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Hi,
Has anybody tried a Brilliant Violet 421 secondary antibody in a typical
expansion microscopy protocol? I would be grateful if you could share
your opinions. As you know, some fluorochromes fade substantially during
the expansion process.
Also, using widefield microscopy in the DAPI channel*, we see a lot of
background (especially nuclei**) in negative controls (no DAPI). Anybody
has observed something similar?
*DAPI channel is 387/11 ex; Semrock DA/FI/TR/Cy5 4 band dichroic, 440/40 em.
** primary neurons grown on poly-lysine coated 5 coverslips.
Javier
Fco. Javier Díez Guerra, PhD
Centro de Biología Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Universidad Autónoma de Madrid
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN
phone: +34 91 196 4612
e-mail:
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