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Dear Sarah,
If all bead images are almost same size, you could do interactive alignment of bead images with imagej plugin turboreg (
http://bigwww.epfl.ch/thevenaz/turboreg/). It is based on calculating affine transform based on control points that you can interactively select.
I haven't used it but my labmate found it useful in aligning the images that were taken with different condenser positions. Her problem was that the microscope shook too much when motorized condenser was rotated.
regards
shalin
On Sun, Jun 8, 2008 at 12:52 AM, Sarah Kefayati <
[hidden email]> wrote:
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Dear list,
I need to average over different images of the single beads which are acquired in the same depth but different locations.I don't have any software in order to do that by the image-processing techniques.
This is my approach:
I crop a single bead image.then I lower the size(to the half of the size) of the pixel by the zoom factor.next I plot the intensity vs. position graph and I assume that the peak of the graph represents the center of the bead.then I move the cropping box till I locate the highest intensity pixel to the pixel in the center of the box.next I zoom back again to the original size and save the image and by the option available in the software (fluview v. 5) I add the images together.I also use sixfold kalman averaging for each image.
I appreciate any comment on this approach in order to do that more accurately or any other suggestion.
Regards
Sarah
--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
mobile: +65-90694182
blog:
shalin.wordpress.com~~~~~~~~~~~~~~~~~~~~~~~~~~
Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website:
http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html
Liver Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore 169610
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