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QUANTITATIVE IMAGING:FROM CELLS TO MOLECULES
Cold Spring Harbor Laboratory
April 8 - 21, 2015
Application Deadline: January 31, 2015
Instructors:
Jennifer Waters, Harvard Medical School
Torsten Wittmann, University of California San Francisco
Hunter Elliott, Harvard Medical School
This course will focus on advanced quantitative fluorescence microscopy
techniques used for imaging a range of biological specimens, from cells to single
molecules. The course is designed for cell and molecular biologists with little or no
microscopy experience who wish to begin utilizing microscopy in their own
research. Students will gain a theoretical understanding of, and hands-on
experience with, state-of-the-art equipment used in quantitative fluorescence
microscopy, including: wide-field fluorescence microscopy, laser scanning and
spinning disk confocal microscopy, total internal reflection fluorescence microscopy
(TIRF), super-resolution methods (structured illumination, STED, STORM and
PALM) and digital image processing and analysis. Students will learn how to design
and implement a wide range of imaging experiments using these techniques,
including live specimens and optically activatable probes. Students will use the
techniques to address specific quantitative questions and then discuss the results
as a group, learning to troubleshoot the common problems that occur in the course
of a quantitative imaging experiment. Among the topics presented are:
quantitative microscopy basics, transmitted light microscopy, basic image
processing and analysis, CCD & sCMOS cameras, confocal microscopy, multi-
photon microscopy, deconvolution, TIRF, imaging ratio-metric "biosensors"
(including FRET), light sheet microscopy and super-resolution techniques. Students
will also learn guidelines for choosing fluorescent proteins, and work with live
samples requiring environmental control. In addition to the course instructors
listed above, lecturers for 2015 include Nathan Shaner (The Scintillon Institute),
Talley Lambert (Harvard Medical School), Dylan Burnette (Vanderbilt), and Pavel
Tomancak (Max Planck Institute of Molecular Cell Biology & Genetics).
This course is supported with funds provided by the National Cancer Institute.
Many students receive financial support from CSHL to cover course tuition.
For more information and to apply, visit:
http://meetings.cshl.edu/courses/2015/c-qicm15.shtml
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