Cell tracer with far red emission

[Louis Villeneuve]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi everyone,

We would need a live cell tracer reagent  (tissue will be fixed after) that would be excited either with a 543 nm or a 633nm laser line.  Emission of the dye should be over 640 nm in order to seperate it from my rhodamine counterstain.  I am using an "old" LSM 510.  

The tracer would have minimal effect on cell migration, adhesion, and proliferation.

Any suggestions?

Louis

Louis Villeneuve
Research Associate- Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

[hidden email]

[Dries Vercauteren]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Louis,

do you think 10kDa dextrane, conjugated with AlexaFluor647 could do the trick? This compound will be aspecificlly taken up via endocytosis and will therefore eventually label all your cells without affecting them... Moreover, it's fixable with paraformaldehyde..
Though, it won't give you a homogenous labeling of your cell..

You can buy this at Mol Probes, No commercial interest!

Good luck,

Dries

On 26/03/2008, Louis Villeneuve <[hidden email]> wrote:

Search the CONFOCAL archive at <a href="http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal" target="_blank" onclick="return top.js.OpenExtLink(window,event,this)">http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi everyone,

We would need a live cell tracer reagent  (tissue will be fixed after) that would be excited either with a 543 nm or a 633nm laser line.  Emission of the dye should be over 640 nm in order to seperate it from my rhodamine counterstain.  I am using an "old" LSM 510.  

The tracer would have minimal effect on cell migration, adhesion, and proliferation.

Any suggestions?

Louis

Louis Villeneuve
Research Associate- Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

[hidden email]

--
Dries Vercauteren, PhD student
Master of Bioscience Engineering: Cell and Gene Biotechnology

Ghent Research Group on Nanomedicines
www.ugent.be/fw/en/research/biofys
Faculty of pharmaceutical sciences, Ghent University
Harelbekestraat 72, 9000 Ghent
Belgium

Phone:  [phone]
Mobile: [phone]
E-mail: [hidden email]
           [hidden email]

[Ignatius, Mike]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Louis,

 

CellTrace Far Red DDAO-SE (cat #C34553) excites at ~633 and emits > 650.  It is designed for live cell work.  The SE attaches it to free amines in the cell, thus facilitating fixation as well.

 

To minimize effects on cellular function, as with any dye, less is best - in terms of dye and light used to image. 

 

Mike Ignatius

 

Molecular Probes/Invitrogen

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Louis Villeneuve
Sent: Wednesday, March 26, 2008 7:11 AM
To: [hidden email]
Subject: Cell tracer with far red emission

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi everyone,

We would need a live cell tracer reagent  (tissue will be fixed after) that would be excited either with a 543 nm or a 633nm laser line.  Emission of the dye should be over 640 nm in order to seperate it from my rhodamine counterstain.  I am using an "old" LSM 510.  

The tracer would have minimal effect on cell migration, adhesion, and proliferation.

Any suggestions?

Louis

Louis Villeneuve
Research Associate- Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

[hidden email]