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Dear All,
This upcoming webinar will be educational with very little commercial
content (but will feature Zeiss LSM510 confocal system and Visiopharm's
newCAST stereology software to illustrate the scientific principles of
confocal stereology).
Thank you.
Won Yung Choi, Ph.D.
Senior Product Scientist
Visiopharm USA (New York City Office)
917-464-5633
Confocal Stereology: The Quantitative Approach to Immunofluorescence
Please join us for a Webinar on February 22
Space is limited.
Reserve your Webinar seat now at:
https://www1.gotomeeting.com/register/365851585By Dr. Won Yung Choi, Senior Products Scientist, Visiopharm
Confocal laser scanning microscopy is widely used in biological sciences for
improved optical resolution and contrast. This is obtained by illuminating
the specimen with a focused scanning laser beam and by placing a pinhole
aperture in the image plane in front of the electronic photon detector.
Confocal images can also be produced by the use of spinning disks that
perform tandem scans with multiple scanning beams. Both types of techniques
result in rejection of signals from above and below the focal plane,
producing clear, high-contrast images that are used for qualitative analysis
of the specimen.
Unfortunately, many researchers do not realize that it is possible - and
desirable - to obtain an accurate, unbiased quantitative estimation on a
confocal microscope setup. As a result, most scientists resort to either
(1) a biased estimation by subjectively selecting fields of view from which
to quantify (and spending too much time performing inaccurate
quantification), or (2) producing visually pleasing images (which only
demonstrate the qualitative aspects of their research questions).
In this scientific presentation, a typical workflow using newCAST stereology
software on a Zeiss LSM 510 confocal laser scanning microscope will be
demonstrated for unbiased quantitative analysis on sampling fields that are
correctly obtained using the principles of systematic uniform random
sampling. Examples will include stereological estimation of (i) number of
cells, (ii) length density of fibers, and (iii) volume of tissue.
Title: Confocal Stereology: The Quantitative Approach to Immunofluorescence
Date: Tuesday, February 22, 2011
Time: 9:00-10:00 AM Eastern Standard Time / 3:00 4:00 Central European Time
After registering you will receive a confirmation email containing
information about joining the Webinar.
System Requirements
PC-based attendees
Required: Windows® 7, Vista, XP or 2003 Server
Macintosh®-based attendees
Required: Mac OS® X 10.4.11 (Tiger®) or newer
Please let us know if you wish to unsubscribe to our webinar invitations.
With kind regards,
Lene Gerlach
VP Business Development
Visiopharm
[hidden email]
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