Cholesterol dyes

[Sarah Rolling]

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Hello all!!

I have a user who is wanting to stain his cholesterol in some muscle tissue for
a fixed slide.  He already has filipin which works alright in a fluorescence
microscope, but the problem with our imaging is that we are not equipped with
the correct laser for excitation.

So basically I was wondering if anyone knows of a green, red, or far red dye
that can be used to stain cholesterol in fixed muscle tissue.

Sarah Rolling

[Nuno Moreno]

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To my knowledge there are 3 things that you can use

Laurdan

di 4 ANNEPSHQ for membrane fluidity - less fluidity more sterols -
ratiometric

cholera toxin CT-B - several Alexas

I must point out that filipin III is actually the worst since is
extremely toxic and and bleaches very fast.

Regards,
NM

PS: I have no commercial interest but they can be purchased from
Molecular Probes/invitrogen


Sarah Rolling wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello all!!
>
> I have a user who is wanting to stain his cholesterol in some muscle tissue for
> a fixed slide.  He already has filipin which works alright in a fluorescence
> microscope, but the problem with our imaging is that we are not equipped with
> the correct laser for excitation.
>
> So basically I was wondering if anyone knows of a green, red, or far red dye
> that can be used to stain cholesterol in fixed muscle tissue.
>
> Sarah Rolling
>

--
Nuno Moreno
Cell Imaging Unit
Instituto Gulbenkian de Ciência
http://uic.igc.gulbekian.pt
http://www.igc.gulbekian.pt
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