BABB mounting media.

[Goodhouse, Joseph G.]

Could someone please send me a recipe and protocol for BABB mounting media.

Regards,

Joe Goodhouse
Confocal Core Lab Manager
Dept. of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio1.princeton.edu/facility/confocal/  

[Kurt Thorn]

BABB is just 1/3 benzyl alcohol and 2/3 benzyl benzoate. To mount
samples in it they must first be thoroughly dehydrated.  It clears very
well, but it can cause some changes in tissue morphology.  Methyl
salicylate is also worth trying.

Kurt

Goodhouse, Joseph G. wrote:

>
> Could someone please send me a recipe and protocol for BABB mounting
> media.
>
> Regards,
>
> Joe Goodhouse
> Confocal Core Lab Manager
> Dept. of Molecular Biology
> Princeton University
> 609-258-5432
>
> Visit us at _http://www.molbio1.princeton.edu/facility/confocal/_
> <http://www.molbio1.princeton.edu/facility/confocal/>  
>
>

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone [phone]
fax   [phone]

[Zucker.Robert]

Joe
   A review article has been published that has all the latest BABB
   clearing details that we used in this technique
   The article is available on request. Most of your questions have been
   answered in this publication.
   Bob

   1. Zucker, R.M.Technical note: Whole insects and Mammalian Embryo
   Imaging with   Confocal Microscopy: Morphology and Apoptosis.
   Cytometry 2006 69A: 1143-1152

Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Reproductive Toxicology Division
Telephone: [phone]   Fax: [phone]
e-mail: [hidden email]

Mail address: USEPA,ORD,NHEERL,RTD
Developmental Biology Branch ( MD 67)
Research Triangle Park, North Carolina, 27711

Shipping address:
2525 E.NC Highway 54
Durham, NC, 27713



                                                                       
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Could someone please send me a recipe and protocol for BABB mounting
media.


Regards,


Joe Goodhouse
Confocal Core Lab Manager
Dept. of Molecular Biology
Princeton University
609-258-5432


Visit us at http://www.molbio1.princeton.edu/facility/confocal/