Any experience to share with confocal high content imaging hardware (W1, Dragonfly, Crest) and software?

[Emmanuel Levy]

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Dear All,

We currently have a confocal (W1) and are looking to buy a second confocal
with the goal to routinely image multiple 384 well plates and integrate the
system with a robotic loading arm.

I was curious if anyone has experience with high-content imaging with
software from different vendors, e.g., Olympus / Nikon / Zeiss / Leica /
Visitron / Metamorph / Andor? As well as hardware? e.g., W1, Dragonfly or
Crest?

Additionally, we're planning to use an automatic dispenser (water/silicon
or oil) to mount onto an objective, if anyone has
experience/recommendations with this it'd be great to hear about it.

Any feedback would be greatly appreciated,

Best wishes,

Emmanuel

[Sylvie Le Guyader]

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Dear Emmanuel

I love Nis-Elements and the crazy flexibility that the Jobs module offers. It is also very easy to use. We usually build the first pipeline for our users and they understand very fast how to modify it themselves.

We have an oil dispenser that we purchased ages ago from Emblem, the commercial wing of EMBL. We also have a home made water dispenser that works a charm and cost nothing to build. I can send you the specs if you want. It is based on an old paper.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

On 4 Jun 2019 23:55, Emmanuel Levy <[hidden email]> wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

We currently have a confocal (W1) and are looking to buy a second confocal
with the goal to routinely image multiple 384 well plates and integrate the
system with a robotic loading arm.

I was curious if anyone has experience with high-content imaging with
software from different vendors, e.g., Olympus / Nikon / Zeiss / Leica /
Visitron / Metamorph / Andor? As well as hardware? e.g., W1, Dragonfly or
Crest?

Additionally, we're planning to use an automatic dispenser (water/silicon
or oil) to mount onto an objective, if anyone has
experience/recommendations with this it'd be great to hear about it.

Any feedback would be greatly appreciated,

Best wishes,

Emmanuel




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[Emmanuel Levy]

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Dear Sylvie,

Thanks for your input. I'd be happy to hear more about the water dispenser.
I couldn't find the details of the oil dispenser on the EMBL-EM website. If
you have a reference it could maybe help.

Thank you,
Best wishes,

Emmanuel


On Wed, 5 Jun 2019 at 01:07, Sylvie Le Guyader <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Emmanuel
>
> I love Nis-Elements and the crazy flexibility that the Jobs module offers.
> It is also very easy to use. We usually build the first pipeline for our
> users and they understand very fast how to modify it themselves.
>
> We have an oil dispenser that we purchased ages ago from Emblem, the
> commercial wing of EMBL. We also have a home made water dispenser that
> works a charm and cost nothing to build. I can send you the specs if you
> want. It is based on an old paper.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
> On 4 Jun 2019 23:55, Emmanuel Levy <[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> We currently have a confocal (W1) and are looking to buy a second confocal
> with the goal to routinely image multiple 384 well plates and integrate the
> system with a robotic loading arm.
>
> I was curious if anyone has experience with high-content imaging with
> software from different vendors, e.g., Olympus / Nikon / Zeiss / Leica /
> Visitron / Metamorph / Andor? As well as hardware? e.g., W1, Dragonfly or
> Crest?
>
> Additionally, we're planning to use an automatic dispenser (water/silicon
> or oil) to mount onto an objective, if anyone has
> experience/recommendations with this it'd be great to hear about it.
>
> Any feedback would be greatly appreciated,
>
> Best wishes,
>
> Emmanuel
>
>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI
> kommer att behandla dina personuppgifter. Här finns information om hur KI
> behandlar personuppgifter<
> https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing
> your personal data. You can read more about KI’s processing of personal
> data here<https://ki.se/en/staff/data-protection-policy>.
>