Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalDear all,
I want to conjugate a red fluorophore to a monoclonal rat antibody. The
imaging setup is optimized for eGFP/mCherry imaging, so I'm looking for
good fluorophores, either green or red. How does the Atto dyes from
Atto-Tec compare to Molecular Porbes' Alexa dyes (brightness, bleaching,
live-cell imaging) ? Specifically, I'd be interested in inputs for
Atto488 vs Alexa488 and Atto590 vs Alexa594.
Is it simple to couple fluorophores (I've never done it) ? Can I just by
the NHS ester and a column or is it better to buy a (rather expensive)
labeling kit from Invitrogen (Alexa dyes) or Jena (Atto dyes )?
Thank you for your informations and advices,
--
Christophe Leterrier
Postdoc
INSERM UMR641 Neurobiology of ionic channels
IFR Jean Roche
<
http://ifrjr.nord.univ-mrs.fr/-Institut-Jean-Roche-?lang=en> -
Mediterranee University
Marseille, France
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