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COVID19: Instrument cleaning protocols

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Jacqueline Ross

Mar 15, 2020; 8:14pm

Re: COVID19: Instrument cleaning protocols

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Hi everyone,

This has been interesting reading. We currently have 8 confirmed and 3 probable cases in New Zealand. I know this is a very small number compared with many other countries and our sympathies are with you all. However, winter is coming and our government has now stepped up with stronger restrictions. Our university has banned all international travel and recalled all staff who are currently overseas.

In regard to our core facility, I intend to send an email to all microscope users reminding them that they must wash their hands thoroughly with soap (preferred option) or use hand sanitizer before leaving the lab. This is our normal practice.

However I will also be asking all users to also wash their hands before using the microscopes, i.e. after entering the main lab. Based on these discussions, I intend to also ask people to clean the eyepieces before and after using the microscope.

I am also considering removing all plastic/rubber eye cups from the eyepieces as an extra precaution. What do people think about that idea?

Like Michael, we have a "no gloves rule" apart from when people are working with live samples. They can wear gloves when handling their specimen but only when putting it onto and off the microscope
Gloves must be removed before touching any other components such as focus wheel, joy stick, etc.

For us, it's not practicable for us to clean microscopes between users and I wouldn't feel comfortable leaving anything like bleach around in case some of the more anxious users decided to spray it around.

We use Accel wipes (peroxide) for benches so I will probably wipe those myself each day and also all door handles. However if everyone observes good hand hygiene, the internal room handles should be low risk.

Kind regards,

Jacqui

Jacqueline Ross
Lead Technologist
Optical Microscopy & Image Analysis
Biomedical Imaging Research Unit (BIRU)
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Telephone: Ext 87438; DDI: +64 9 923 7438

Website: http://www.auckland.ac.nz/biru

________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Cammer, Michael [[hidden email]]
Sent: 15 March 2020 08:26
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

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I have read many, but not all of, the messages in this thread, so apologies if some redundancy.

The following response is a few tips for microscope decontamination for BSL-2 (and in some cases BSL-3), not specifically for the corona virus, but I believe these points apply and some of them are for equipment protection while also fulfilling the goal of microorganism or virus decontamination. They could be extended to decontamination of scopes and ancillary equipment as per users who may be carrying contagious illnesses. I am writing from the point of view of a core facility with scopes used by many people and in scattered locations with minimal or no supervision. These are more practical tips we have learned (the hard way) for operating as part of more thorough protocols.

Paper goods: We do not allow commercial tissues in the lab. We have had people with colds come to use the microscopes and bring their own box of tissues with them to wipe their noses while using our equipment. (Yes, this is true.) They will use these tissues on the equipment if they are within reach. Tissues are likely to scratch optics and leave dust everywhere. Paper goods need to be limited to lens tissue and wood fiber only Kimwipes.

70% EtOH and 10% bleach must be kept in bottles that look completely different. People will get them confused otherwise and bleach sprayed on optics is not good. A bottle with a white top and a bottle with a red top with Sharpie marker writing on the side are not different enough. We solve this problem by supplying the 70% EtOH in clear hand labeled bottles and the bleach in prepackaged Clorox bottles. (The clear bottles are marked with Sharpie and then clear packing tape over the writing to prevent the ink from being washed off because, like immersion oil somehow getting applied to every surface, EtOH will run down the side of the bottle. The Clorox bottles are also labeled in BIG letters, Microscopy, to reduce "borrowing" of them; when you need the bleach, you need it next to the scope, not in somebody else's lab.)

Using prepackaged bleach has another benefit. It is buffered so it remains active longer. The downside of this is that it may leave a fine power residue. However, this is preferential to having people mistaking bottles and spraying bleach on optics. The surfaces may be wiped down a second time with EtOH, water, or lens cleaner to remove the powder.

70% EtOH may damage plastics (other people have mentioned this). Beware overuse on Plexiglas boxes, plastic knobs, and painted surfaces. We have had environmental chambers on scopes develop networks of microcracks due to too much EtOH. If you want to decontaminate these surfaces, we recommend 10% bleach.

Other people have mentioned methanol and its toxicity. It is not used as an antiseptic. Staff use this very sparingly for cleaning optics. There is no methanol available where general users may access it.

People need to be told repeatedly not to spray directly onto the microscope. Demonstration training is necessary for people to see how to saturate a Kimwipe and how to wipe once and dispose. Perhaps the single most misunderstood aspect of cleaning is that the first wipe removes and the second wipe or circular motion smears it around. I try to explain this with analogies such as proper wiping when changing an infant's diaper, the pink stuff in The Cat In The Hat Comes Back, or fine art painting wet-in-wet, but these references are often not shared by our clients. (This is similar to the training how to clean oil off immersion lenses.)

Use of gloves is another big problem, but here are some highlights. Environmental Health and Safety has clear rules that the microscopes may not be used with gloves on. One argument is that prohibiting gloves makes people more cognizant of safe handling. Regardless, this blanket prohibition removes our having to argue with people over glove use. The main point is that if the samples are too dangerous to use with gloves off, then they can't be used on the microscope. However, cleaning up spills and handling cleaning solvents may require use of gloves. The final cleaning should be such that it is safe for anyone to touch without gloves. People really do need to be reminded that other people will be using the resources after them and there is a responsibility to make it safe for the next users. We supply gloves in the room. We keep the rooms stocked with gloves from small to X-large. People will reuse the same gloves if you don't give them a surplus of gloves. People need to be explicitly taught to peel off gloves inside-out and toss in trash or they will neatly pull off each finger and lay the contaminated glove on a table for reuse.

Any instrument should be clean for immediate subsequent use by someone else.
However, time is also decontamination for many pathogens.

Whereas these points are part of routine BSL-2 protocols, they could be extended to protocol(s) for wiping down exterior of equipment, ancillary equipment including keyboards, mice, mouse pads, furniture such as tables, chairs, doorknobs, the spot on the door everyone pushes on (look at an angle to find the worn or dirty spot), etc.

If you or specific staff are going to do the decontamination, then most of the points above are unnecessary. If unsupervised users will be directed to clean up after each use, I recommend heeding the above. All training would need to be demonstration. Ultimately, if you want decontamination done strictly your way, you need to do it yourself.

(BTW, this email was written in New Rochelle, NY, two miles from where Thomas Paine wrote Common Sense.)

Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016

[hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/

Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of tom.dimatteo <[hidden email]>
Sent: Thursday, March 12, 2020 5:08 PM
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

[EXTERNAL]

