Clearing a sea slug (Elysia rufescens)

[Gary Laevsky]

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Hi All,

We been working on this for  a few months, and not getting what we need.

The goal is to image the whole critter on one of our light-sheets.

We've tried CUBIC, and iDisco (using DCM and Ethyl Cinnamate).

Any ideas?

Thanks so much.

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.

[Arvydas Matiukas]

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Hi Gary,

How big is your critter (sorry just noticed the name in the subject but no dimensions provided)and what type of tissues/cells does it contain? I was able to clear heart=muscle tissue up to 10mm thick using the old good  BABB (though not compatible with many dyes and FP). The modern clearing methods are good for brain but not working on the heart because of significantly higher refractive index.

Best,
Arvydas





+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Research Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University


>>> Gary Laevsky <[hidden email]> 02/10/20 8:10 AM >>>
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Hi All,

We been working on this for  a few months, and not getting what we need.

The goal is to image the whole critter on one of our light-sheets.

We've tried CUBIC, and iDisco (using DCM and Ethyl Cinnamate).

Any ideas?

Thanks so much.

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://urldefense.proofpoint.com/v2/url?u=https-3A__namsmicroscopy.com_&d=DwIBaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=dYD-JqP3bCJyPD3yje4UrA1paF0oSWgb9nsZ5N4MKWA&s=KtC4lMFaev7ApILM9jF34BqK3BiwijmfniRe3ZXMEKA&e= 
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.