CARS imaging on a scanning confocal

[Csúcs Gábor-3]

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Dear All,

For a user project we would like to establish CARS imaging on one of our scanning confocals (more precisely on a multi-photon system). Unfortunately from the "big fours" (according my information) only Leica offers a commercial solution for CARS but they use a special laser (which for various reasons is not an option for us). Our idea would be to use a "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual wavelength option) as light source. Ideally we would install this modality on a Zeiss LSM multiphoton system. My question is hence twofold:
1) Is there anyone who has established CARS imaging on a Zeiss confocal?
2) Is there anyone who has used the above mentioned laser for realizing CARS?

I would very much appreciate any feedback, experience report.

Thanks           Gabor

[George McNamara]

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Hi Gabor,

Olympus also offers CARS commercially, using DeepSea laser (in the US
and Canada, anyway). Try to have both transmitted and epi detectors -
both have uses (and you could replce the standard condenser with another
objective lens, if desired).

http://www.olympus-lifescience.com/en/laser-scanning/fv1200mpe/femtocars/
The femtoCARS equipped FV1200MPE provides a label-free lipid imaging
capability based on molecular vibrations using the Coherent Anti-Stokes
Raman Scattering (CARS) microscopy method. In the Olympus design, the
CARS imaging mode can be combined with two photon excitation
fluorescence (TPEF) and second harmonic generation (SHG) imaging modes
as a multimodal femtosecond laser imaging station. CARS is one of a
number of intrinsic imaging modes requiring no extrinsic label. Because
it relies on molecular vibrations rather than traditional fluorescence,
there is no photobleaching.


      Summary

    * Simultaneous Lipid (CH2), TPEF, and SHG imaging. The lipid filter
      set is included and TPEF and SHG filter sets are optional.
    * Uses the standard Olympus DeepSee lasers which are specifically
      tuned to match Olympus optical requirements. No additional laser
      is required.
    * The output of the femtoCARS is pre-set to lipid detection.
    * Standard MPE detection system, up to two forward (transmitted) and
      four backward (epi) Non-Descanned Detection channels.
    * Standard MPE imaging (by-pass CARS add-on) and single photon
      imaging are available.
    * The optical delivery system including a laser-safe enclosure is
      provided and serviced by Olympus Scientific  Solutions Americas
    * The overall footprints for MPE with femtoCARS remain small, but
      different from regular MPE systems.
    * Upright platforms are standard. For inverted platform, please
      contact Olympus Scientific Solutions Americas
    * Acousto-optical modulation (AOM) standard for MPE and manual power
      adjustment operation for the Stokes' beam.
    * Dispersion compensation standard for MPE and for efficient Stokes'
      beam generation.


A couple of CAR reviews

http://www.jlr.org/content/51/11/3091.full
This review reported that CARS (or at least CRS) was first reported by
a  car company!:

The phenomenon of coherent Raman scattering was first reported by
scientists at the Ford Motor Co. in 1965 (28
<http://www.jlr.org/content/51/11/3091.full#ref-28>). Since the
introduction in the early 1970s (29
<http://www.jlr.org/content/51/11/3091.full#ref-29>), CARS spectroscopy
has been widely used as a spectroscopic tool (30
<http://www.jlr.org/content/51/11/3091.full#ref-30>). The first
application of CARS to imaging was demonstrated in 1982 with a
noncollinear beam geometry by scientists at the Naval Research
Laboratory (31 <http://www.jlr.org/content/51/11/3091.full#ref-31>).
Nearly two decades later, CARS microscopy was revisited with a collinear
beam configuration at the Pacific Northwest National Laboratory in 1999
(32 <http://www.jlr.org/content/51/11/3091.full#ref-32>). Since then,
the CARS renaissance has stimulated further development and applications
by researchers from broad scientific disciplines. Continuous
contributions from many research groups over the last decade have vastly
improved the design, stability, and sensitivity of CARS microscopy [for
reviews, see (18 <http://www.jlr.org/content/51/11/3091.full#ref-18>, 23
<http://www.jlr.org/content/51/11/3091.full#ref-23>--25
<http://www.jlr.org/content/51/11/3091.full#ref-25>)].


