Claire's new Mercury Free Microscopy awareness campaign article online

[George McNamara]

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Dear listserv,

Check out the attached Mercury Free Microscopy awareness campaign article.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970759/


JBt is the house journal of ABRF (www.abrf.org). Has three publication
advantages:
1. hosted by PubMed - so articles show up in PubMed (It is peer reviewed).
2. Gold open access (free for everyone to read).
3. No publication charges.

Enjoy,

George


--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42

[Vitaly Boyko]

Dear All,

Could you, please comment on your experience with diff
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Dear All,

Could you, please comment on your experience with different LED illuminators.
Is there one that covers both UV (340 nm LED for Fura-2 Ca imaging), and near-IR?
I know that Q-photonics has one at 330 nm (15 mW and 50 mW; please find the link below). Does anyone have any experience incorporating them into any LED based illumination device?

UVCLEAN: deep UV LED at 330nm

 
            
UVCLEAN: deep UV LED at 330nm
deep UV LED, deep UV sources, SETi distributor, UV light emitting diodes, UVCLEAN, LED 255nm, LED 265nm, LED 275nm, LED 280nm, LED 290nm, LE...  
View on www.qphotonics.com Preview by Yahoo  
 
 
Thank you,

Vitaly

[James Kerin]

Dear Vitaly, We've not used them ourselves, but the 330nm LEDs from Q-Photonics seem (from the data sheet) to be constructed from an array of small LEDs to give a relatively large emitting area.  This is OK for illuminating large samples using a condenser lens (we are aware of groups using similar devices for macro imaging), but is not ideal for episcopic illumination through the microscope objective where point intensity is more important than overall photon flux. Unfortunately we are not aware of anyone producing an indvidual high power 340nm LED with an emitting area of less than 3 mm. We provide Fura 2 LED-based illuminators by spectrally selecting the lower bandpass section of a high-power 365nm LED.  This requires precise regulation of the filtered output signal, but our control electronics include this photodiode feedback and optical monitoring functionality as standard in any case.  The resultant bandpass is centred at slightly longer than
340nm, but is sufficiently below the isosbestic point to give a high calcium transient and allow effective ratio calcium imaging.  


http://www.cairn-research.co.uk/catalogue/illumination/led-light-sources/product/optoled-light-source-0


Please let us know if you have further questions. 





 
 
 




J. P.  Kerin
Marketing Director
Cairn Research  Ltd
Graveney Road
Faversham
Kent, ME13 8UP
UK


www.cairn-research.co.uk
[hidden email]

Tel: + 44 (0)1795  594507
Fax: + 44 (0)1795 594510

 

 

 




On  07/08/2014, 07:52pm, Vitaly Boyko ([hidden email]) wrote:
Dear All,
 

 
Could you, please comment on your experience with diff
 
*****
 
To join, leave or search the confocal microscopy listserv, go to:
 
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
 
Post images on http://www.imgur.com and include the link in your posting.
 
*****
 

 

 

 
Dear All,
 

 
Could you, please comment on your experience with different LED illuminators.
 
Is there one that covers both UV (340 nm LED for Fura-2 Ca imaging), and near-IR?
 
I know that Q-photonics has one at 330 nm (15 mW and 50 mW; please find the link below). Does anyone have any experience incorporating them into any LED based illumination device?
 

 
UVCLEAN: deep UV LED at 330nm
 

 
 
 
            
 
UVCLEAN: deep UV LED at 330nm
 
deep UV LED, deep UV sources, SETi distributor, UV light emitting diodes, UVCLEAN, LED 255nm, LED 265nm, LED 275nm, LED 280nm, LED 290nm, LE...  
 
View on www.qphotonics.com Preview by Yahoo  
 
 
 
 
 
Thank you,
 

 
Vitaly

[Craig Brideau]

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*****

If you want to use fiber optic based deliver, also keep in mind that many
glasses used in fiber optics do not transmit wavelengths shorter than
~400nm very well.

Craig


On Fri, Jul 11, 2014 at 12:44 PM, James Kerin <[hidden email]>
wrote:

> Dear Vitaly,&nbsp;We've not used them ourselves, but the 330nm LEDs from
> Q-Photonics seem (from the data sheet) to be constructed from an array of
> small LEDs to give a relatively large emitting area. &nbsp;This is OK for
> illuminating large samples using a condenser lens (we are aware of groups
> using similar devices for macro imaging), but is not ideal for episcopic
> illumination through the microscope objective where point intensity is more
> important than overall photon flux. Unfortunately we are not aware of
> anyone producing an indvidual high power 340nm LED with an emitting area of
> less than 3 mm.&nbsp;We provide Fura 2 LED-based illuminators by spectrally
> selecting the lower bandpass section of a high-power&nbsp;365nm&nbsp;LED.
> &nbsp;This requires precise regulation of the filtered output signal, but
> our control electronics include this photodiode feedback and optical
> monitoring functionality as standard in any case. &nbsp;The resultant
> bandpass is centred at slightly longer than
> 340nm, but is sufficiently below the isosbestic point to give a high
> calcium transient and allow effective ratio calcium imaging. &nbsp;
>
>
>
> http://www.cairn-research.co.uk/catalogue/illumination/led-light-sources/product/optoled-light-source-0
>
>
> Please let us know if you have further questions.&nbsp;
>
>
>
>
>
>
>
>
>
>
>
>
> J. P.  Kerin
> Marketing Director
> Cairn Research  Ltd
> Graveney Road
> Faversham
> Kent, ME13 8UP
> UK
>
>
> www.cairn-research.co.uk
> [hidden email]
>
> Tel: + 44 (0)1795  594507
> Fax: + 44 (0)1795 594510
>
>
>
> &nbsp;
>
> &nbsp;
>
>
>
>
> On  07/08/2014, 07:52pm, Vitaly Boyko ([hidden email]) wrote:
> Dear All,
>
>
>
> Could you, please comment on your experience with diff
>
> *****
>
> To join, leave or search the confocal microscopy listserv, go to:
>
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
> Post images on http://www.imgur.com and include the link in your posting.
>
> *****
>
>
>
>
>
>
>
> Dear All,
>
>
>
> Could you, please comment on your experience with different LED
> illuminators.
>
> Is there one that covers both UV (340 nm LED for Fura-2 Ca imaging), and
> near-IR?
>
> I know that Q-photonics has one at 330 nm (15 mW and 50 mW; please find
> the link below). Does anyone have any experience incorporating them into
> any LED based illumination device?
>
>
>
> UVCLEAN: deep UV LED at 330nm
>
>
>
> &nbsp;
>
> &nbsp;  &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
>
> UVCLEAN: deep UV LED at 330nm
>
> deep UV LED, deep UV sources, SETi distributor, UV light emitting diodes,
> UVCLEAN, LED 255nm, LED 265nm, LED 275nm, LED 280nm, LED 290nm, LE...
>
> View on www.qphotonics.com Preview by Yahoo
>
> &nbsp;
>
> &nbsp;
>
> Thank you,
>
>
>
> Vitaly
>