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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi all,
I'm wondering if anyone else has paid attention to the news that some
plasticware companies have pulled their products off the markets due
to the leaching of estrogenic compounds from bottles and such (Nalgene
did a couple of weeks ago).
I know some folks have very carefully looked into this issue in the
context of cell culture plasticware, and found that some plates do a
pretty good job of mimicking estrogen in growth assays using breast
cancer cells, presumably due to the use of Bisphenol A (or perhaps
other plasticizers).
We've been doing steroid receptor work for years using glass
coverslips, and more recently with glass bottom 96/384 well plates.
In these glass bottom plates, where there is an increased plastic wall-
to-glass-ratio in a small well (compared to a dish), we've seen
visible evidence of estrogenic activity (GFP-ER assays), and have had
to find replacement plates to avoid uninterpretable results.
I'm curious if anyone else has noticed any such phenomenon in their
assays, that might also be attributable to potential estrogenic
activity of the plastics being used.
Mike
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Michael A. Mancini, Ph.D.
Associate Professor
Department of Molecular and Cellular Biology
M803 DeBakey Building
Baylor College of Medicine
One Baylor Plaza
Houston, TX 77030
713.798.8952.office
713.798.3175.fax
713.408.0179.cell
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