Astigmatism

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Sarah Kefayati Sarah Kefayati
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Astigmatism

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello all!
 
Could you please help me with the best way to measure the astigmatism without any special optics?
 
thanks
Sarah
Paul Herzmark Paul Herzmark
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Re: Astigmatism

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Sarah,

You can determine your astigmatism by measuring a Point Spread Function.
Scatter beads around the field of view to see the PSF changes as a function of X and Y position.

No special optics needed.

Paul
-
Paul Herzmark
Specialist
[hidden email]

Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA 94720-3200
(510) 643-9603
(510) 643-9500 fax

On Thu, Jan 31, 2008 at 5:17 PM, Sarah Kefayati <[hidden email]> wrote:
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello all!
 
Could you please help me with the best way to measure the astigmatism without any special optics?
 
thanks
Sarah



-
Jennifer Waters Jennifer Waters
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Re: Astigmatism

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Check out:

Hiraoka, Y., Sedat, J. W., and Agard, D. A. (1990). Determination of three-dimensional imaging properties of a light microscope system. Biophysical J 57, 325-333.

Jennifer


On Feb 1, 2008 11:06 AM, Paul Herzmark <[hidden email]> wrote:
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Sarah,

You can determine your astigmatism by measuring a Point Spread Function.
Scatter beads around the field of view to see the PSF changes as a function of X and Y position.

No special optics needed.

Paul
-
Paul Herzmark
Specialist
[hidden email]

Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA 94720-3200
(510) 643-9603
(510) 643-9500 fax


On Thu, Jan 31, 2008 at 5:17 PM, Sarah Kefayati <[hidden email]> wrote:
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello all!
 
Could you please help me with the best way to measure the astigmatism without any special optics?
 
thanks
Sarah



-



--
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School
Ammasi Periasamy Ammasi Periasamy
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FLIM Theory

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Fernando Avila-Rencoret
Sent: Friday, May 02, 2008 11:06 AM
To: [hidden email]
Subject: FLIM theory

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I was wondering if somebody would help me to get some good "introductory"
article or tutorial (URLs are accepted!) for FLIM (Fluorescence lifetime
imaging microscopy).
----------
Hello

There are number of FLIM articles, book chapters and web sites are
available, if you go to Google and type "FLIM microscopy". You can also get
some chapters on FLIM theory and applications from my books
(1) Methods in Cellular Imaging (Oxford, 2001)
(2) Molecular Imaging: FRET Microscopy and Spectroscopy (Oxford, 2005). Now
this book is available in the Elsevier website. Of course you can get it
from Amazon.com Also, look at Joseph Lakowicz book on Principles of
Fluorescence Spectroscopy book.

Importantly, we do provide hands on training on FLIM-FRET imaging with live
specimens and FLIM and FRET theory and data analysis in our annual workshop
on FRET Microscopy during March.
http://www.kcci.virginia.edu/workshop/index.php

Robert Clegg and myself is working on a FLIM book which we expect to be
published by Taylor & Francis - CRC Press group during March 2009. This book
will pedagogically explain the basics of FLIM, physical principles behind
the technique or methodology, various data analysis approaches and
interpretation of the data, and connect them especially useful for biology
and medicine.

Best wishes,
Ammasi



Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI) Professor of Biology and
Biomedical Engineering Biology, Gilmer Hall (064), McCormick Rd University
of Virginia Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210;
Email:[hidden email] http//:www.kcci.virginia.edu
************************
Workshop on FRET Microscopy, March 3-7, 2009
http://www.kcci.virginia.edu/workshop/workshop2009/index.php
  *************************