Chromosome painting

[Camilla Margaroli]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello everyone,


I was wondering if any of you has ever done chromosome painting to see the chromosome location in the nucleus. If yes, could you let me know which probes did you use and if you did it yourself or if you sent it elsewhere to do it?

It will be my first time doing this staining.

Thanks for your help!

Best,

Camilla

[Cesare -ALEMBIC-]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear List,
we are currently perfoming FRAP experiments using a Leica SP8 with LAS X version software.
In our experment, we need to follow the fluorescence recovery for several tens of minutes in a very specific focal plane (proteins related to focal adhesions).
We are using the FRAP app wizard and we would like to use the AFC (Adaptative Focus Control) to keep the focal adhesions in focus but FRAP wizard doesn't provide to use it and it's not available as option. We tried to set AFC directly on the microscope but when we start the run, it's immediatly swtiched off.
Does anybody have the same problem and known how to use AFC in FRAP experiment?
Thanks in advance!

Have a nice day,

Cesare


Facility Manager
ALEMBIC
Advanced Light and Electron Microscopy BioImaging Center
San Raffaele Scientific Institute
DIBIT 1  S2B4a
via Olgettina 58, 20132 - Milano - Italy
Tel [phone](others -4640, -4641)
Fax [phone]
e-mail: [hidden email]<mailto:[hidden email]>
ALEMBIC webpage: www.hsr.it/research/alembic<http://www.hsr.it/research/alembic>

--
<http://www.hsr.it/research/alembic>


Rispetta l’ambiente: non stampare questa mail se non è necessario.
Respect the environment: if it's not necessary, don't print this mail.

IL TUO 5XMILLE AL SAN RAFFAELE DI MILANO

Devolvi il tuo 5 per mille all’Ospedale San Raffaele: perché al centro della Ricerca ci sei TU. CODICE FISCALE: 07636600962, nel riquadro RICERCA SANITARIA. Non c’è cura, senza ricerca. Non c’è ricerca, senza il tuo 5xmille. Scopri come su http://www.5xmille.org

[http://www.5xmille.org/img/731x129.jpg]

[Steffen Dietzel]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Camilla,

there is a rather large body of literature on FISH in interphase nuclei.
If you search Pubmed for contributions by the groups of Wendy Bickmore,
Thomas Cremer, or Tom Misteli, this would be a good starting point.
There are also several papers or protocols on 'how-to' or 'how not to', eg.

https://www.ncbi.nlm.nih.gov/pubmed/18951171

https://www.ncbi.nlm.nih.gov/pubmed/16954680

and maybe one of my own:

https://www.ncbi.nlm.nih.gov/pubmed/17119992


'Back in the days', I always used non-commercial, nick-translation
labeled probes. I would assume this may have changed in the mean time, I
am currently not working in this field. Whether you are using a BAC
clone or a chromosome paint as probe does not make a principal
difference. you just need more DNA for the latter.

Steffen



Am 16.06.2016 um 15:02 schrieb Camilla Margaroli:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
>
> I was wondering if any of you has ever done chromosome painting to see the chromosome location in the nucleus. If yes, could you let me know which probes did you use and if you did it yourself or if you sent it elsewhere to do it?
>
> It will be my first time doing this staining.
>
> Thanks for your help!
>
> Best,
>
> Camilla
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de