Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalKira, have you tried looking at thick sections of spleen or lymph node stained with CD8? You should have screaming contrast between T cell zones as a positive control.
I have used rat x mouse CD8 from Chemicon with good success.
For neurons, NeuN (Chemicon) and Nissl stains (Invitrogen) should be quite positive as well in brain.
GL
Melissa
Melissa A. González Edick
R&D, Cell Genesys Inc.
500 Forbes Blvd
South San Francisco, CA 94080
p(650) 266-3168
f (650) 266-3080
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Lathrop, Kira L
Sent: Tuesday, November 06, 2007 1:00 PM
To:
[hidden email]
Subject: CD8 & neuronal staining?
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi everyone,
We're working with an Olympus Fluoview confocal microscope imaging thick
sections (100 um) of the trigeminal ganglia (mouse) and are trying to
stain for CD8's as well as the neuronal marker. Is anyone doing similar
work or have you had success with a staining protocol?
Thanks in advance for any help
Tom Cherpes
Kira Lathrop
Kira Lathrop, MAMS
Sr. Research Specialist
University of Pittsburgh
Eye and Ear Institute
203 Lothrop Street Rm 1026
Pittsburgh, PA 15261
[hidden email]
412-647-3492
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