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Dear Christophe,
We did this with developing mouse eggs or blastula stage embryos.
Sigma has some plastic coverwells that can be sticked to glass.
"Grace Bio-Labs CoverWell? imaging chambers" they call it.
We sticked these chambers not to slides but to coverglass of the same
size. So we get a wet compartment that contained floating embryos.
Mounted in a home made holder we then looked at it by confocal
microscopy. The fixed and stained specimen were successfuly send from
US to Belgium. The other advantage is that you can see the other side
of the embryo if you invert your specimen. I wonder if these things
are also avaiable in glass but if yes, you can have optimal image qualit
This methods was used in our publications with Pravin Goud.
Bye,
Patrick
--
Dep. Moleculaire Biotechnologie
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Citeren Christophe Leterrier <
[hidden email]>:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear microscopists,
>
> I have fixed and stained cell cultures as coverlips in wells of a
> 12-well plate in aqueous medium. I want to send these coverslips
> accross the country for imaging by a collaborator. They will be imaged
> "wet" in PBS so mounting isn't an option.
>
> Did someone already do that kind of thing? What is the best way to
> achieve this?
>
> Thanks a lot for your help,
>
> Christophe
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