Best red tag for co-localisation with GFP?

[Dolphin, Colin]

Simple question from a relative newbie - we are co-injecting mRNA encoding GFP and an oligo into C. elegans and would like to tag the oligo with a fluorophore so we can track its localisation, half-life, etc. Which red tag would be best in combination with GFP?

Colin

[Dries Vercauteren]

Dear Colin,

any far red dye (emission between 650 and 750) would be suitable. A good example that I use is Cy5.

Good luck!

Dries.

2009/6/25 Dolphin, Colin <[hidden email]>

Simple question from a relative newbie - we are co-injecting mRNA encoding GFP and an oligo into C. elegans and would like to tag the oligo with a fluorophore so we can track its localisation, half-life, etc. Which red tag would be best in combination with GFP?

Colin

--
Dries Vercauteren, PhD student
Master of Bioscience Engineering: Cell and Gene Biotechnology

Ghent Research Group on Nanomedicines
www.ugent.be/fw/en/research/biofys
Faculty of pharmaceutical sciences, Ghent University
Harelbekestraat 72, 9000 Ghent
Belgium

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