Anyone experienced with a CrEST spinning disk on a Nikon system?

classic Classic list List threaded Threaded
2 messages Options
Avi Jacob Avi Jacob
Reply | Threaded
Open this post in threaded view
|

Anyone experienced with a CrEST spinning disk on a Nikon system?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I was wondering whether anyone has experience with the above mentioned
system. I would greatly appreciate any thoughts (privately if preferred).
Specifically as to the robustness (will be in a core), image quality, how
it compared to other spinning disks and the stated ability to easily remove
the disk from the light-pathway.

Also, with what light source? I was offered a SpectraX.

Thanks,
Avi


--
Avi Jacob, Ph.D.
Head of Light Microscopy
The Mina & Everard Goodman Faculty of Life Sciences
Bar-Ilan University, Ramat-Gan 5290002, Israel
G. Esteban Fernandez G. Esteban Fernandez
Reply | Threaded
Open this post in threaded view
|

Re: Anyone experienced with a CrEST spinning disk on a Nikon system?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I have an earlier generation in my core, when it was branded BD CARVII, on
a Zeiss Axiovert200M with a Hamamatsu ORCA CCD camera.

Since it doesn't have microlenses on the pinholes and it uses a lamp, not
lasers,  the light throughput is horrible. I've only used it with extremely
bright specimens. The new designs are quite different; I would compare to
Yokogawa spinning disks.

It has been extremely reliable and robust.  I use it for the filters and
move the spinning disc out of the light path routinely and very easily
thorough software. It is at least 10 years old and never had a problem.

-Esteban
On Feb 25, 2016 12:00 PM, "Avi Jacob" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I was wondering whether anyone has experience with the above mentioned
> system. I would greatly appreciate any thoughts (privately if preferred).
> Specifically as to the robustness (will be in a core), image quality, how
> it compared to other spinning disks and the stated ability to easily remove
> the disk from the light-pathway.
>
> Also, with what light source? I was offered a SpectraX.
>
> Thanks,
> Avi
>
>
> --
> Avi Jacob, Ph.D.
> Head of Light Microscopy
> The Mina & Everard Goodman Faculty of Life Sciences
> Bar-Ilan University, Ramat-Gan 5290002, Israel
>