Hi Marc,
In the presence of Prolong Gold (and for the most part in PBS) our
analysis shows for photostability MarinaBlue>AlexaFluor405>Pacific Blue.
PacBlue is brighter out of the gate, but does fade quicker. Thus
MarinaBlue is often the preferred choice for imaging, while PacBlue is
used in FLOW.
I can send bleach curves off line if you like, as we have done several
times in the past when this has been asked.
Regards,
Mike Ignatius
Molecular Probes/Life Tech (showing no grudges that USC beat the Oregon
Ducks last night.)
-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Marc Green
Sent: Thursday, March 05, 2009 8:01 PM
To:
[hidden email]
Subject: Blue-violet secondary
Dear list,
I am looking to match a secondary to my DAPI filter set.
I would appreciate anybodies experience/opinions on photostability and
brightness for Marina Blue, Alexa 350, or similar.
Thanks.
------------------
Marc D Green
[hidden email]
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