CO2 measurement

[Romin, Yevgeniy/Sloan Kettering Institute]

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Dear List

We are concerned with the accuarcy of CO2 readouts on our incubation systems.  We would like to have a tool that can measure not the CO2 concentration coming out of the unit, but right at the objective or even in the dish with a sample.  We tried different things, such as measuring the pH of the media and basic viability assays, but we would still like something more precise.  Our lab operations depratment brought down a probe that they use to measure CO2 levels in culture incubators, but the probe on that instrument is too big to measure inside the stage incubators on our systems.  If anybody here had experience with this and can recommend and instrument for purchasing, or any other input, it will be greatly appreciated.

Thanks very much to all of you in advance,

Yevgeniy Romin

 
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[jlribas]

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Dear Romin,
This could be interesting for you. No commercial interest.

https://www.harvardapparatus.com/hapdfs/HAI_DOCCAT_3/RM1_69.pdf

All the best

Juan Luis

El 30/01/2012 21:36, Yevgeniy Romin escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List
>
> We are concerned with the accuarcy of CO2 readouts on our incubation systems.  We would like to have a tool that can measure not the CO2 concentration coming out of the unit, but right at the objective or even in the dish with a sample.  We tried different things, such as measuring the pH of the media and basic viability assays, but we would still like something more precise.  Our lab operations depratment brought down a probe that they use to measure CO2 levels in culture incubators, but the probe on that instrument is too big to measure inside the stage incubators on our systems.  If anybody here had experience with this and can recommend and instrument for purchasing, or any other input, it will be greatly appreciated.
>
> Thanks very much to all of you in advance,
>
> Yevgeniy Romin
>
>
>       =====================================================================
>
>       Please note that this e-mail and any files transmitted from
>       Memorial Sloan-Kettering Cancer Center may be privileged, confidential,
>       and protected from disclosure under applicable law. If the reader of
>       this message is not the intended recipient, or an employee or agent
>       responsible for delivering this message to the intended recipient,
>       you are hereby notified that any reading, dissemination, distribution,
>       copying, or other use of this communication or any of its attachments
>       is strictly prohibited.  If you have received this communication in
>       error, please notify the sender immediately by replying to this message
>       and deleting this message, any attachments, and all copies and backups
>       from your computer.
>

--
Juan Luis Ribas
Servicio de Microscopía
Centro de Investigación, Tecnología e Innovación
Universidad de Sevilla
Av. Reina Mercedes 4b
41012 Sevilla

Tfno: 954559983