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Perhaps temporarily removing the rubber eye cups from the eyepieces would better facilitate cleaning between users for now?Sent from my Verizon, Samsung Galaxy smartphone
-------- Original message --------From: Louis Kerr <[hidden email]> Date: 3/12/20 12:30 PM (GMT-08:00) To: [hidden email] Subject: Re: COVID19: Instrument cleaning protocols *****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.*****I need to add a correction to my earlier statement about the concentration of hydrogen peroxide. The stock is 8% but is diluted to either 0.25 or 0.375%. We also use a health care variety for other uses that is 0.5%.Thanks again,LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email]<mailto:[hidden email]> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= >________________________________From: Louis Kerr <[hidden email]>Sent: Wednesday, March 11, 2020 2:19 PMTo: Glen MacDonald <[hidden email]>; [hidden email] <[hidden email]>Subject: Re: COVID19: Instrument cleaning protocolsI agree that >60% Ethanol degrades the finish and plastics/rubber over time. We have routinely used Ecolabs Peroxide Multi Surface Cleaner and Disinfectant. It contains 8% Hydrogen Peroxide.Very useful discussion as I am currently writing up internal recommendations as well.LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email]<mailto:[hidden email]> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= >________________________________From: Confocal Microscopy List <[hidden email]> on behalf of Glen MacDonald <[hidden email]>Sent: Wednesday, March 11, 2020 1:53 PMTo: [hidden email] <[hidden email]>Subject: Re: COVID19: Instrument cleaning protocols*****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.*****Has anyone tried other disinfectants such as 3% peroxide? 70% EtOH tends to remove some paints, decals and rubber/plastic finishes.Great discussion,Glen> On Mar 11, 2020, at 10:00 AM, Cesare|ALEMBIC <[hidden email]> wrote:>> *****> To join, leave or search the confocal microscopy listserv, go to:> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.> *****>> Yes Martin,>> we use 70% Ethanol for eye-pieces like for each workspace (computer+microscope+devices,etc.)>> They recommended to us considering its volatility and fire point having to use (relatively) "a lot" in a (relatively) small room.>> Best,>> Cesare>>> Il 11/03/2020 17:40, Martin Wessendorf ha scritto:>> *****>> To join, leave or search the confocal microscopy listserv, go to:>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= >> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.>> *****>>>> Dario/Cesare-->>>> THANK YOU BOTH very much for emphasizing the importance of decontaminating>> the eye-pieces. It's obvious in retrospect but I was focussed on the>> computer keyboard, mouse, etc. --Are you using 80% ethanol on the>> eye-pieces?>>>> Best wishes to you and all our other colleagues. Good luck. Please keep>> us posted on developments or recommendations.>>>> And thank you to all those working to control this beast.>>>> Martin Wessendorf>>>> On Wed, Mar 11, 2020 at 8:47 AM Cesare|ALEMBIC <[hidden email]> wrote:>>>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.>>> *****>>>>>> Dear Javier,>>>>>> here in Milano, as already mentioned by Mario and Dario, we are still>>> available (althouhg at lower level as well) but we implemented, according>>> to Institute Direction, different measures to reduce as much as possibile>>> the access to the Facility and we recommended to our Users to perform only>>> urgent and undelayable experiments.>>>>>> Facility Staff:>>> we reduced staff members daily present to the minimum is possible>>> Facility entrance:>>> - Instruments will be available only from 08:00 to 18:00 from Monday to>>> Friday (instead 24/24h, 7/7d) and instrument usage is not allowed on>>> holidays and outside of this time schedule.>>> - A disposable glove dispenser has been set up outside the entrance door>>> of the facility. All Users must wear them before entering the Facility and>>> leave them in a dedicated container as they finish their activities.>>> - the entrance to the Facility will be regulated in order to avoid any>>> form of crowding that sees multiple subjects in the same room at a distance>>> of less than 1.8 meters.>>> - Instruments use, including microscopes mouse and keyboards, must be done>>> by wearing gloves and, as much as possible, with the own protective glasses>>> (please, remember that Covid-19 seems particularly transmitted by aerosol>>> contact with conjunctive).>>> - At the end of each session, each User must notify it to the Facility>>> Staff who will disinfect the exposed parts with Ethanol 70% (microscope,>>> mouse, keyboards, touchscreens etc. all parts that could be usually touched>>> by hands or eyes, using a disposable cloths.>>> - we require only one person per Instrument and, for the same reason,>>> trainings and assisted uses are temporarily suspended.>>>>>>>>> Instruments maintenance :>>> no different procedures respect usual maintenance cleaning>>>>>> I hope this can be useful and a special hello to my "Milanesi" collegues!>>>>>> Cesare>>>>>>>>> Dr. Cesare Covino, M.Sc.>>> Facility Manager>>> ALEMBIC>>> Advanced Light and Electron Microscopy BioImaging Center>>> San Raffaele Scientific Institute>>> DIBIT 1 S2B4a>>> via Olgettina 58, 20132 - Milano - Italy>>> Tel +39-022643-4636(others -4640, -4641)>>> Fax +39-022643-4646>>> e-mail: [hidden email]<mailto:[hidden email]>>>> ALEMBIC webpage: https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e= >>>>>>>>> Il 11/03/2020 10:39, F Javier Diez Guerra ha scritto:>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.>>> *****>>>>>> Dear listers,>>>>>> In Madrid, teaching and many other collective activities have been>>> suspended for 15 days because of COVID19. On the other hand, research>>> institutes continue with their normal activity although at a lower level.>>>>>> In order to reduce as much as possible the spread of the virus, our>>> advanced bioimaging facility is considering to implement additional>>> measures or protocols (beyond common sense).>>>>>> We would be very grateful if you could share your advice, suggestions and>>> protocols especially regarding the cleaning procedures of the instruments>>> (binocular, focus knobs, switches, stage joysticks, etc) and other measures>>> that affect to the users (wear gloves, etc). We are interested to know>>> about easy and effective cleaning procedures that would not be harmful for>>> the optics, electronics, etc.>>>>>> Thanks for your input>>>>>> Best regards>>>>>>>>>>>>>>>>>> Rispetta l'ambiente: non stampare questa mail se non ? necessario.>>> Respect the environment: print this email only if necessary.>>>>> .

Gaurav Joshi

Mar 15, 2020; 8:25pm

Re: COVID19: Instrument cleaning protocols

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Hi everyone,

Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>

It encompasses suggestions put forward by many of you in this discussion thread

Stay safe.

Best,
Gaurav Joshi, PhD <> | Imaging Specialist
Health Sciences Research Building (HSRB)
E-G72, 1760 Haygood Drive, Atlanta, GA 30322

[hidden email] <mailto:[hidden email]>
ici.emory.edu <http://www.cores.emory.edu/ici/>

