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Dear All,
I am considering use of Clover and mRuby2 (Lam et al., Nature Methods
9, 1005–1012(2012 doi:10.1038/nmeth.2171) as fluorescent proteins pair
in FLIM-FRET experiments. The problem is that in my FLIM setup I have
470 nm pulsed laser line whereas Clover has 505 nm ex. max. Do you have
any experience with Clover ex. at 470nm and/or Clover in FLIM applications?
Best,
Michał
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