Bidirectional scanning on olympus FV1000

[Shalin Mehta]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list,

 During a demonstration today, I came to know that FV1000 doesn't allow imaging more than two channels during bidirectional scan. I don't appreciate why? and couldn't get clear explanation.
Thinking more about it, to me it seems that bidirectional scanning is not bad inherently. One should be able to know pixel locations and measure signal as accurately as in unidirectional scanning with flyback.

What is the instrument limitation that leads to noisy and 'streaky' data during bi-directional scanning?

thanks
shalin

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~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~
Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html

Liver Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore 169610
http://www.nccs.com.sg/researcher/02_04d.htm

[Craig Brideau]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal As I understand it, the 'streaky' is from the hang time as the galvo changes direction.  Apparently this is different under different circumstances somehow and has to be compensated for either with image registration software or a human manually shifting the alternate lines.
The two channel limitation may be due to a speed limit on the data acquisition hardware on the microscope.  Maybe it just can't shovel pixel data into the system fast enough?  Just guessing here though; talk to your sales rep.

Craig

On Fri, Jul 11, 2008 at 6:24 AM, Shalin Mehta <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list,

 During a demonstration today, I came to know that FV1000 doesn't allow imaging more than two channels during bidirectional scan. I don't appreciate why? and couldn't get clear explanation.
Thinking more about it, to me it seems that bidirectional scanning is not bad inherently. One should be able to know pixel locations and measure signal as accurately as in unidirectional scanning with flyback.

What is the instrument limitation that leads to noisy and 'streaky' data during bi-directional scanning?

thanks
shalin

--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~
Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html

Liver Cancer Functional Genomics Lab, #6-05
National Cancer Centre, Singapore 169610
http://www.nccs.com.sg/researcher/02_04d.htm