> On Mar 15, 2020, at 4:14 PM, Jacqueline Ross <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy <http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> Post images on http://www.imgur.com <http://www.imgur.com/> and include the link in your posting.
> *****
>
> Hi everyone,
>
> This has been interesting reading. We currently have 8 confirmed and 3 probable cases in New Zealand. I know this is a very small number compared with many other countries and our sympathies are with you all. However, winter is coming and our government has now stepped up with stronger restrictions. Our university has banned all international travel and recalled all staff who are currently overseas.
>
> In regard to our core facility, I intend to send an email to all microscope users reminding them that they must wash their hands thoroughly with soap (preferred option) or use hand sanitizer before leaving the lab. This is our normal practice.
>
> However I will also be asking all users to also wash their hands before using the microscopes, i.e. after entering the main lab. Based on these discussions, I intend to also ask people to clean the eyepieces before and after using the microscope.
>
> I am also considering removing all plastic/rubber eye cups from the eyepieces as an extra precaution. What do people think about that idea?
>
> Like Michael, we have a "no gloves rule" apart from when people are working with live samples. They can wear gloves when handling their specimen but only when putting it onto and off the microscope
> Gloves must be removed before touching any other components such as focus wheel, joy stick, etc.
>
> For us, it's not practicable for us to clean microscopes between users and I wouldn't feel comfortable leaving anything like bleach around in case some of the more anxious users decided to spray it around.
>
> We use Accel wipes (peroxide) for benches so I will probably wipe those myself each day and also all door handles. However if everyone observes good hand hygiene, the internal room handles should be low risk.
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Lead Technologist
> Optical Microscopy & Image Analysis
> Biomedical Imaging Research Unit (BIRU)
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland 1142, NEW ZEALAND
>
> Telephone: Ext 87438; DDI: +64 9 923 7438
>
> Website: http://www.auckland.ac.nz/biru <http://www.auckland.ac.nz/biru>
>
>
>
>
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email] <mailto:[hidden email]>] on behalf of Cammer, Michael [[hidden email] <mailto:[hidden email]>]
> Sent: 15 March 2020 08:26
> To: [hidden email] <mailto:[hidden email]>
> Subject: Re: COVID19: Instrument cleaning protocols
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I have read many, but not all of, the messages in this thread, so apologies if some redundancy.
>
> The following response is a few tips for microscope decontamination for BSL-2 (and in some cases BSL-3), not specifically for the corona virus, but I believe these points apply and some of them are for equipment protection while also fulfilling the goal of microorganism or virus decontamination. They could be extended to decontamination of scopes and ancillary equipment as per users who may be carrying contagious illnesses. I am writing from the point of view of a core facility with scopes used by many people and in scattered locations with minimal or no supervision. These are more practical tips we have learned (the hard way) for operating as part of more thorough protocols.
>
> Paper goods: We do not allow commercial tissues in the lab. We have had people with colds come to use the microscopes and bring their own box of tissues with them to wipe their noses while using our equipment. (Yes, this is true.) They will use these tissues on the equipment if they are within reach. Tissues are likely to scratch optics and leave dust everywhere. Paper goods need to be limited to lens tissue and wood fiber only Kimwipes.
>
> 70% EtOH and 10% bleach must be kept in bottles that look completely different. People will get them confused otherwise and bleach sprayed on optics is not good. A bottle with a white top and a bottle with a red top with Sharpie marker writing on the side are not different enough. We solve this problem by supplying the 70% EtOH in clear hand labeled bottles and the bleach in prepackaged Clorox bottles. (The clear bottles are marked with Sharpie and then clear packing tape over the writing to prevent the ink from being washed off because, like immersion oil somehow getting applied to every surface, EtOH will run down the side of the bottle. The Clorox bottles are also labeled in BIG letters, Microscopy, to reduce "borrowing" of them; when you need the bleach, you need it next to the scope, not in somebody else's lab.)
>
> Using prepackaged bleach has another benefit. It is buffered so it remains active longer. The downside of this is that it may leave a fine power residue. However, this is preferential to having people mistaking bottles and spraying bleach on optics. The surfaces may be wiped down a second time with EtOH, water, or lens cleaner to remove the powder.
>
> 70% EtOH may damage plastics (other people have mentioned this). Beware overuse on Plexiglas boxes, plastic knobs, and painted surfaces. We have had environmental chambers on scopes develop networks of microcracks due to too much EtOH. If you want to decontaminate these surfaces, we recommend 10% bleach.
>
> Other people have mentioned methanol and its toxicity. It is not used as an antiseptic. Staff use this very sparingly for cleaning optics. There is no methanol available where general users may access it.
>
> People need to be told repeatedly not to spray directly onto the microscope. Demonstration training is necessary for people to see how to saturate a Kimwipe and how to wipe once and dispose. Perhaps the single most misunderstood aspect of cleaning is that the first wipe removes and the second wipe or circular motion smears it around. I try to explain this with analogies such as proper wiping when changing an infant's diaper, the pink stuff in The Cat In The Hat Comes Back, or fine art painting wet-in-wet, but these references are often not shared by our clients. (This is similar to the training how to clean oil off immersion lenses.)
>
> Use of gloves is another big problem, but here are some highlights. Environmental Health and Safety has clear rules that the microscopes may not be used with gloves on. One argument is that prohibiting gloves makes people more cognizant of safe handling. Regardless, this blanket prohibition removes our having to argue with people over glove use. The main point is that if the samples are too dangerous to use with gloves off, then they can't be used on the microscope. However, cleaning up spills and handling cleaning solvents may require use of gloves. The final cleaning should be such that it is safe for anyone to touch without gloves. People really do need to be reminded that other people will be using the resources after them and there is a responsibility to make it safe for the next users. We supply gloves in the room. We keep the rooms stocked with gloves from small to X-large. People will reuse the same gloves if you don't give them a surplus of gloves. People need to be explicitly taught to peel off gloves inside-out and toss in trash or they will neatly pull off each finger and lay the contaminated glove on a table for reuse.
>
> Any instrument should be clean for immediate subsequent use by someone else.
> However, time is also decontamination for many pathogens.
>
> Whereas these points are part of routine BSL-2 protocols, they could be extended to protocol(s) for wiping down exterior of equipment, ancillary equipment including keyboards, mice, mouse pads, furniture such as tables, chairs, doorknobs, the spot on the door everyone pushes on (look at an angle to find the worn or dirty spot), etc.
>
> If you or specific staff are going to do the decontamination, then most of the points above are unnecessary. If unsupervised users will be directed to clean up after each use, I recommend heeding the above. All training would need to be demonstration. Ultimately, if you want decontamination done strictly your way, you need to do it yourself.
>
> (BTW, this email was written in New Rochelle, NY, two miles from where Thomas Paine wrote Common Sense.)
>
> Cheers-
>
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
>
> [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/
>
> Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
>
>
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of tom.dimatteo <[hidden email]>
> Sent: Thursday, March 12, 2020 5:08 PM
> To: [hidden email]
> Subject: Re: COVID19: Instrument cleaning protocols
>
> [EXTERNAL]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.
> *****
>
> Perhaps temporarily removing the rubber eye cups from the eyepieces would better facilitate cleaning between users for now?Sent from my Verizon, Samsung Galaxy smartphone
> -------- Original message --------From: Louis Kerr <[hidden email] <mailto:[hidden email]>> Date: 3/12/20 12:30 PM (GMT-08:00) To: [hidden email] <mailto:[hidden email]> Subject: Re: COVID19: Instrument cleaning protocols *****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= <http://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e=> images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.*****I need to add a correction to my earlier statement about the concentration of hydrogen peroxide. The stock is 8% but is diluted to either 0.25 or 0.375%. We also use a health care variety for other uses that is 0.5%.Thanks again,LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e=><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e=>>________________________________From: Louis Kerr <[hidden email] <mailto:[hidden email]>>Sent: Wednesday, March 11, 2020 2:19 PMTo: Glen MacDonald <[hidden email] <mailto:[hidden email]>>; [hidden email] <mailto:[hidden email]><[hidden email] <mailto:[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocolsI agree that >60% Ethanol degrades the finish and plastics/rubber over time. We have routinely used Ecolabs Peroxide Multi Surface Cleaner and Disinfectant. It contains 8% Hydrogen Peroxide.Very useful discussion as I am currently writing up internal recommendations as well.LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e=><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e=>>________________________________From: Confocal Microscopy List <[hidden email] <mailto:[hidden email]>> on behalf of Glen MacDonald <[hidden email] <mailto:[hidden email]>>Sent: Wednesday, March 11, 2020 1:53 PMTo: [hidden email] <mailto:[hidden email]><[hidden email] <mailto:[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocols*****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= <http://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e=> images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.*****Has anyone tried other disinfectants such as 3% peroxide? 70% EtOH tends to remove some paints, decals and rubber/plastic finishes.Great discussion,Glen> On Mar 11, 2020, at 10:00 AM, Cesare|ALEMBIC <[hidden email] <mailto:[hidden email]>> wrote:>> *****> To join, leave or search the confocal microscopy listserv, go to:> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.> *****>> Yes Martin,>> we use 70% Ethanol for eye-pieces like for each workspace (computer+microscope+devices,etc.)>> They recommended to us considering its volatility and fire point having to use (relatively) "a lot" in a (relatively) small room.>> Best,>> Cesare>>> Il 11/03/2020 17:40, Martin Wessendorf ha scritto:>> *****>> To join, leave or search the confocal microscopy listserv, go to:>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>> *****>>>> Dario/Cesare-->>>> THANK YOU BOTH very much for emphasizing the importance of decontaminating>> the eye-pieces. It's obvious in retrospect but I was focussed on the>> computer keyboard, mouse, etc. --Are you using 80% ethanol on the>> eye-pieces?>>>> Best wishes to you and all our other colleagues. Good luck. Please keep>> us posted on developments or recommendations.>>>> And thank you to all those working to control this beast.>>>> Martin Wessendorf>>>> On Wed, Mar 11, 2020 at 8:47 AM Cesare|ALEMBIC <[hidden email] <mailto:[hidden email]>> wrote:>>>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear Javier,>>>>>> here in Milano, as already mentioned by Mario and Dario, we are still>>> available (althouhg at lower level as well) but we implemented, according>>> to Institute Direction, different measures to reduce as much as possibile>>> the access to the Facility and we recommended to our Users to perform only>>> urgent and undelayable experiments.>>>>>> Facility Staff:>>> we reduced staff members daily present to the minimum is possible>>> Facility entrance:>>> - Instruments will be available only from 08:00 to 18:00 from Monday to>>> Friday (instead 24/24h, 7/7d) and instrument usage is not allowed on>>> holidays and outside of this time schedule.>>> - A disposable glove dispenser has been set up outside the entrance door>>> of the facility. All Users must wear them before entering the Facility and>>> leave them in a dedicated container as they finish their activities.>>> - the entrance to the Facility will be regulated in order to avoid any>>> form of crowding that sees multiple subjects in the same room at a distance>>> of less than 1.8 meters.>>> - Instruments use, including microscopes mouse and keyboards, must be done>>> by wearing gloves and, as much as possible, with the own protective glasses>>> (please, remember that Covid-19 seems particularly transmitted by aerosol>>> contact with conjunctive).>>> - At the end of each session, each User must notify it to the Facility>>> Staff who will disinfect the exposed parts with Ethanol 70% (microscope,>>> mouse, keyboards, touchscreens etc. all parts that could be usually touched>>> by hands or eyes, using a disposable cloths.>>> - we require only one person per Instrument and, for the same reason,>>> trainings and assisted uses are temporarily suspended.>>>>>>>>> Instruments maintenance :>>> no different procedures respect usual maintenance cleaning>>>>>> I hope this can be useful and a special hello to my "Milanesi" collegues!>>>>>> Cesare>>>>>>>>> Dr. Cesare Covino, M.Sc.>>> Facility Manager>>> ALEMBIC>>> Advanced Light and Electron Microscopy BioImaging Center>>> San Raffaele Scientific Institute>>> DIBIT 1 S2B4a>>> via Olgettina 58, 20132 - Milano - Italy>>> Tel +39-022643-4636(others -4640, -4641)>>> Fax +39-022643-4646>>> e-mail: [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>>>>> ALEMBIC webpage: https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e=> >>>>>>>>> Il 11/03/2020 10:39, F Javier Diez Guerra ha scritto:>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear listers,>>>>>> In Madrid, teaching and many other collective activities have been>>> suspended for 15 days because of COVID19. On the other hand, research>>> institutes continue with their normal activity although at a lower level.>>>>>> In order to reduce as much as possible the spread of the virus, our>>> advanced bioimaging facility is considering to implement additional>>> measures or protocols (beyond common sense).>>>>>> We would be very grateful if you could share your advice, suggestions and>>> protocols especially regarding the cleaning procedures of the instruments>>> (binocular, focus knobs, switches, stage joysticks, etc) and other measures>>> that affect to the users (wear gloves, etc). We are interested to know>>> about easy and effective cleaning procedures that would not be harmful for>>> the optics, electronics, etc.>>>>>> Thanks for your input>>>>>> Best regards>>>>>>>>>>>>>>>>>> Rispetta l'ambiente: non stampare questa mail se non ? necessario.>>> Respect the environment: print this email only if necessary.>>>>> .

... [show rest of quote]

Jacqueline Ross

Mar 15, 2020; 8:46pm

Re: COVID19: Instrument cleaning protocols

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*****

Dear Gaurav,

Thank you very much for sharing this with us. It's very nice and graphical😊.

I just have a couple of quick questions for you/your colleague.

What "disinfecting" wipes are you using for the joysticks/focus wheel/computer peripherals? Are they peroxide-based or ethanol/isopropyl alcohol-based? I don't think we would be able to use wipes at this level due to lack of supplies. People would need to spray onto paper towel.

Also, any thoughts on removing eye cups?

Kind regards,

Jacqui

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Gaurav Joshi
Sent: Monday, March 16, 2020 9:26 AM
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

*****
To join, leave or search the confocal microscopy listserv, go to:
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Hi everyone,

Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>

It encompasses suggestions put forward by many of you in this discussion thread

Stay safe.

