Beads for rapid protein release during imaging

[Eric Olson]

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Dear List,

I am looking for beads that I can load with recombinant proteins, wash in
saline and then inject into a brain explant. The idea is to provide a
localized source of the protein that releases over the course of a few hours
(not days) - while I am imaging.

When I have injected the protein by itself, the protein becomes widely
distributed very rapidly (minutes) which is not good for these experiments.

Ideally, the beads would not be larger than 10 micrometers in diameter and
could be fluorescent.

Does anyone have any experience with this sort of application?

Thanks,

Eric

[George McNamara]

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Robert Langer, MIT is the scientist most associated with slow or fast
release matrices. See

http://web.mit.edu/langerlab/publications/2001.html
(may need other years)

He also cofounded    
http://www.modernatx.com/about?gclid=CM7_g6_7vrcCFUdk7AoddjIANg     -
you might be able to work with them on samples.

George

On 5/30/2013 12:44 PM, Eric Olson wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List,
>
> I am looking for beads that I can load with recombinant proteins, wash in
> saline and then inject into a brain explant. The idea is to provide a
> localized source of the protein that releases over the course of a few hours
> (not days) - while I am imaging.
>
> When I have injected the protein by itself, the protein becomes widely
> distributed very rapidly (minutes) which is not good for these experiments.
>
> Ideally, the beads would not be larger than 10 micrometers in diameter and
> could be fluorescent.
>
> Does anyone have any experience with this sort of application?
>
> Thanks,
>
> Eric
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054