http://www.chem.uci.edu/~potma/AlbaJBO14.pdf
I liked their figure 6, but why did they just go hyperspectral? If you
go spectral, I suggest branding it super-duper-hyper-multi-spectral
coherent anti-Stokes Raman super-duper resolution nanoscopy.


best wishes,

George



On 6/23/2015 5:36 AM, Csúcs  Gábor wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> For a user project we would like to establish CARS imaging on one of our scanning confocals (more precisely on a multi-photon system). Unfortunately from the "big fours" (according my information) only Leica offers a commercial solution for CARS but they use a special laser (which for various reasons is not an option for us). Our idea would be to use a "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual wavelength option) as light source. Ideally we would install this modality on a Zeiss LSM multiphoton system. My question is hence twofold:
> 1) Is there anyone who has established CARS imaging on a Zeiss confocal?
> 2) Is there anyone who has used the above mentioned laser for realizing CARS?
>
> I would very much appreciate any feedback, experience report.
>
> Thanks           Gabor
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42

[WHEELER Ann]

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Hi Gabor

We do a fair bit of Cars at the IGMM. Can I ask you to send me a personal email and I can put you in touch with the people who are doing it. We use CARs on a multiphoton system and I'm sure can advise about appropriate lasers.

Best wishes

Ann


Dr Ann Wheeler
Advanced Imaging Resource, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU
E: [hidden email]
T: 0131 651 8665
W: http://www.igmm.ac.uk/imaging.htm




--
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: 23 June 2015 11:37
To: [hidden email]
Subject: CARS imaging on a scanning confocal

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Dear All,

For a user project we would like to establish CARS imaging on one of our scanning confocals (more precisely on a multi-photon system). Unfortunately from the "big fours" (according my information) only Leica offers a commercial solution for CARS but they use a special laser (which for various reasons is not an option for us). Our idea would be to use a "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual wavelength option) as light source. Ideally we would install this modality on a Zeiss LSM multiphoton system. My question is hence twofold:
1) Is there anyone who has established CARS imaging on a Zeiss confocal?
2) Is there anyone who has used the above mentioned laser for realizing CARS?

I would very much appreciate any feedback, experience report.

Thanks           Gabor

[Craig Brideau]

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You might want to check out the several CARS papers my colleague Kelvin
Poon and I have presented at Photonics West over the years. It details our
own CARS system which is almost completely built from scratch. I
constructed it using a Nikon C1 scan head, but the rest of the microscope
is all bulk parts. The 2013 papers in particular should be useful.

http://spie.org/profile/Craig.Brideau-82296
http://spie.org/profile/Kelvin.Poon-143210

Craig

On Tue, Jun 23, 2015 at 7:26 AM, WHEELER Ann <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Gabor
>
> We do a fair bit of Cars at the IGMM. Can I ask you to send me a personal
> email and I can put you in touch with the people who are doing it. We use
> CARs on a multiphoton system and I'm sure can advise about appropriate
> lasers.
>
> Best wishes
>
> Ann
>
>
> Dr Ann Wheeler
> Advanced Imaging Resource, Institute of Genetics and Molecular Medicine,
> University of Edinburgh, Edinburgh EH4 2XU
> E: [hidden email]
> T: 0131 651 8665
> W: http://www.igmm.ac.uk/imaging.htm
>
>
>
>
> --
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Csúcs Gábor
> Sent: 23 June 2015 11:37
> To: [hidden email]
> Subject: CARS imaging on a scanning confocal
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> For a user project we would like to establish CARS imaging on one of our
> scanning confocals (more precisely on a multi-photon system). Unfortunately
> from the "big fours" (according my information) only Leica offers a
> commercial solution for CARS but they use a special laser (which for
> various reasons is not an option for us). Our idea would be to use a
> "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual
> wavelength option) as light source. Ideally we would install this modality
> on a Zeiss LSM multiphoton system. My question is hence twofold:
> 1) Is there anyone who has established CARS imaging on a Zeiss confocal?
> 2) Is there anyone who has used the above mentioned laser for realizing
> CARS?
>
> I would very much appreciate any feedback, experience report.
>
> Thanks           Gabor
>