Best,
Gaurav Joshi, PhD <> | Imaging Specialist
Health Sciences Research Building (HSRB)
E-G72, 1760 Haygood Drive, Atlanta, GA 30322

[hidden email] <mailto:[hidden email]>
ici.emory.edu <http://www.cores.emory.edu/ici/>

... [show rest of quote]

0000001ed7f52e4a-dmarc-request

Mar 15, 2020; 10:54pm

Re: COVID19: Instrument cleaning protocols

In reply to this post by Gaurav Joshi

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Thanks Gustav for sharing and everyone for the good contributions, great to have this important discussion.
I don't understand the no-gloves rule in the times of Covid-19, it assumes that the gloves are contaminated and the hands are clean. But now we are facing the situation that the microscope, keyboard, mouse can be dirty and people want to keep their hands clean. I think it should be gloves only policy. When using no-gloves would rely on everyone following the rules perfectly, one infected person coughing into their hand and touching the mouse or focus knob could spread the disease. Just my thoughts.
stay safe ad take care
Andreas

-----Original Message-----
From: Gaurav Joshi <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Sun, 15 Mar 2020 20:26
Subject: Re: COVID19: Instrument cleaning protocols

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi everyone,

Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>

It encompasses suggestions put forward by many of you in this discussion thread

Stay safe.

Best,
Gaurav Joshi, PhD <> | Imaging Specialist
Health Sciences Research Building (HSRB)
E-G72, 1760 Haygood Drive, Atlanta, GA 30322

[hidden email] <mailto:[hidden email]>
ici.emory.edu <http://www.cores.emory.edu/ici/>

... [show rest of quote]

Gaurav Joshi

Mar 16, 2020; 12:00am

Re: COVID19: Instrument cleaning protocols

In reply to this post by Jacqueline Ross

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Post images on http://www.imgur.com and include the link in your posting.
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Hi Jacqui and Andreas,

Jacqui, we are using Clorox disinfecting wipes. These are isopropyl alcohol based wipes. Since we have them we are recommending the use of these wipes. Alternately, 70% ethanol on a paper towel should be fine as suggested by others on this thread. I do not expect users to remove eye cups. The hope is that rubber will not crack while the new protocol is implemented for few months. Worst case scenario, if they crack we will have to buy new ones.

Andreas, your suggestion is definitely an alternative. Since we have sinks, we are asking users to wash their hands with soap and then work on instruments. Emory just like other institutions has emphasized on frequent hand washing.

While we expect users to maintain a proper etiquette in such times, as facility scientists we make sure to oversee their habits and intervene appropriately.

Always happy to hear thoughts from this group.

Stay safe.

Best,
Gaurav.

