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Hello all!
Could you please help me with the best way to measure the astigmatism without any special optics?
thanks
Sarah
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Sarah,
You can determine your astigmatism by measuring a Point Spread Function. Scatter beads around the field of view to see the PSF changes as a function of X and Y position. No special optics needed. Paul - Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax On Thu, Jan 31, 2008 at 5:17 PM, Sarah Kefayati <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal - |
Search the CONFOCAL archive at
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Check out: Hiraoka, Y., Sedat, J. W., and Agard, D. A. (1990). Determination of three-dimensional imaging properties of a light microscope system. Biophysical J 57, 325-333. JenniferOn Feb 1, 2008 11:06 AM, Paul Herzmark <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Sarah, -- Jennifer Waters, Ph.D. Director, Nikon Imaging Center at Harvard Medical School |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Fernando Avila-Rencoret Sent: Friday, May 02, 2008 11:06 AM To: [hidden email] Subject: FLIM theory Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I was wondering if somebody would help me to get some good "introductory" article or tutorial (URLs are accepted!) for FLIM (Fluorescence lifetime imaging microscopy). ---------- Hello There are number of FLIM articles, book chapters and web sites are available, if you go to Google and type "FLIM microscopy". You can also get some chapters on FLIM theory and applications from my books (1) Methods in Cellular Imaging (Oxford, 2001) (2) Molecular Imaging: FRET Microscopy and Spectroscopy (Oxford, 2005). Now this book is available in the Elsevier website. Of course you can get it from Amazon.com Also, look at Joseph Lakowicz book on Principles of Fluorescence Spectroscopy book. Importantly, we do provide hands on training on FLIM-FRET imaging with live specimens and FLIM and FRET theory and data analysis in our annual workshop on FRET Microscopy during March. http://www.kcci.virginia.edu/workshop/index.php Robert Clegg and myself is working on a FLIM book which we expect to be published by Taylor & Francis - CRC Press group during March 2009. This book will pedagogically explain the basics of FLIM, physical principles behind the technique or methodology, various data analysis approaches and interpretation of the data, and connect them especially useful for biology and medicine. Best wishes, Ammasi Ammasi Periasamy, Ph.D. Director, Keck Center for Cellular Imaging (KCCI) Professor of Biology and Biomedical Engineering Biology, Gilmer Hall (064), McCormick Rd University of Virginia Charlottesville, VA 22904 Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http//:www.kcci.virginia.edu ************************ Workshop on FRET Microscopy, March 3-7, 2009 http://www.kcci.virginia.edu/workshop/workshop2009/index.php ************************* |
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