Coherent OPSL 514nm solid state laser RE: FRET in the time of DPSS

[Jennifer Clarke]

Dear list

I have just obtained a quote from Coherent for an Optically Pumped Semiconductor Laser (OPSL) Sapphire Laser at 514nm for exactly this purpose of CFP/YFP FRET.
I am hoping (but yet to find out) that it will be compatible to install on an Olympus FV1000

I think its a new product and may not yet be on their website.

I would be very interested to hear if anyone on the list has already purchased one and can provide any comment?
Or know of any other 514nm solid state lasers, in case this one turns out not to be compatible with installation on our FV1000

Coherent also now do a 488nm Sappire Laser

(No commercial interest)
Jen
--
Jennifer Clarke BSc (Hons) PhD
Research Associate, Anatomy and Histology
Centre for Neuroscience, School of Medicine
&
Facility Manager, Optical Microscopy Suite, Flinders Microscopy

Flinders University
GPO Box 2100, Adelaide 5001
Phone: [phone] 8204 6637
Email: [hidden email]
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Damir Sudar [[hidden email]]
Sent: Thursday, 10 November 2011 10:51 AM
To: [hidden email]
Subject: Re: FRET in the time of DPSS

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See:
http://www.cobolt.se/coboltfandango515nm.html?gclid=CPCOueXlqqwCFQyEhwodQn-aAw

No connection, just considering about switching away from our old Ar Ion
as well.

- Damir

On 11/9/2011 3:36 PM, Guy Cox wrote:

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>
> Are you sure there is a 514nm DPSS?  I think that wavelength would have
> to be an OPS and I don't know anyone who makes one.  Coherent do make a
> 505nm OPS which is intended to stand in for both the 488&  514 lines of
> an Argon laser, and I rather suspect that this could be exactly what you
> need.  But I have no experience of it.  (I do have a Coherent 488nm
> OPS).
>
>                                         Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor&  Francis
>       http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Australian Centre for Microscopy&  Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
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>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Tim Feinstein
> Sent: Thursday, 10 November 2011 7:34 AM
> To: [hidden email]
> Subject: FRET in the time of DPSS
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello all,
>
> We want to spec a four-laser launch for a new live cell system that will
> handle both CFP/YFP FRET and red/green imaging.   However, I am sad to
> see that gas lasers are no longer speccable and so the freebie 514 laser
> line is gone.  We would therefore have to spec a 514 DPSS and forego the
> far-red line.
>
> I was wondering whether there is a way to do more (or at least the same)
> with less.  488 nm excites YFP well enough, so in theory I could image
> CFP/YFP using a scan head dichroic with cutouts for 442 and 488 nm laser
> lines.  In my experience 442 nm laser excitation (via TIRF) causes
> negligible YFP excitation and 488 nm does not excite CFP, so it is
> possible that I could gain speed by passing everything through a single
> broad bandpass filter (e.g., 455-550 nm) and alternate excitations.
> Assuming that cross-talk is not a problem, the most significant cost
> would be that I lose a decent chunk of CFP emission to the scan head
> dichroic, but in return I gain a 641 nm laser.
>
> Has anyone tried this?  Any feedback on or off-list would be much
> appreciated.
>
> Thanks and all the best,
>
>
> TF
>
> Timothy Feinstein, PhD
> Postdoctoral Fellow
> Laboratory for GPCR Biology
> Dept. of Pharmacology&  Chemical Biology
> University of Pittsburgh, School of Medicine
> BST W1301, 200 Lothrop St.
> Pittsburgh, PA  15261
>
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--
Damir Sudar - Staff Scientist and Deputy for Technology
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
WWW: http://www.lbl.gov/lsd/People_&_Organization/Scientific_Staff_Directory/Sudar_Lab.html