> On Mar 15, 2020, at 4:46 PM, Jacqueline Ross <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Gaurav,
>
> Thank you very much for sharing this with us. It's very nice and graphical😊.
>
> I just have a couple of quick questions for you/your colleague.
>
> What "disinfecting" wipes are you using for the joysticks/focus wheel/computer peripherals? Are they peroxide-based or ethanol/isopropyl alcohol-based? I don't think we would be able to use wipes at this level due to lack of supplies. People would need to spray onto paper towel.
>
> Also, any thoughts on removing eye cups?
>
> Kind regards,
>
> Jacqui
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Gaurav Joshi
> Sent: Monday, March 16, 2020 9:26 AM
> To: [hidden email]
> Subject: Re: COVID19: Instrument cleaning protocols
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everyone,
>
> Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
> https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>
>
> It encompasses suggestions put forward by many of you in this discussion thread
>
>
> Stay safe.
>
> Best,
> Gaurav Joshi, PhD <> | Imaging Specialist
> Health Sciences Research Building (HSRB)
> E-G72, 1760 Haygood Drive, Atlanta, GA 30322
>
> [hidden email] <mailto:[hidden email]>
> ici.emory.edu <http://www.cores.emory.edu/ici/>
>
>
>
>> On Mar 15, 2020, at 4:14 PM, Jacqueline Ross <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy <http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> Post images on http://www.imgur.com <http://www.imgur.com/> and include the link in your posting.
>> *****
>>
>> Hi everyone,
>>
>> This has been interesting reading. We currently have 8 confirmed and 3 probable cases in New Zealand. I know this is a very small number compared with many other countries and our sympathies are with you all. However, winter is coming and our government has now stepped up with stronger restrictions. Our university has banned all international travel and recalled all staff who are currently overseas.
>>
>> In regard to our core facility, I intend to send an email to all microscope users reminding them that they must wash their hands thoroughly with soap (preferred option) or use hand sanitizer before leaving the lab. This is our normal practice.
>>
>> However I will also be asking all users to also wash their hands before using the microscopes, i.e. after entering the main lab. Based on these discussions, I intend to also ask people to clean the eyepieces before and after using the microscope.
>>
>> I am also considering removing all plastic/rubber eye cups from the eyepieces as an extra precaution. What do people think about that idea?
>>
>> Like Michael, we have a "no gloves rule" apart from when people are working with live samples. They can wear gloves when handling their specimen but only when putting it onto and off the microscope
>> Gloves must be removed before touching any other components such as focus wheel, joy stick, etc.
>>
>> For us, it's not practicable for us to clean microscopes between users and I wouldn't feel comfortable leaving anything like bleach around in case some of the more anxious users decided to spray it around.
>>
>> We use Accel wipes (peroxide) for benches so I will probably wipe those myself each day and also all door handles. However if everyone observes good hand hygiene, the internal room handles should be low risk.
>>
>> Kind regards,
>>
>> Jacqui
>>
>> Jacqueline Ross
>> Lead Technologist
>> Optical Microscopy & Image Analysis
>> Biomedical Imaging Research Unit (BIRU)
>> School of Medical Sciences
>> Faculty of Medical & Health Sciences
>> The University of Auckland
>> Private Bag 92019
>> Auckland 1142, NEW ZEALAND
>>
>> Telephone: Ext 87438; DDI: +64 9 923 7438
>>
>> Website: http://www.auckland.ac.nz/biru <http://www.auckland.ac.nz/biru>
>>
>>
>>
>>
>>
>> ________________________________________
>> From: Confocal Microscopy List [[hidden email] <mailto:[hidden email]>] on behalf of Cammer, Michael [[hidden email] <mailto:[hidden email]>]
>> Sent: 15 March 2020 08:26
>> To: [hidden email] <mailto:[hidden email]>
>> Subject: Re: COVID19: Instrument cleaning protocols
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I have read many, but not all of, the messages in this thread, so apologies if some redundancy.
>>
>> The following response is a few tips for microscope decontamination for BSL-2 (and in some cases BSL-3), not specifically for the corona virus, but I believe these points apply and some of them are for equipment protection while also fulfilling the goal of microorganism or virus decontamination. They could be extended to decontamination of scopes and ancillary equipment as per users who may be carrying contagious illnesses. I am writing from the point of view of a core facility with scopes used by many people and in scattered locations with minimal or no supervision. These are more practical tips we have learned (the hard way) for operating as part of more thorough protocols.
>>
>> Paper goods: We do not allow commercial tissues in the lab. We have had people with colds come to use the microscopes and bring their own box of tissues with them to wipe their noses while using our equipment. (Yes, this is true.) They will use these tissues on the equipment if they are within reach. Tissues are likely to scratch optics and leave dust everywhere. Paper goods need to be limited to lens tissue and wood fiber only Kimwipes.
>>
>> 70% EtOH and 10% bleach must be kept in bottles that look completely different. People will get them confused otherwise and bleach sprayed on optics is not good. A bottle with a white top and a bottle with a red top with Sharpie marker writing on the side are not different enough. We solve this problem by supplying the 70% EtOH in clear hand labeled bottles and the bleach in prepackaged Clorox bottles. (The clear bottles are marked with Sharpie and then clear packing tape over the writing to prevent the ink from being washed off because, like immersion oil somehow getting applied to every surface, EtOH will run down the side of the bottle. The Clorox bottles are also labeled in BIG letters, Microscopy, to reduce "borrowing" of them; when you need the bleach, you need it next to the scope, not in somebody else's lab.)
>>
>> Using prepackaged bleach has another benefit. It is buffered so it remains active longer. The downside of this is that it may leave a fine power residue. However, this is preferential to having people mistaking bottles and spraying bleach on optics. The surfaces may be wiped down a second time with EtOH, water, or lens cleaner to remove the powder.
>>
>> 70% EtOH may damage plastics (other people have mentioned this). Beware overuse on Plexiglas boxes, plastic knobs, and painted surfaces. We have had environmental chambers on scopes develop networks of microcracks due to too much EtOH. If you want to decontaminate these surfaces, we recommend 10% bleach.
>>
>> Other people have mentioned methanol and its toxicity. It is not used as an antiseptic. Staff use this very sparingly for cleaning optics. There is no methanol available where general users may access it.
>>
>> People need to be told repeatedly not to spray directly onto the microscope. Demonstration training is necessary for people to see how to saturate a Kimwipe and how to wipe once and dispose. Perhaps the single most misunderstood aspect of cleaning is that the first wipe removes and the second wipe or circular motion smears it around. I try to explain this with analogies such as proper wiping when changing an infant's diaper, the pink stuff in The Cat In The Hat Comes Back, or fine art painting wet-in-wet, but these references are often not shared by our clients. (This is similar to the training how to clean oil off immersion lenses.)
>>
>> Use of gloves is another big problem, but here are some highlights. Environmental Health and Safety has clear rules that the microscopes may not be used with gloves on. One argument is that prohibiting gloves makes people more cognizant of safe handling. Regardless, this blanket prohibition removes our having to argue with people over glove use. The main point is that if the samples are too dangerous to use with gloves off, then they can't be used on the microscope. However, cleaning up spills and handling cleaning solvents may require use of gloves. The final cleaning should be such that it is safe for anyone to touch without gloves. People really do need to be reminded that other people will be using the resources after them and there is a responsibility to make it safe for the next users. We supply gloves in the room. We keep the rooms stocked with gloves from small to X-large. People will reuse the same gloves if you don't give them a surplus of gloves. People need to be explicitly taught to peel off gloves inside-out and toss in trash or they will neatly pull off each finger and lay the contaminated glove on a table for reuse.
>>
>> Any instrument should be clean for immediate subsequent use by someone else.
>> However, time is also decontamination for many pathogens.
>>
>> Whereas these points are part of routine BSL-2 protocols, they could be extended to protocol(s) for wiping down exterior of equipment, ancillary equipment including keyboards, mice, mouse pads, furniture such as tables, chairs, doorknobs, the spot on the door everyone pushes on (look at an angle to find the worn or dirty spot), etc.
>>
>> If you or specific staff are going to do the decontamination, then most of the points above are unnecessary. If unsupervised users will be directed to clean up after each use, I recommend heeding the above. All training would need to be demonstration. Ultimately, if you want decontamination done strictly your way, you need to do it yourself.
>>
>> (BTW, this email was written in New Rochelle, NY, two miles from where Thomas Paine wrote Common Sense.)
>>
>> Cheers-
>>
>>
>>
>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>>
>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
>>
>> [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/
>>
>> Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
>>
>>
>>
>> ________________________________
>> From: Confocal Microscopy List <[hidden email]> on behalf of tom.dimatteo <[hidden email]>
>> Sent: Thursday, March 12, 2020 5:08 PM
>> To: [hidden email]
>> Subject: Re: COVID19: Instrument cleaning protocols
>>
>> [EXTERNAL]
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=
>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= and include the link in your posting.
>> *****
>>
>> Perhaps temporarily removing the rubber eye cups from the eyepieces would better facilitate cleaning between users for now?Sent from my Verizon, Samsung Galaxy smartphone
>> -------- Original message --------From: Louis Kerr <[hidden email] <mailto:[hidden email]>> Date: 3/12/20 12:30 PM (GMT-08:00) To: [hidden email] <mailto:[hidden email]> Subject: Re: COVID19: Instrument cleaning protocols *****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= <http://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e=> images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.*****I need to add a correction to my earlier statement about the concentration of hydrogen peroxide. The stock is 8% but is diluted to either 0.25 or 0.375%. We also use a health care variety for other uses that is 0.5%.Thanks again,LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e=><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e=>>________________________________From: Louis Kerr <[hidden email] <mailto:[hidden email]>>Sent: Wednesday, March 11, 2020 2:19 PMTo: Glen MacDonald <[hidden email] <mailto:[hidden email]>>; [hidden email] <mailto:[hidden email]><[hidden email] <mailto:[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocolsI agree that >60% Ethanol degrades the finish and plastics/rubber over time. We have routinely used Ecolabs Peroxide Multi Surface Cleaner and Disinfectant. It contains 8% Hydrogen Peroxide.Very useful discussion as I am currently writing up internal recommendations as well.LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 | [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=FPJ3-smFjgsdZorvJ7H4ndYdr1s4SdkmLGxupEIGNyI&e=><https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mbl.edu_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=ojtYdwBF_VuA8dI044tvhC0egxY4Ur3CUAxzhf9X98s&e=>>________________________________From: Confocal Microscopy List <[hidden email] <mailto:[hidden email]>> on behalf of Glen MacDonald <[hidden email] <mailto:[hidden email]>>Sent: Wednesday, March 11, 2020 1:53 PMTo: [hidden email] <mailto:[hidden email]><[hidden email] <mailto:[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocols*****To join, leave or search the confocal microscopy listserv, go to:https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e= <http://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3DconfocalmicroscopyPost&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=lLqtCdVm9sCINhkySsvM4LW8fsOl82Fa0j8RDu8tfTU&e=> images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.*****Has anyone tried other disinfectants such as 3% peroxide? 70% EtOH tends to remove some paints, decals and rubber/plastic finishes.Great discussion,Glen> On Mar 11, 2020, at 10:00 AM, Cesare|ALEMBIC <[hidden email] <mailto:[hidden email]>> wrote:>> *****> To join, leave or search the confocal microscopy listserv, go to:> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.> *****>> Yes Martin,>> we use 70% Ethanol for eye-pieces like for each workspace (computer+microscope+devices,etc.)>> They recommended to us considering its volatility and fire point having to use (relatively) "a lot" in a (relatively) small room.>> Best,>> Cesare>>> Il 11/03/2020 17:40, Martin Wessendorf ha scritto:>> *****>> To join, leave or search the confocal microscopy listserv, go to:>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>> *****>>>> Dario/Cesare-->>>> THANK YOU BOTH very much for emphasizing the importance of decontaminating>> the eye-pieces. It's obvious in retrospect but I was focussed on the>> computer keyboard, mouse, etc. --Are you using 80% ethanol on the>> eye-pieces?>>>> Best wishes to you and all our other colleagues. Good luck. Please keep>> us posted on developments or recommendations.>>>> And thank you to all those working to control this beast.>>>> Martin Wessendorf>>>> On Wed, Mar 11, 2020 at 8:47 AM Cesare|ALEMBIC <[hidden email] <mailto:[hidden email]>> wrote:>>>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear Javier,>>>>>> here in Milano, as already mentioned by Mario and Dario, we are still>>> available (althouhg at lower level as well) but we implemented, according>>> to Institute Direction, different measures to reduce as much as possibile>>> the access to the Facility and we recommended to our Users to perform only>>> urgent and undelayable experiments.>>>>>> Facility Staff:>>> we reduced staff members daily present to the minimum is possible>>> Facility entrance:>>> - Instruments will be available only from 08:00 to 18:00 from Monday to>>> Friday (instead 24/24h, 7/7d) and instrument usage is not allowed on>>> holidays and outside of this time schedule.>>> - A disposable glove dispenser has been set up outside the entrance door>>> of the facility. All Users must wear them before entering the Facility and>>> leave them in a dedicated container as they finish their activities.>>> - the entrance to the Facility will be regulated in order to avoid any>>> form of crowding that sees multiple subjects in the same room at a distance>>> of less than 1.8 meters.>>> - Instruments use, including microscopes mouse and keyboards, must be done>>> by wearing gloves and, as much as possible, with the own protective glasses>>> (please, remember that Covid-19 seems particularly transmitted by aerosol>>> contact with conjunctive).>>> - At the end of each session, each User must notify it to the Facility>>> Staff who will disinfect the exposed parts with Ethanol 70% (microscope,>>> mouse, keyboards, touchscreens etc. all parts that could be usually touched>>> by hands or eyes, using a disposable cloths.>>> - we require only one person per Instrument and, for the same reason,>>> trainings and assisted uses are temporarily suspended.>>>>>>>>> Instruments maintenance :>>> no different procedures respect usual maintenance cleaning>>>>>> I hope this can be useful and a special hello to my "Milanesi" collegues!>>>>>> Cesare>>>>>>>>> Dr. Cesare Covino, M.Sc.>>> Facility Manager>>> ALEMBIC>>> Advanced Light and Electron Microscopy BioImaging Center>>> San Raffaele Scientific Institute>>> DIBIT 1 S2B4a>>> via Olgettina 58, 20132 - Milano - Italy>>> Tel +39-022643-4636(others -4640, -4641)>>> Fax +39-022643-4646>>> e-mail: [hidden email] <mailto:[hidden email]><mailto:[hidden email] <mailto:[hidden email]>>>>> ALEMBIC webpage: https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e=> >>>>>>>>> Il 11/03/2020 10:39, F Javier Diez Guerra ha scritto:>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear listers,>>>>>> In Madrid, teaching and many other collective activities have been>>> suspended for 15 days because of COVID19. On the other hand, research>>> institutes continue with their normal activity although at a lower level.>>>>>> In order to reduce as much as possible the spread of the virus, our>>> advanced bioimaging facility is considering to implement additional>>> measures or protocols (beyond common sense).>>>>>> We would be very grateful if you could share your advice, suggestions and>>> protocols especially regarding the cleaning procedures of the instruments>>> (binocular, focus knobs, switches, stage joysticks, etc) and other measures>>> that affect to the users (wear gloves, etc). We are interested to know>>> about easy and effective cleaning procedures that would not be harmful for>>> the optics, electronics, etc.>>>>>> Thanks for your input>>>>>> Best regards>>>>>>>>>>>>>>>>>> Rispetta l'ambiente: non stampare questa mail se non ? necessario.>>> Respect the environment: print this email only if necessary.>>>>> .

... [show rest of quote]

Jacqueline Ross

Mar 16, 2020; 12:06am

Re: COVID19: Instrument cleaning protocols

In reply to this post by 0000001ed7f52e4a-dmarc-request

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Dear Andreas,

Thanks for this comment in regard to the use of gloves/no gloves. This is something that my colleagues and I will be discussing further. I'm also currently waiting on our local expert for some advice.

My feeling is that if people observe good hand hygiene before and especially after using the microscopes, this reduces the risk to that of a similar level to using a lift button, opening doors elsewhere in the faculty, etc. The risk is not only in our lab but outside it and we're trying to provide a consistent message.

Kind regards,

Jacqui

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Andreas Bruckbauer
Sent: Monday, March 16, 2020 11:55 AM
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

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Thanks Gustav for sharing and everyone for the good contributions, great to have this important discussion.
I don't understand the no-gloves rule in the times of Covid-19, it assumes that the gloves are contaminated and the hands are clean. But now we are facing the situation that the microscope, keyboard, mouse can be dirty and people want to keep their hands clean. I think it should be gloves only policy. When using no-gloves would rely on everyone following the rules perfectly, one infected person coughing into their hand and touching the mouse or focus knob could spread the disease. Just my thoughts.
stay safe ad take care
Andreas

-----Original Message-----
From: Gaurav Joshi <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Sun, 15 Mar 2020 20:26
Subject: Re: COVID19: Instrument cleaning protocols

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Hi everyone,

Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>

It encompasses suggestions put forward by many of you in this discussion thread

Stay safe.

Best,
Gaurav Joshi, PhD <> | Imaging Specialist
Health Sciences Research Building (HSRB)
E-G72, 1760 Haygood Drive, Atlanta, GA 30322

[hidden email] <mailto:[hidden email]>
ici.emory.edu <http://www.cores.emory.edu/ici/>

... [show rest of quote]

Jacqueline Ross

Mar 16, 2020; 12:07am

Re: COVID19: Instrument cleaning protocols

In reply to this post by Gaurav Joshi

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Thanks Gaurav for the information on the wipes. We are having difficulty in getting supplies here so wipes are probably not going to be possible.

Kind regards,

Jacqui

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Gaurav Joshi
Sent: Monday, March 16, 2020 1:00 PM
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

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Hi Jacqui and Andreas,

Jacqui, we are using Clorox disinfecting wipes. These are isopropyl alcohol based wipes. Since we have them we are recommending the use of these wipes. Alternately, 70% ethanol on a paper towel should be fine as suggested by others on this thread. I do not expect users to remove eye cups. The hope is that rubber will not crack while the new protocol is implemented for few months. Worst case scenario, if they crack we will have to buy new ones.

Andreas, your suggestion is definitely an alternative. Since we have sinks, we are asking users to wash their hands with soap and then work on instruments. Emory just like other institutions has emphasized on frequent hand washing.

While we expect users to maintain a proper etiquette in such times, as facility scientists we make sure to oversee their habits and intervene appropriately.

Always happy to hear thoughts from this group.

Stay safe.

Best,
Gaurav.

... [show rest of quote]

Jacqueline Ross

Mar 16, 2020; 6:15am

Re: COVID19: Instrument cleaning protocols

In reply to this post by Jacqueline Ross

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Dear Andreas and others,

I forgot to say that I have also instructed our users to wash their hands again if they cough or sneeze into their hands or a tissue (they must also discard the tissue) and that they should use a paper towel to open the door to the main lab where the hand basin is so they don't contaminate the door handle on exit....

It's by no means perfect but it's a start.

Kind regards,

Jacqui
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Jacqueline Ross [[hidden email]]
Sent: 16 March 2020 13:06
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

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*****

Dear Andreas,

Thanks for this comment in regard to the use of gloves/no gloves. This is something that my colleagues and I will be discussing further. I'm also currently waiting on our local expert for some advice.

My feeling is that if people observe good hand hygiene before and especially after using the microscopes, this reduces the risk to that of a similar level to using a lift button, opening doors elsewhere in the faculty, etc. The risk is not only in our lab but outside it and we're trying to provide a consistent message.

Kind regards,

Jacqui

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Andreas Bruckbauer
Sent: Monday, March 16, 2020 11:55 AM
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Gustav for sharing and everyone for the good contributions, great to have this important discussion.
I don't understand the no-gloves rule in the times of Covid-19, it assumes that the gloves are contaminated and the hands are clean. But now we are facing the situation that the microscope, keyboard, mouse can be dirty and people want to keep their hands clean. I think it should be gloves only policy. When using no-gloves would rely on everyone following the rules perfectly, one infected person coughing into their hand and touching the mouse or focus knob could spread the disease. Just my thoughts.
stay safe ad take care
Andreas

-----Original Message-----
From: Gaurav Joshi <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Sun, 15 Mar 2020 20:26
Subject: Re: COVID19: Instrument cleaning protocols

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi everyone,

Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view <https://drive.google.com/file/d/127iSriuhpFfCAFI4JSzPI_R2JQjXw0_F/view>

It encompasses suggestions put forward by many of you in this discussion thread

Stay safe.

Best,
Gaurav Joshi, PhD <> | Imaging Specialist
Health Sciences Research Building (HSRB)
E-G72, 1760 Haygood Drive, Atlanta, GA 30322

[hidden email] <mailto:[hidden email]>
ici.emory.edu <http://www.cores.emory.edu/ici/>

... [show rest of quote]

daj1u06

Mar 16, 2020; 10:44am

Re: COVID19: Instrument cleaning protocols

In reply to this post by F Javier Diez Guerra

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I'm with Andreas on this, there may be asymptomatic carriers out there (including unit staff), we just don't know, so have to assume the potential that any part of the scopes that get any human contact could be contaminated, and we consider the scopes and most other ancillary instruments to effectively be impossible to decontaminate without risk of damage, hence fresh gloves on entering the unit, that can be sprayed with 70% ethanol on regular basis, prevents you contaminating or being contaminated. We have confocals, TEMs, SEMs, slide scanners and analysis PCs to name a few and are based in a large teaching hospital site, so are also thinking about trying to encourage remote access to analysis PCs to minimise footfall and movement around the unit and wider site. That being said, have yet to convince the boss to adopt those policies.

On the plus side, given reported viability on surfaces, a first thing in the morning clean and last thing at night clean may be unnecessary as if any virus at end of day lock up, will be inactive by morning? Between user cleaning may be best but we can have several dozen users in at any one time moving between instruments and don't want to give them access to spray bottles!!!!!

Dave Johnston, Biomedical Imaging, Southampton.

Anne Vaahtokari

Mar 16, 2020; 4:36pm

Re: COVID19: Instrument cleaning protocols

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Regarding the eyepieces, I am instructing our users to cover them with Saran wrap which is transparent and does not affect viewing. One user complained that the wrap tickles her eye lashes and makes her want to rub her eyes. If that is an issue, then it is better to wrap each eyepiece individually and use your (clean!) thumb to press the wrap deeper inside the eye caps. After use, the wrap should be immediately discarded, without touching the side that was against the eyepieces.

Anne

From: Dave Johnston<mailto:[hidden email]>
Sent: 16 March 2020 04:44
To: [hidden email]<mailto:[hidden email]>
Subject: Re: COVID19: Instrument cleaning protocols

*****
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I'm with Andreas on this, there may be asymptomatic carriers out there (including unit staff), we just don't know, so have to assume the potential that any part of the scopes that get any human contact could be contaminated, and we consider the scopes and most other ancillary instruments to effectively be impossible to decontaminate without risk of damage, hence fresh gloves on entering the unit, that can be sprayed with 70% ethanol on regular basis, prevents you contaminating or being contaminated. We have confocals, TEMs, SEMs, slide scanners and analysis PCs to name a few and are based in a large teaching hospital site, so are also thinking about trying to encourage remote access to analysis PCs to minimise footfall and movement around the unit and wider site. That being said, have yet to convince the boss to adopt those policies.

On the plus side, given reported viability on surfaces, a first thing in the morning clean and last thing at night clean may be unnecessary as if any virus at end of day lock up, will be inactive by morning? Between user cleaning may be best but we can have several dozen users in at any one time moving between instruments and don't want to give them access to spray bottles!!!!!

Dave Johnston, Biomedical Imaging, Southampton.

Martin Wessendorf-2

Mar 16, 2020; 7:01pm

Re: COVID19: Instrument cleaning protocols

In reply to this post by daj1u06

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Dear Dave--

With regard to viability, the half-life of the virus on stainless steel was
13 hours and on polypropylene was 16 hours. So yes, decontamination at the
end of the day appears to be a good idea.

https://www.medrxiv.org/content/10.1101/2020.03.09.20033217v1.full.pdf

Martin

On Mon, Mar 16, 2020 at 5:44 AM Dave Johnston <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I'm with Andreas on this, there may be asymptomatic carriers out there
> (including unit staff), we just don't know, so have to assume the potential
> that any part of the scopes that get any human contact could be
> contaminated, and we consider the scopes and most other ancillary
> instruments to effectively be impossible to decontaminate without risk of
> damage, hence fresh gloves on entering the unit, that can be sprayed with
> 70% ethanol on regular basis, prevents you contaminating or being
> contaminated. We have confocals, TEMs, SEMs, slide scanners and analysis
> PCs to name a few and are based in a large teaching hospital site, so are
> also thinking about trying to encourage remote access to analysis PCs to
> minimise footfall and movement around the unit and wider site. That being
> said, have yet to convince the boss to adopt those policies.
>
> On the plus side, given reported viability on surfaces, a first thing in
> the morning clean and last thing at night clean may be unnecessary as if
> any virus at end of day lock up, will be inactive by morning? Between user
> cleaning may be best but we can have several dozen users in at any one time
> moving between instruments and don't want to give them access to spray
> bottles!!!!!
>
> Dave Johnston, Biomedical Imaging, Southampton.
>

... [show rest of quote]

Nadine Utz

Mar 17, 2020; 4:08pm

Re: COVID19: Instrument cleaning protocols: Recommendations by German BioImaging

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Dear all,

Core Facility Managers, who attended the "Trends in Microscopy - German
BioImaging Spring School" last week, held last Friday a spontaneous
workshop to discuss precautions of imaging core facilities to prevent
COVID19 infection of staff and users. The outcome of this workshop has
been summarized in recommendations by German BioImaging. I could not
include an attachment to this email and therefor uploaded the document
to this web site:

https://www.gerbi-gmb.de/Corona

I would like to express my sincere thanks to the participants of the
workshop:

Steffen Dietzel, Ludwig Maximilian University of Munich
Hans Fried, German Center for Neurodegenerative Diseases (DZNE) in Bonn
Angela Naumann, University of Freiburg
Roland Nitschke, University of Freiburg
Pawel Pasierbeck, IMBA Institute of Molecular Biotechnology in Vienna
Britta Schroth-Diez, MPI of Molecular Cell Biology and Genetics
Martin Stoeckl, University of Konstanz
Stefan Terjung, European Molecular Biology Laboratory in Heidelberg
Roland Thuenauer, Centre for Structural Systems Biology in Hamburg
Silke Tulok, Technical University of Dresden

Workshop discussions were mainly based on:
- this confocal mailing list discussion
- recommendations by the World Health Organization
- recommendations by the Robert Koch Institute (German federal
government agency and research institute responsible for disease control
and prevention)

Best wishes
Nadine

--
Dr. Nadine Utz
Managing Director

German BioImaging - Gesellschaft für Mikroskopie und Bildanalyse e.V.
German BioImaging - Society for Microscopy and Image Analysis c/o University of Konstanz
Box 604
78457 Konstanz, Germany
Phone +49 7531 885337
[hidden email]
http://www.gerbi-gmb.de

Am 16.03.20 um 20:01 schrieb Martin Wessendorf:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Dave--
>
> With regard to viability, the half-life of the virus on stainless steel was
> 13 hours and on polypropylene was 16 hours. So yes, decontamination at the
> end of the day appears to be a good idea.
>
> https://www.medrxiv.org/content/10.1101/2020.03.09.20033217v1.full.pdf
>
> Martin
>
>
> On Mon, Mar 16, 2020 at 5:44 AM Dave Johnston <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I'm with Andreas on this, there may be asymptomatic carriers out there
>> (including unit staff), we just don't know, so have to assume the potential
>> that any part of the scopes that get any human contact could be
>> contaminated, and we consider the scopes and most other ancillary
>> instruments to effectively be impossible to decontaminate without risk of
>> damage, hence fresh gloves on entering the unit, that can be sprayed with
>> 70% ethanol on regular basis, prevents you contaminating or being
>> contaminated. We have confocals, TEMs, SEMs, slide scanners and analysis
>> PCs to name a few and are based in a large teaching hospital site, so are
>> also thinking about trying to encourage remote access to analysis PCs to
>> minimise footfall and movement around the unit and wider site. That being
>> said, have yet to convince the boss to adopt those policies.
>>
>> On the plus side, given reported viability on surfaces, a first thing in
>> the morning clean and last thing at night clean may be unnecessary as if
>> any virus at end of day lock up, will be inactive by morning? Between user
>> cleaning may be best but we can have several dozen users in at any one time
>> moving between instruments and don't want to give them access to spray
>> bottles!!!!!
>>
>> Dave Johnston, Biomedical Imaging, Southampton.
>>

... [show rest of quote]

Jacqueline Ross

Mar 17, 2020; 7:18pm

Re: COVID19: Instrument cleaning protocols: Recommendations by German BioImaging

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Dear Nadine,

Thanks very much to you and the Core Facilities Managers group for coming up with these valuable recommendations. I still haven't received the advice I was waiting on from our local expert, probably because they are heavily in demand at this time so I very much appreciate this guidance.

In the light of these recommendations, I am rethinking our no gloves rule. I have already implemented the other recommendations except for wrapping the eyepieces in saran wrap. Minimizing training will be difficult for us at this time of year also given the low number of cases so far. However I expect that usage will drop anyway due to anxiety among our researchers and graduate students.

This document will also provide very useful support for our precautionary actions.

Kind regards,

Jacqui
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Nadine Utz [[hidden email]]
Sent: 18 March 2020 05:08
To: [hidden email]
Subject: Re: COVID19: Instrument cleaning protocols: Recommendations by German BioImaging

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Dear all,

Core Facility Managers, who attended the "Trends in Microscopy - German
BioImaging Spring School" last week, held last Friday a spontaneous
workshop to discuss precautions of imaging core facilities to prevent
COVID19 infection of staff and users. The outcome of this workshop has
been summarized in recommendations by German BioImaging. I could not
include an attachment to this email and therefor uploaded the document
to this web site:

https://www.gerbi-gmb.de/Corona

I would like to express my sincere thanks to the participants of the
workshop:

Steffen Dietzel, Ludwig Maximilian University of Munich
Hans Fried, German Center for Neurodegenerative Diseases (DZNE) in Bonn
Angela Naumann, University of Freiburg
Roland Nitschke, University of Freiburg
Pawel Pasierbeck, IMBA Institute of Molecular Biotechnology in Vienna
Britta Schroth-Diez, MPI of Molecular Cell Biology and Genetics
Martin Stoeckl, University of Konstanz
Stefan Terjung, European Molecular Biology Laboratory in Heidelberg
Roland Thuenauer, Centre for Structural Systems Biology in Hamburg
Silke Tulok, Technical University of Dresden

Workshop discussions were mainly based on:
- this confocal mailing list discussion
- recommendations by the World Health Organization
- recommendations by the Robert Koch Institute (German federal
government agency and research institute responsible for disease control
and prevention)

Best wishes
Nadine

--
Dr. Nadine Utz
Managing Director

German BioImaging - Gesellschaft für Mikroskopie und Bildanalyse e.V.
German BioImaging - Society for Microscopy and Image Analysis c/o University of Konstanz
Box 604
78457 Konstanz, Germany
Phone +49 7531 885337
[hidden email]
http://www.gerbi-gmb.de

Am 16.03.20 um 20:01 schrieb Martin Wessendorf:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Dave--
>
> With regard to viability, the half-life of the virus on stainless steel was
> 13 hours and on polypropylene was 16 hours. So yes, decontamination at the
> end of the day appears to be a good idea.
>
> https://www.medrxiv.org/content/10.1101/2020.03.09.20033217v1.full.pdf
>
> Martin
>
>
> On Mon, Mar 16, 2020 at 5:44 AM Dave Johnston <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I'm with Andreas on this, there may be asymptomatic carriers out there
>> (including unit staff), we just don't know, so have to assume the potential
>> that any part of the scopes that get any human contact could be
>> contaminated, and we consider the scopes and most other ancillary
>> instruments to effectively be impossible to decontaminate without risk of
>> damage, hence fresh gloves on entering the unit, that can be sprayed with
>> 70% ethanol on regular basis, prevents you contaminating or being
>> contaminated. We have confocals, TEMs, SEMs, slide scanners and analysis
>> PCs to name a few and are based in a large teaching hospital site, so are
>> also thinking about trying to encourage remote access to analysis PCs to
>> minimise footfall and movement around the unit and wider site. That being
>> said, have yet to convince the boss to adopt those policies.
>>
>> On the plus side, given reported viability on surfaces, a first thing in
>> the morning clean and last thing at night clean may be unnecessary as if
>> any virus at end of day lock up, will be inactive by morning? Between user
>> cleaning may be best but we can have several dozen users in at any one time
>> moving between instruments and don't want to give them access to spray
>> bottles!!!!!
>>
>> Dave Johnston, Biomedical Imaging, Southampton.
>>

... [show rest of quote]

Angela Kurz-2

Mar 18, 2020; 12:14am

Re: COVID19: Instrument cleaning protocols: Recommendations by German BioImaging

In reply to this post by Nadine Utz

Nadine Utz

Mar 18, 2020; 3:22pm

Re: COVID19: Instrument cleaning protocols: Recommendations by German BioImaging

In reply to this post by Nadine Utz

*****
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Dear all,

since last Friday, when the Recommendations by German BioImaging were
discussed within workshop participants, the situation has changed, at
least in Germany. Schools and kindergardens are closed, and also many
universities and research institutes.

A discussion within the German BioImaging community took place since the
publication of the recommendations yesterday, and resulted in revised
recommendations and the following preamble to the document:

"Core facilities are shared scientific infrastructures. Users come from
different departments or institutions to operate the same equipment and
take advantage of scientific services provided by a limited number of
facility staff. In this setting, transmission of the SARS-CoV- 2 virus
between individuals or from contaminated surfaces is facilitated.
Therefore, core facilities deserve special attention from the research
institutions ́ higher management. As a group of experienced core
facility leaders, we strongly recommend ceasing all operations in core
facilities, with highest priority to those requiring personal
interaction, and urge our colleagues to convince their institutions to
follow this advice. Closure of the facility is the only measure that
ensures users and staff safety. Even in regions with a low number of
known infections, the time to act is now.

The guidelines described below should help imaging core managers to
protect themselves and their users a) if running the facility is made
mandatory despite the current emergency, and b) for resuming operations
in a post peak situation as safely as possible."

Please find the revised document here:

https://www.gerbi-gmb.de/Corona

Best wishes

Nadine

Am 17.03.20 um 17:08 schrieb Nadine Utz:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Core Facility Managers, who attended the "Trends in Microscopy - German
> BioImaging Spring School" last week, held last Friday a spontaneous
> workshop to discuss precautions of imaging core facilities to prevent
> COVID19 infection of staff and users. The outcome of this workshop has
> been summarized in recommendations by German BioImaging. I could not
> include an attachment to this email and therefor uploaded the document
> to this web site:
>
> https://www.gerbi-gmb.de/Corona
>
> I would like to express my sincere thanks to the participants of the
> workshop:
>
> Steffen Dietzel, Ludwig Maximilian University of Munich
> Hans Fried, German Center for Neurodegenerative Diseases (DZNE) in Bonn
> Angela Naumann, University of Freiburg
> Roland Nitschke, University of Freiburg
> Pawel Pasierbeck, IMBA Institute of Molecular Biotechnology in Vienna
> Britta Schroth-Diez, MPI of Molecular Cell Biology and Genetics
> Martin Stoeckl, University of Konstanz
> Stefan Terjung, European Molecular Biology Laboratory in Heidelberg
> Roland Thuenauer, Centre for Structural Systems Biology in Hamburg
> Silke Tulok, Technical University of Dresden
>
> Workshop discussions were mainly based on:
> - this confocal mailing list discussion
> - recommendations by the World Health Organization
> - recommendations by the Robert Koch Institute (German federal
> government agency and research institute responsible for disease control
> and prevention)
>
> Best wishes
> Nadine
>

... [show rest of quote]

--
Dr. Nadine Utz
Managing Director

German BioImaging - Gesellschaft für Mikroskopie und Bildanalyse e.V.
German BioImaging - Society for Microscopy and Image Analysis c/o University of Konstanz
Box 604
78457 Konstanz, Germany
Phone +49 7531 885337
[hidden email]
http://www.gerbi-gmb.de

F Javier Diez Guerra

Mar 18, 2020; 3:55pm

Re: COVID19: Instrument cleaning protocols

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,

During the last days, we have listened to the many and useful advice
from confocalists all over the world. We are delighted and very proud to
belong to this confocal microscopy community.

Starting last Monday our core facility at the CBMSO in Madrid is closed.
No technical personnel and no granted access to the microscopes. Here,
we are convinced that only adopting such radical measures, we'll be able
to slow down the spread of covid-19.

In the meantime, as a core facility team, we are now trying to analyze
and learn as much as possible from this experience to be better prepared
for the future.

Hope you all are well and continue to be so during this uncertain time.

I really needed to spell out my most heartfelt thanks to all the
contributors.

Best wishes

Javier

--
Fco. Javier Diez-Guerra, PhD

Servicio de Microscopía Confocal
Centro de Biologia Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Campus de Cantoblanco
28049 Madrid
SPAIN

Tel +34 91 196 4612
e-mail: [hidden email]

El 18/03/2020 a las 16:22, Nadine Utz escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> since last Friday, when the Recommendations by German BioImaging were
> discussed within workshop participants, the situation has changed, at
> least in Germany. Schools and kindergardens are closed, and also many
> universities and research institutes.
>
> A discussion within the German BioImaging community took place since the
> publication of the recommendations yesterday, and resulted in revised
> recommendations and the following preamble to the document:
>
> "Core facilities are shared scientific infrastructures. Users come from
> different departments or institutions to operate the same equipment and
> take advantage of scientific services provided by a limited number of
> facility staff. In this setting, transmission of the SARS-CoV- 2 virus
> between individuals or from contaminated surfaces is facilitated.
> Therefore, core facilities deserve special attention from the research
> institutions ́ higher management. As a group of experienced core
> facility leaders, we strongly recommend ceasing all operations in core
> facilities, with highest priority to those requiring personal
> interaction, and urge our colleagues to convince their institutions to
> follow this advice. Closure of the facility is the only measure that
> ensures users and staff safety. Even in regions with a low number of
> known infections, the time to act is now.
>
> The guidelines described below should help imaging core managers to
> protect themselves and their users a) if running the facility is made
> mandatory despite the current emergency, and b) for resuming operations
> in a post peak situation as safely as possible."
>
> Please find the revised document here:
>
> https://www.gerbi-gmb.de/Corona
>
> Best wishes
>
> Nadine
>
>
>

... [show rest of quote]

Chloe van Oostende

Mar 22, 2020; 8:53pm

Re: COVID19: Instrument cleaning protocols

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi,
For Keyboards, mouse and focus knob, around the oculars, I use Decon™
Conflikt™ Detergent Disinfectant
It doesn't damaged plastic.
For oculars themselves, I use 70% ethanol.
Best
Chloe
*Chloë van Oostende, PhD*

Facility manager- Senior Microscopy specialist
*Cell Biology and Image Acquisition Core Facility*
Faculty of Medicine
University of Ottawa
RGN 3171
451 Smyth Road
Ottawa, ON K1H 8M5
Office: 613-562-5800 ext: 8376

http://www.chloevanoostende.net
CBIA core facility
<http://www.med.uottawa.ca/research/corelabs/CoreLabs_CBIA/eng/>

Le mer. 18 mars 2020 à 11:56, F Javier Diez Guerra <[hidden email]> a
écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> During the last days, we have listened to the many and useful advice
> from confocalists all over the world. We are delighted and very proud to
> belong to this confocal microscopy community.
>
> Starting last Monday our core facility at the CBMSO in Madrid is closed.
> No technical personnel and no granted access to the microscopes. Here,
> we are convinced that only adopting such radical measures, we'll be able
> to slow down the spread of covid-19.
>
> In the meantime, as a core facility team, we are now trying to analyze
> and learn as much as possible from this experience to be better prepared
> for the future.
>
> Hope you all are well and continue to be so during this uncertain time.
>
> I really needed to spell out my most heartfelt thanks to all the
> contributors.
>
> Best wishes
>
> Javier
>
> --
> Fco. Javier Diez-Guerra, PhD
>
> Servicio de Microscopía Confocal
> Centro de Biologia Molecular Severo Ochoa
> C/ Nicolás Cabrera, 1
> Campus de Cantoblanco
> 28049 Madrid
> SPAIN
>
> Tel +34 91 196 4612
> e-mail: [hidden email]
>
>
>
> El 18/03/2020 a las 16:22, Nadine Utz escribió:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear all,
> >
> > since last Friday, when the Recommendations by German BioImaging were
> > discussed within workshop participants, the situation has changed, at
> > least in Germany. Schools and kindergardens are closed, and also many
> > universities and research institutes.
> >
> > A discussion within the German BioImaging community took place since the
> > publication of the recommendations yesterday, and resulted in revised
> > recommendations and the following preamble to the document:
> >
> > "Core facilities are shared scientific infrastructures. Users come from
> > different departments or institutions to operate the same equipment and
> > take advantage of scientific services provided by a limited number of
> > facility staff. In this setting, transmission of the SARS-CoV- 2 virus
> > between individuals or from contaminated surfaces is facilitated.
> > Therefore, core facilities deserve special attention from the research
> > institutions ́ higher management. As a group of experienced core
> > facility leaders, we strongly recommend ceasing all operations in core
> > facilities, with highest priority to those requiring personal
> > interaction, and urge our colleagues to convince their institutions to
> > follow this advice. Closure of the facility is the only measure that
> > ensures users and staff safety. Even in regions with a low number of
> > known infections, the time to act is now.
> >
> > The guidelines described below should help imaging core managers to
> > protect themselves and their users a) if running the facility is made
> > mandatory despite the current emergency, and b) for resuming operations
> > in a post peak situation as safely as possible."
> >
> > Please find the revised document here:
> >
> > https://www.gerbi-gmb.de/Corona
> >
> > Best wishes
> >
> > Nadine
> >
> >
> >
>

... [show rest of quote]