Axicon TIRF

[Jens]

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Dear List,

has anybody ever built a TIRF system using an Axicon zoom system to produce an annular ring of excitation at the BFP?

Thanks!

Jens

--------------------------------------------------------------
Jens B. Bosse Ph.D.
Enquist Lab
Department of Molecular Biology
and
Princeton Neuroscience Institute
Princeton University
301 Schultz Lab
Washington Rd
08544 Princeton, NJ, USA

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Web: http://molbio.princeton.edu/labs/enquist/

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[David Baddeley]

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Hi Jens,
you might want to get in touch with Derek Toomre at Yale - he has just such a system, and it is still in active use in his lab. I can't find the paper at present, but  it has since been licensced to GE - the patent is available here: Patent EP1941313A2 - An optical system for illumination of an evanescent field
cheers,David