Asante Potassium Green
Locked
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone! I am Isabel Romero, a PhD student, and I am working in the Department of Pharmacology, Faculty of Pharmacy, University of Granada, Spain. I am trying to measure K+ changes inside of some intestinal cell lines. For that, I need to use a Potassium Indicator dye. People use PBFI, but it requires UV excitation and we don´t have it. An alternative could be the Asante Potassium Green 1 and 2. I am trying to read something about that, but I guess there is not any publication untill now. I am wondering if some of you have done this staining or have some suggestion. Thank you very much! Best regards, Isabelius. Isabel Romero Calvo, PhD student. Department of Pharmacology, Faculty of Pharmacy, University of Granada. Spain. 0034 958243888 [hidden email] |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Isabel - we have worked with these dyes. Teflabs recently introduced APG3 and 4 with better selectivity for K. Of these, APG4 seems to have somewhat lower affinity (which is good). We just published a paper in Apoptosis (Kasim et al) available online where we show the expected decrease of K+ in apoptotic cells (that picture is in the Supplement). We have also done some work with APG using FACS. In short, the dye works but quantification may be a little tricky. Please contact me off list for more info. Best Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Isabel Romero Calvo [[hidden email]] Sent: Thursday, January 24, 2013 6:23 AM To: [hidden email] Subject: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone! I am Isabel Romero, a PhD student, and I am working in the Department of Pharmacology, Faculty of Pharmacy, University of Granada, Spain. I am trying to measure K+ changes inside of some intestinal cell lines. For that, I need to use a Potassium Indicator dye. People use PBFI, but it requires UV excitation and we don´t have it. An alternative could be the Asante Potassium Green 1 and 2. I am trying to read something about that, but I guess there is not any publication untill now. I am wondering if some of you have done this staining or have some suggestion. Thank you very much! Best regards, Isabelius. Isabel Romero Calvo, PhD student. Department of Pharmacology, Faculty of Pharmacy, University of Granada. Spain. 0034 958243888 [hidden email] |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike, How do APG3 and APG4 compare to PBFI, in your experience, in terms of signal change, sensitivity, and usage? Jason -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Friday, January 25, 2013 3:21 AM To: [hidden email] Subject: Re: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Isabel - we have worked with these dyes. Teflabs recently introduced APG3 and 4 with better selectivity for K. Of these, APG4 seems to have somewhat lower affinity (which is good). We just published a paper in Apoptosis (Kasim et al) available online where we show the expected decrease of K+ in apoptotic cells (that picture is in the Supplement). We have also done some work with APG using FACS. In short, the dye works but quantification may be a little tricky. Please contact me off list for more info. Best Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Isabel Romero Calvo [[hidden email]] Sent: Thursday, January 24, 2013 6:23 AM To: [hidden email] Subject: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone! I am Isabel Romero, a PhD student, and I am working in the Department of Pharmacology, Faculty of Pharmacy, University of Granada, Spain. I am trying to measure K+ changes inside of some intestinal cell lines. For that, I need to use a Potassium Indicator dye. People use PBFI, but it requires UV excitation and we don´t have it. An alternative could be the Asante Potassium Green 1 and 2. I am trying to read something about that, but I guess there is not any publication untill now. I am wondering if some of you have done this staining or have some suggestion. Thank you very much! Best regards, Isabelius. Isabel Romero Calvo, PhD student. Department of Pharmacology, Faculty of Pharmacy, University of Granada. Spain. 0034 958243888 [hidden email] |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hope to test it soon. Mike -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kilgore, Jason Sent: Tuesday, January 29, 2013 12:32 PM To: [hidden email] Subject: Re: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike, How do APG3 and APG4 compare to PBFI, in your experience, in terms of signal change, sensitivity, and usage? Jason -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Friday, January 25, 2013 3:21 AM To: [hidden email] Subject: Re: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Isabel - we have worked with these dyes. Teflabs recently introduced APG3 and 4 with better selectivity for K. Of these, APG4 seems to have somewhat lower affinity (which is good). We just published a paper in Apoptosis (Kasim et al) available online where we show the expected decrease of K+ in apoptotic cells (that picture is in the Supplement). We have also done some work with APG using FACS. In short, the dye works but quantification may be a little tricky. Please contact me off list for more info. Best Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Isabel Romero Calvo [[hidden email]] Sent: Thursday, January 24, 2013 6:23 AM To: [hidden email] Subject: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone! I am Isabel Romero, a PhD student, and I am working in the Department of Pharmacology, Faculty of Pharmacy, University of Granada, Spain. I am trying to measure K+ changes inside of some intestinal cell lines. For that, I need to use a Potassium Indicator dye. People use PBFI, but it requires UV excitation and we don´t have it. An alternative could be the Asante Potassium Green 1 and 2. I am trying to read something about that, but I guess there is not any publication untill now. I am wondering if some of you have done this staining or have some suggestion. Thank you very much! Best regards, Isabelius. Isabel Romero Calvo, PhD student. Department of Pharmacology, Faculty of Pharmacy, University of Granada. Spain. 0034 958243888 [hidden email] |
|
|
In reply to this post by Kilgore, Jason-2
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Don't know yet, hope to test it soon. APG are supposed to be much more specific for K. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kilgore, Jason Sent: Tuesday, January 29, 2013 12:32 PM To: [hidden email] Subject: Re: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Mike, How do APG3 and APG4 compare to PBFI, in your experience, in terms of signal change, sensitivity, and usage? Jason -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Friday, January 25, 2013 3:21 AM To: [hidden email] Subject: Re: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Isabel - we have worked with these dyes. Teflabs recently introduced APG3 and 4 with better selectivity for K. Of these, APG4 seems to have somewhat lower affinity (which is good). We just published a paper in Apoptosis (Kasim et al) available online where we show the expected decrease of K+ in apoptotic cells (that picture is in the Supplement). We have also done some work with APG using FACS. In short, the dye works but quantification may be a little tricky. Please contact me off list for more info. Best Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Isabel Romero Calvo [[hidden email]] Sent: Thursday, January 24, 2013 6:23 AM To: [hidden email] Subject: Asante Potassium Green ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone! I am Isabel Romero, a PhD student, and I am working in the Department of Pharmacology, Faculty of Pharmacy, University of Granada, Spain. I am trying to measure K+ changes inside of some intestinal cell lines. For that, I need to use a Potassium Indicator dye. People use PBFI, but it requires UV excitation and we don´t have it. An alternative could be the Asante Potassium Green 1 and 2. I am trying to read something about that, but I guess there is not any publication untill now. I am wondering if some of you have done this staining or have some suggestion. Thank you very much! Best regards, Isabelius. Isabel Romero Calvo, PhD student. Department of Pharmacology, Faculty of Pharmacy, University of Granada. Spain. 0034 958243888 [hidden email] |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |