is rejected by a confocal pinhole at 1 AU compared to a widefield microscope?

Thanks and cheers,
Kai

--

Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel |
Phone: [phone] | [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch