Carolina Workshop on Biosensors and Force Measurements in Living Cells -Auguest 9-12

[Tim O'Brien Sr.]

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Hi-


I wanted to let everyone know that we will be presenting our 8th workshop on the application of, and assessment of  responses to, forces on living cells this August 9-12th.  This year is the first that our course will incorporate the use of FRET-based biosensors and light modulated signaling molecules from the laboratory of Klaus Hahn, and the application of our vertical light sheet and pathway rotated imaging for sideways microscopy (PRISM) techniques.  These are in combination with AFM and magnetic tweezers.  There will be morning lectures and afternoon, hands-on labs.  A highlight this year will be guest speaker Teng-Leong Chew from HHMI Janelia Research Center, who will present two morning sessions on image analysis using the ImageJ/FIJI toolbox.  The blurb below describes the course in more detail, and how to apply?.


Please tell students and colleagues that might be interested!


Thanks,


Tim O'Brien

UNC CISMM

Chapel Hill, NC

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Carolina Workshop on Biosensors and Force Measurements in Living Cells

The eighth annual Carolina Workshop on Biosensors and Force Measurements in Living Cells is scheduled for August 9th - 12th, 2016.

Cells respond to external biochemical and mechanical cues to generate myriad responses including force generation, motility, gene expression and differentiation. The connection between external cue and internal response are the biochemical pathways that control the cell. Understanding these responses is critical to gaining insight into diverse biomedical phenomena such as cancer, immunity and tissue differentiation and morphogenesis. Our workshop will offer, through morning lectures and afternoon laboratory sessions, ways to assess the biochemistry and mechanobiology of cells, and the theoretical basis for interpreting physical properties and force sensing and generation.  We will cover the use of FRET-based biosensors related to cell mechanics and responses, such as RhoA and Rac1, plus light-modulated signaling molecules. We will present the optics and plans for assembling your own versatile light control microscopy systems that includes vertical light sheet microscopy, spinning total internal reflection fluorescence (TIRF) and photoactivation capability.  New to this year's workshop will be two morning sessions on image analysis using the FIJI toolbox, taught by guest speaker Teng-Leong Chew from HHMI Janelia Research Center. Experimental stations will include atomic force microscopy combined with light sheet microscopy, 3D force microscopy (magnetic tweezers) with TIRF, the use of a high throughput, 12 channel microscope for cell rheology and FRET microscopy using fluorescent biosensors.  This year we have reduced our registration fee to $100, and this includes all supplies, light breakfasts, and a conference dinner/poster session.  The workshop is hosted by CISMM, our NIH/NIBIB resource (Center for Computer Integrated Systems for Microscopy and Manipulation).   To Register and for more information: http://cismm.web.unc.edu/resources/events//<http://cismm.web.unc.edu/resources/events/>


Application Deadline is August 1, 2016  ??